Influence of low oxygen and high carbon dioxide on shredded Galega kale quality for development of modified atmosphere packages

Respiration rate, sensory attributes, colour alterations, and water, chlorophyll and ascorbic acid contents were monitored during storage of shredded Galega kale (Brassica oleracea var. acephala DC.) at 20 ◦C to define an adequate range of O2 and CO2 partial pressures for product preservation. Different low O2 and high CO2 atmospheres were tested. First, tolerance to low O2 partial pressures (1, 2, 3 or 21 kPa O2 with balance N2) was tested. Quality retention was improved as O2 partial pressure was reduced and there was no induction of anaerobic respiration. Then, tolerance to high CO2 partial pressures (0, 10, 15 or 20 kPa CO2 plus 21 kPa O2 and balance N2) was tested. The high CO2 partial pressures extended the shelf life of the shredded kale and no symptoms of CO2 injury were detected. Finally, combinations of low O2 and high CO2 (1 or 2 kPa O2 plus 15 or 20 kPa CO2, with balance N2, and an air control) were analysed. No differences were observed among the different gas combinations. An atmosphere of 1–2 kPa O2 plus 15–20 kPa CO2 and balance N2 extends the shelf life of shredded Galega kale to 4–5 days at 20◦C, compared with 2–3 days in air storage. Predictive models of chlorophyll a and b degradation as a function of time and gas composition were developed

Modelling O2 and CO2 exchange for development of perforation mediated modifed atmosphere packaging

Perforation-mediated modi®ed atmosphere packaging relies on the use of macro perforations or tubes to control the O2 and CO2 exchange and create the desired atmosphere inside an otherwise gas-tight package. In this work, the O2 and CO2 exchange through a single tube was studied. Di erent temperatures (5±20°C) and tube dimensions (length from 9 to 17 mm and diameter from 6 to 30 mm) were tested. O2 and CO2 mass transfer coe cients were determined according to a lumped mass transfer capacitance model that yielded a good description of the gas transfer. Temperature in the range tested did not show a signi®cant e ect on the mass transfer coe cients. A multiplicative non-linear equation was found to yield a good prediction of the dependence of the O2 mass transfer coe cient on tube diameter and length. The ratio between the CO2 and O2 mass transfer coe cients, an important parameter in the design of MAP, was 0.81 and none of the factors tested in¯uenced its value

Modelling respiration rate of shredded Galega kale for development of modified atmosphere packaging

The design of modified atmosphere packaging (MAP) for fresh-cut produce requires an adequate model for prediction of respiration rate as a function of both temperature and gas composition. In this work, the O2 consumption and CO2 production rates of shredded Galega kale were studied. The storage temperatures used were 1, 5, 10, 15 and 20 C. The atmospheres tested were all combinations of 1, 5 and 10% v/v O2 plus 0, 10 and 20% v/v CO2 with the balance being N2, as well as ambient air. Temperature was the variable with the greatest influence on respiration rate and the effect of gas composition increased with temperature. The dependence of respiration rate on gas composition was well described by a Michaelis–Menten type equation with uncompetitive CO2 inhibition. The respiratory quotient (RQ) was found to be constant for the range of temperatures and gas compositions tested and was equal to 0:93 0:01. The constants of the Michaelis–Menten equation increased exponentially with temperature. The change over time of respiration rate of leaves exposed to air at 20 C was also analysed. It was observed that respiration rate decreased with time and that the ratio between the respiration rate of shredded and intact leaves was approximately constant in the period tested and equal to 2.8. 2002 Elsevier Science Ltd. All rights reserved

Pd/Cu Site Interchange and Non-Fermi-Liquid Behavior in UCu_4Pd

X-ray-absorption fine-structure measurements of the local structure in UCu_4Pd are described which indicate a probable lattice-disorder origin for non-Fermi-liquid behavior in this material. Short Pd-Cu distances are observed, consistent with 24 +/- 3% of the Pd atoms occupying nominally Cu sites. A "Kondo disorder" model, based on the effect on the local Kondo temperature T_K of this interchange and some additional bond-length disorder, agrees quantitatively with previous experimental susceptibility data, and therefore also with specific heat and magnetic resonance experiments.Comment: 4 pages, 3 PostScript figures, to be published in PR

Ubiquitinylation of α-Synuclein by Carboxyl Terminus Hsp70-Interacting Protein (CHIP) Is Regulated by Bcl-2-Associated Athanogene 5 (BAG5)

Parkinson's disease (PD) is a common neurodegenerative condition in which abnormalities in protein homeostasis, or proteostasis, may lead to accumulation of the protein α-synuclein (α-syn). Mutations within or multiplications of the gene encoding α-syn are known to cause genetic forms of PD and polymorphisms in the gene are recently established risk factors for idiopathic PD. α-syn is a major component of Lewy bodies, the intracellular proteinaceous inclusions which are pathological hallmarks of most forms of PD. Recent evidence demonstrates that α-syn can self associate into soluble oligomeric species and implicates these α-syn oligomers in cell death. We have previously shown that carboxyl terminus of Hsp70-interacting protein (CHIP), a co-chaperone molecule with E3 ubiquitin ligase activity, may reduce the levels of toxic α-syn oligomers. Here we demonstrate that α-syn is ubiquitinylated by CHIP both in vitro and in cells. We find that the products from ubiquitinylation by CHIP include both monoubiquitinylated and polyubiquitinylated forms of α-syn. We also demonstrate that CHIP and α-syn exist within a protein complex with the co-chaperone bcl-2-associated athanogene 5 (BAG5) in brain. The interaction of CHIP with BAG5 is mediated by Hsp70 which binds to the tetratricopeptide repeat domain of CHIP and the BAG domains of BAG5. The Hsp70-mediated association of BAG5 with CHIP results in inhibition of CHIP E3 ubiquitin ligase activity and subsequently reduces α-syn ubiquitinylation. Furthermore, we use a luciferase-based protein-fragment complementation assay of α-syn oligomerization to investigate regulation of α-syn oligomers by CHIP in living cells. We demonstrate that BAG5 mitigates the ability of CHIP to reduce α-syn oligomerization and that non-ubiquitinylated α-syn has an increased propensity for oligomerization. Thus, our results identify CHIP as an E3 ubiquitin ligase of α-syn and suggest a novel function for BAG5 as a modulator of CHIP E3 ubiquitin ligase activity with implications for CHIP-mediated regulation of α-syn oligomerization

A Novel Role for Aquaporin-5 in Enhancing Microtubule Organization and Stability

Abstract Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. In addition to regulating transcellular water permeability, AQP5 can regulate paracellular permeability, though the mechanisms by which this occurs have not been determined. Microtubules also regulate paracellular permeability. Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells. Specifically, reduced levels of AQP5 correlated with lower levels of assembled microtubules and decreased paracellular permeability. In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function

Fermionic approach to the evaluation of integrals of rational symmetric functions

We use the fermionic construction of two-matrix model partition functions to evaluate integrals over rational symmetric functions. This approach is complementary to the one used in the paper ``Integrals of Rational Symmetric Functions, Two-Matrix Models and Biorthogonal Polynomials'' \cite{paper2}, where these integrals were evaluated by a direct method.Comment: 34 page

Integrability in SFT and new representation of KP tau-function

We are investigating the properties of vacuum and boundary states in the CFT of free bosons under the conformal transformation. We show that transformed vacuum (boundary state) is given in terms of tau-functions of dispersionless KP (Toda) hierarchies. Applications of this approach to string field theory is considered. We recognize in Neumann coefficients the matrix of second derivatives of tau-function of dispersionless KP and identify surface states with the conformally transformed vacuum of free field theory.Comment: 25 pp, LaTeX, reference added in the Section 3.