CHEMICAL CONSTITUENTS ISOLATED FROM Turnera subulata

Turnera subulata Sm., known as "Chanana" or "flor-do-Guarujá" in Brazilian folklore, is a plant species belonging to the subfamily Turneroideae of family Passifloraceae, which is used for various medicinal purposes in Brazil. The phytochemical study conducted here led to the isolation and identification of ten compounds present in T. subulata: two mixtures of steroids, sitosterol and stigmasterol (nonglycosylated and glycosylated); a mixture of flavonoids, 5,7,4′-trihidroxiflavona-8-C-α-glucopyranoside and 5,7,3′,4′-tetrahidroxiflavona-8-C-α-glucopyranosidel; and four phaeophytins, phaeophytin purpurin-18-phytyl ester, a rare natural product, phaeophytin a , 13²-hydroxy-(13²-S)-phaeophytin a , and phaeophytin b Phaeophytin b exhibited electrochemical activity similar to that of phthalocyanines

Chemical constituents from Sidastrum paniculatum and evaluation of their leishmanicidal activity

AbstractSidastrum paniculatum (L.) Fryxell, Malvaceae, is popularly known in Brazil as “malva-roxa” or “malvavisco”. The species is found mainly in Northeast region where it is used by locals to treat spider bites and bee stings. Aiming to identify the chemical compounds from S. paniculatum secondary metabolism and to contribute to the chemotaxonomic knowledge of Malvaceae family, a phytochemical study of S. paniculatum was carried out. Besides that, the isolated compounds were evaluated for antileishmanial activity against promastigotes of Leishmania braziliensis. By using chromatographic techniques the study resulted the isolation of eight compounds: 3-oxo-21β-H-hop-22(29)-ene; sebiferic acid; sitosterol 3-O-β-d-glucopyranoside/stigmasterol 3-O-β-d-glucopyranoside; phaeophytin a; 132(S)-hydroxyphaeophytin a; 132(S)-hydroxy-(173)-ethoxyphaeophorbide a and 7,4′-di-O-methylisoescutellarein. The structure of all isolated compounds was elucidated by spectroscopic analysis, including two-dimensional NMR techniques. In addition, the isolated compounds phaeophytin a; 132(S)-hydroxyphaeophytin a; 132(S)-hydroxy-(173)-ethoxyphaeophorbide a and 7,4′-di-O-methylisoescutellarein exhibited antileishmanial activity against promastigotes of L. braziliensis

New Alcamide and Anti-oxidant Activity of Pilosocereus gounellei A. Weber ex K. Schum. Bly. ex Rowl. (Cactaceae)

The Cactaceae family is composed by 124 genera and about 1438 species. Pilosocereus gounellei, popularly known in Brazil as xique-xique, is used in folk medicine to treat prostate inflammation, gastrointestinal and urinary diseases. The pioneering phytochemical study of P. gounellei was performed using column chromatography and HPLC, resulting in the isolation of 10 substances: pinostrobin (1), β-sitosterol (2), a mixture of sitosterol 3-O-β-d-glucopyranoside/stigmasterol 3-O-β-d-glucopyranoside (3a/3b), 132-hydroxyphaeophytin a (4), phaeophytin a (5), a mixture of β-sitosterol and stigmasterol (6a/6b), kaempferol (7), quercetin (8), 7′-ethoxy-trans-feruloyltyramine (mariannein, 9) and trans-feruloyl tyramine (10). Compound 9 is reported for the first time in the literature. The structural characterization of the compounds was performed by analyses of 1-D and 2-D NMR data. In addition, a phenolic and flavonol total content assay was carried out, and the anti-oxidant potential of P. gounellei was demonstrated

PHYTOCHEMISTRY INVESTIGATION OF CASEARIA ARBOREA (RICH.) URB. (SALICACEAE) AND ANTIMICROBIAL ANALYSIS OF ITS DITERPENE

A phytochemical investigation from aerial parts of Casearia arborea (Rich.) Urb. (Salicaceae) led to isolation and identification of: sitosterol, 4-en-stigmast-3-one, 13-hydroxy-trans-ent-cleroda-3,14-diene,3-hydroxy-2-oxo-trans-ent-cleroda-3,14-diene (kolavelone), a mixture of 13-hydroxy-trans-ent-cleroda-3,14-diene and an ester ethyl hexadecanoate, kaempferol-3-O-a-L-arabinofuranoside and 4',5,7-trihydroxy-3',5'-dimethoxyflavone (tricin). The compounds have been described for the first time in this species. Five trihydroxy-flavone-hexoside derivatives have been identified by LC-ESI-HR-MS. The antimicrobial activity of kolavelone was evaluated against strains of Staphylococcus, Bacillus, Pseudomonas, Shigella and Candida spores, and its minimum inhibitory concentration (MIC) was determined. The results showed the antimicrobial activity of kolavelone against several bacteria and Candida tropicalis indicating its potential use as antimicrobial agent

PHYTOCHEMISTRY INVESTIGATION OF CASEARIA ARBOREA (RICH.) URB. (SALICACEAE) AND ANTIMICROBIAL ANALYSIS OF ITS DITERPENE

<div><p>A phytochemical investigation from aerial parts of Casearia arborea (Rich.) Urb. (Salicaceae) led to isolation and identification of: sitosterol, 4-en-stigmast-3-one, 13-hydroxy-trans-ent-cleroda-3,14-diene,3-hydroxy-2-oxo-trans-ent-cleroda-3,14-diene (kolavelone), a mixture of 13-hydroxy-trans-ent-cleroda-3,14-diene and an ester ethyl hexadecanoate, kaempferol-3-O-a-L-arabinofuranoside and 4',5,7-trihydroxy-3',5'-dimethoxyflavone (tricin). The compounds have been described for the first time in this species. Five trihydroxy-flavone-hexoside derivatives have been identified by LC-ESI-HR-MS. The antimicrobial activity of kolavelone was evaluated against strains of Staphylococcus, Bacillus, Pseudomonas, Shigella and Candida spores, and its minimum inhibitory concentration (MIC) was determined. The results showed the antimicrobial activity of kolavelone against several bacteria and Candida tropicalis indicating its potential use as antimicrobial agent.</p></div