MANAGEMENT OF BUERGER'S DISEASE THROUGH AYURVEDA- A CASE REPORT

The present case report was about a 50 year-old-male who was diagnosed with Buerger’s disease which is a generalised occlusive vascular diseaseaffecting predominantly males with special predilection to involve the lowerextremities. He presented with complaint of unhealed ulcerations with pain andburning sensation in his right sole for 6 months but not getting satisfactoryrelief even after using conventional medications and advised to get doneamputation. He visited the All India institute of Ayurveda and was treated fortwo months with a combination of Ayurvedic medicines and leech therapy whichshowed significant improvement in patients condition and eventually norecurrence was observed, ulcer healed completely with slight discolouration. Thisshows the effectiveness of Ayurvedic treatment including internal medicinesadministration and leech therapy in preventing amputation and treating othersymptoms which may be useful for clinical practices

A comparative review of maternal and neonatal outcome among pregnant women with COVID-19 in first and second wave in a tertiary care centre of South Rajasthan

Background: Corona virus disease 2019 has taken a huge toll over health infrastructure and care all across the world. This article depicts our experience of COVID-19 in pregnant women and analyses maternal and neonatal outcome of same in first and second wave of this pandemic. Aim and objectives of the study were to compare the demographic characteristics, presenting features and fetomaternal outcome in COVID positive pregnant women in first v/s second wave in a tertiary care hospital.Methods: The RTPCR COVID-19 positive pregnant women admitted during the period April-2020 to March-2021 were considered in 1st wave of COVD-19 and that from April-2021 till June-2021 as 2nd wave of COVID-19. Data like baseline characteristics, past medical, obstetric history, clinical presentation, laboratory results, imaging findings, management modalities, maternal and neonatal outcome were analysed and compared.Results:  Peak of 1st wave of COVID-19 was found during the months of July-September 2020, while of 2nd in April-June 2021. Most women presented with COVID-19 RTPCR positive were asymptomatic both in 1st and 2nd wave. Though most patients were managed on room air in both waves, 6.52% and 9.38% were on oxygen, 1.09% and 10.94% were managed with mechanical ventilation and BIPAP in 1st and 2nd wave respectively. There was significant (p<0.05) increase in maternal deaths in the 2nd wave (7.03%) as compared to 1st wave (1.09%).Conclusions: A significantly large number of patients were affected in 2nd wave of COVID-19 pandemic with more morbidity and mortality. Neonatal population remained relatively unaffected in both waves

Id4 dependent acetylation restores mutant-p53 transcriptional activity

BACKGROUND: The mechanisms that can restore biological activity of mutant p53 are an area of high interest given that mutant p53 expression is observed in one third of prostate cancer. Here we demonstrate that Id4, an HLH transcriptional regulator and a tumor suppressor, can restore the mutant p53 transcriptional activity in prostate cancer cells. METHODS: Id4 was over-expressed in prostate cancer cell line DU145 harboring mutant p53 (P223L and V274F) and silenced in LNCaP cells with wild type p53. The cells were used to quantitate apoptosis, p53 localization, p53 DNA binding and transcriptional activity. Immuno-precipitation/-blot studies were performed to demonstrate interactions between Id4, p53 and CBP/p300 and acetylation of specific lysine residues within p53. RESULTS: Ectopic expression of Id4 in DU145 cells resulted in increased apoptosis and expression of BAX, PUMA and p21, the transcriptional targets of p53. Mutant p53 gained DNA binding and transcriptional activity in the presence of Id4 in DU145 cells. Conversely, loss of Id4 in LNCaP cells abrogated wild type p53 DNA binding and transactivation potential. Gain of Id4 resulted in increased acetylation of mutant p53 whereas loss of Id4 lead to decreased acetylation in DU145 and LNCaP cells respectively. Id4 dependent acetylation of p53 was in part due to a physical interaction between Id4, p53 and acetyl-transferase CBP/p300. CONCLUSIONS: Taken together, our results suggest that Id4 regulates the activity of wild type and mutant p53. Id4 promoted the assembly of a macromolecular complex involving CBP/P300 that resulted in acetylation of p53 at K373, a critical post-translational modification required for its biological activity

Id4 Deficiency Attenuates Prostate Development and Promotes PIN-like Lesions by Regulating Androgen Receptor Activity and Expression of NKX3.1 and PTEN

Background: Inhibitor of differentiation 4 (Id4), a member of the helix-loop-helix family of transcriptional regulators has emerged as a tumor suppressor in prostate cancer. Id4 is expressed in the normal prostate where its expression is also regulated by androgens. In this study we investigated the effect of loss of Id4 (Id4-/-) on adult prostate morphology. Methods: Histological analysis was performed on prostates from 6-8 weeks old Id4-/-, Id4+/- and Id4+/+ mice. Expression of Id1, Sox9, Myc, androgen receptor, Akt, p-Akt, Pten and Nkx3.1 was investigated by immunohistochemistry. Androgen receptor binding on NKX3.1 promoter was studied by chromatin immuno-precipitation. Id4 was either over-expressed or silenced in prostate cancer cell lines DU145 and LNCaP respectively followed by analysis of PTEN, NKX3.1 and Sox9 expression

Genetic variability and character association studies in bread wheat (Triticum aestivum L.) under two different water regimes

The present study was undertaken to estimate the GCV, PCV, heritability, genetic advance, and correlation for yield and its attributes in a set of forty-nine bread wheat genotypes, assessed under irrigated and rainfed conditions. Relatively high GCV, PCV, heritability, and genetic advance were found for TPP, GYP, and BYP in both conditions. It implying that crop improvement can be achieved by selecting these traits and such characters could be transmitted through hybridization to the progeny. In the correlation study, the highest significant positive correlation was observed between GPS and GWS in both environments. The results demonstrated that TPP and BYP are important components for improving grain yield in both irrigated and rainfed conditions

Altered Regulation of Aquaporin Gene Expression in Allergen and IL-13-Induced Mouse Models of Asthma

IL-13 is known to affect many processes that contribute to an asthmatic phenotype, including inflammation, fibrosis, and mucus production. Members of the aquaporin (AQP) family of transmembrane water channels are targets of regulation in models of lung injury and inflammation. Therefore, we examined AQP mRNA and protein expression in allergen and IL-13-induced mouse models of asthma. Lungs from ovalbumin sensitized and ovalbumin challenged (OVA/OVA) and IL-13 treated mice showed airway thickening, increased mucus production, and pulmonary eosinophilia. Pulmonary function tests showed a significant increase in methacholine-induced airway hyperreactivity in OVA/OVA and IL-13-treated mice as compared with controls. Quantitative PCR analysis revealed differential regulation of AQPs in these two models. AQP1 and AQP4 mRNA expression was downregulated in the OVA/OVA model, but not in the IL-13 model. AQP5 mRNA was reduced in both models, whereas AQP3 was upregulated only in the IL-13 model. Western analysis showed that diminished expression of an apically localized aquaporin, (AQP5), and concomitant upregulation of a basolateral aquaporin (AQP3 or AQP4) are characteristic features of both inducible asthma models. These results demonstrate that aquaporins are common targets of gene expression in both allergen and IL-13 induced mouse models of asthma

Evaluating air quality and criteria pollutants prediction disparities by data mining along a stretch of urban-rural agglomeration includes coal-mine belts and thermal power plants

Air pollution has become a threat to human life around the world since researchers have demonstrated several effects of air pollution to the environment, climate, and society. The proposed research was organized in terms of National Air Quality Index (NAQI) and air pollutants prediction using data mining algorithms for particular timeframe dataset (01 January 2019, to 01 June 2021) in the industrial eastern coastal state of India. Over half of the study period, concentrations of PM2.5, PM10 and CO were several times higher than the NAQI standard limit. NAQI, in terms of consistency and frequency analysis, revealed that moderate level (ranges 101–200) has the maximum frequency of occurrence (26–158 days), and consistency was 36%–73% throughout the study period. The satisfactory level NAQI (ranges 51–100) frequency occurrence was 4–43 days with a consistency of 13%–67%. Poor to very poor level of air quality was found 13–50 days of the year, with a consistency of 9%–25%. Random Forest (RF), Support Vector Machine (SVM), Bagged Multivariate Adaptive Regression Splines (MARS) and Bayesian Regularized Neural Networks (BRNN) are the data mining algorithms, that showed higher efficiency for the prediction of PM2.5, PM10, NO2 and SO2 except for CO and O3 at Talcher and CO at Brajrajnagar. The Root Mean Square Error (RMSE) between observed and predicted values of PM2.5 (ranges 12.40–17.90) and correlation coefficient (r) (ranges 0.83–0.92) for training and testing data indicate about slightly better prediction of PM2.5 by RF, SVM, bagged MARS, and BRNN models at Talcher in comparison to PM2.5 RMSE (ranges 13.06–21.66) and r (ranges 0.64–0.91) at Brajrajnagar. However, PM10 (RMSE: 25.80–43.41; r: 0.57–0.90), NO2 (RMSE: 3.00–4.95; r: 0.42–0.88) and SO2 (RMSE: 2.78–5.46; r: 0.31–0.88) at Brajrajnagar are better than PM10 (RMSE: 35.40–55.33; r: 0.68–0.91), NO2 (RMSE: 4.99–9.11; r: 0.48–0.92), and SO2 (RMSE: 4.91–9.47; r: 0.20–0.93) between observed and predicted values of training and testing data at Talcher using RF, SVM, bagged MARS and BRNN models, respectively. Taylor plots demonstrated that these algorithms showed promising accuracy for predicting air quality. The findings will help scientific community and policymakers to understand the distribution of air pollutants to strategize reduction in air pollution and enhance air quality in the study region

Role of CED-4 in the activation of CED-3

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62523/1/388728a0.pd

Role of Exosomes in Tumor Induced Neo-Angiogenesis

Exosomes are the nanovesicles, belonging to the type of extracellular vesicles (EVs), produced by normal as well as tumor cells and function as a mode in cell-to-cell communication. Tumor cells utilize various approach to communicate with neighboring cells for facilitating tumor invasion and progression, one of these approaches has been shown through the release of exosomes. Tumor-derived exosomes (TEX) have the ability to reprogram/modulate the activity of target cells due to their genetic and molecular cargo. Such exosomes target endothelial cells (among others) in the tumor microenvironment (TME) to promote angiogenesis which is an important element for solid tumor growth and metastasis. So, exosomes play a vital role in cancer invasiveness and progression by harboring various cargoes that could accelerate angiogenesis. Here first, we will present an overview of exosomes, their biology, and their role in different cancer models. Then, we will emphasis on exosomes derived from tumor cells as tumor angiogenesis mediators with a particular importance on the underlying mechanisms in various cancer origins. In the end, we will unveil the therapeutic potential of tumor derived exosomes as drug delivery vehicles against angiogenesis