Oriented Immobilization of [NiFeSe] Hydrogenases on Covalentlyand Noncovalently Functionalized Carbon Nanotubes for H2/AirEnzymatic Fuel Cells

International audienceWe report the oriented immobilization of [NiFeSe] hydrogenases on both covalently and noncovalently modified carbon nanotubes (CNTs) electrodes. A specific interaction of the [NiFeSe] hydrogenase from Desulfomicrobium baculatum with hydrophobic organic molecules was probed by electrochemistry, quartz crystal microbalance with dissipation monitoring (QCM-D), and theoretical calculations. Taking advantage of these hydrophobic interactions, the enzyme was efficiently wired on anthraquinone and adamantane-modified CNTs. Because of rational immobilization onto functionalized CNTs, the O-2-tolerant [NiFeSe]-hydrogenase is able to efficiently operate in a H-2/air gas-diffusion enzymatic fuel cell

Gold–Carbon Nanocomposites for Environmental Contaminant Sensing

The environmental crisis, due to the rapid growth of the world population and globalisation, is a serious concern of this century. Nanoscience and nanotechnology play an important role in addressing a wide range of environmental issues with innovative and successful solutions. Identification and control of emerging chemical contaminants have received substantial interest in recent years. As a result, there is a need for reliable and rapid analytical tools capable of performing sample analysis with high sensitivity, broad selectivity, desired stability, and minimal sample handling for the detection, degradation, and removal of hazardous contaminants. In this review, various gold–carbon nanocomposites-based sensors/biosensors that have been developed thus far are explored. The electrochemical platforms, synthesis, diverse applications, and effective monitoring of environmental pollutants are investigated comparatively

Oriented Immobilization of [NiFeSe] Hydrogenases on Covalently and Noncovalently Functionalized Carbon Nanotubes for H₂/Air Enzymatic Fuel Cells

We report the oriented immobilization of [NiFeSe] hydrogenases on both covalently and noncovalently modified carbon nanotubes (CNTs) electrodes. A specific interaction of the [NiFeSe] hydrogenase from <i>Desulfomicrobium baculatum</i> with hydrophobic organic molecules was probed by electrochemistry, quartz crystal microbalance with dissipation monitoring (QCM-D), and theoretical calculations. Taking advantage of these hydrophobic interactions, the enzyme was efficiently wired on anthraquinone and adamantane-modified CNTs. Because of rational immobilization onto functionalized CNTs, the O₂-tolerant [NiFeSe]-hydrogenase is able to efficiently operate in a H₂/air gas-diffusion enzymatic fuel cell