Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers

Background: The amphiploid species Brassica napus (oilseed rape, Canola) is a globally important oil crop yielding food, biofuels and industrial compounds such as lubricants and surfactants. Identification of the likely ancestors of each of the two genomes (designated A and C) found in B. napus would facilitate incorporation of novel alleles from the wider Brassica genepool in oilseed rape crop genetic improvement programmes. Knowledge of the closest extant relatives of the genotypes involved in the initial formation of B. napus would also allow further investigation of the genetic factors required for the formation of a stable amphiploid and permit the more efficient creation of fully fertile re-synthesised B. napus. We have used a combination of chloroplast and nuclear genetic markers to investigate the closest extant relatives of the original maternal progenitors of B. napus. This was based on a comprehensive sampling of the relevant genepools, including 83 accessions of A genome B. rapa L. (both wild and cultivated types), 94 accessions of B. napus and 181 accessions of C genome wild and cultivated B. oleracea L. and related species. Results: Three chloroplast haplotypes occurred in B. napus. The most prevalent haplotype (found in 79% of accessions) was not present within the C genome accessions but was found at low frequencies in B. rapa. Chloroplast haplotypes characteristic of B. napus were found in a small number of wild and weedy B. rapa populations, and also in two accessions of cultivated B. rapa 'brocoletto'. Whilst introgression of the B. napus chloroplast type in the wild and weedy B. rapa populations has been proposed by other studies, the presence of this haplotype within the two brocoletto accessions is unexplained. Conclusions: The distribution of chloroplast haplotypes eliminates any of the C genome species as being the maternal ancestor of the majority of the B. napus accessions. The presence of multiple chloroplast haplotypes in B. napus and B. rapa accessions was not correlated with nuclear genetic diversity as determined by AFLPs, indicating that such accessions do not represent recent hybrids. Whilst some chloroplast diversity observed within B. napus can be explained by introgression from inter-specific crosses made during crop improvement programmes, there is evidence that the original hybridisation event resulting in to B. napus occurred on more than one occasion, and involved different maternal genotypes

Social Organisation and the Rise of Civilisation in the Mun River Valley, Thailand

As one of the most extensively excavated and archaeologically interesting areas of Thailand, the Upper Mun River valley is central to archaeologists‘ interpretations of Southeast Asian prehistory. In this area there is demonstrable growth in social complexity from first occupation to eventual annexation by the state society of Angkor in the mid first millennium AD. The exact nature of social evolution, however, is still not fully understood. Debate rages over the factors upon which social stratification was based, whether hierarchy or heterarchy was in place, the timing of agricultural intensification and impact of external populations. In this thesis isotopic studies are combined with osteological indicators of kinship and population affinity to shed light on these and other archaeological problems which remain unanswered in the Upper Mun River Valley. Isotopic analysis has allowed the identification of migrants in the cemetery of Ban Non Wat, and shown changes in subsistence strategy through time relating to the onset of social inequality and climate change. Analysis of cranial shape has shown that most migrant individuals have similar genetic backgrounds to local people, but with the notable exception of one of the only adult jar burials at the site. The combination of dental non-metric techniques, isotopic analysis and cranial shape analysis has also added evidence to the debates over the presence of hunter-gatherers at the site, and the nature of social organization

Viability Tests of Seeds of Different Ages

Studies of longevity of seeds, of dormant period, and of vitality after subjection to certain conditions, have been made by numerous investigators, with many kinds of seeds. These all contribute to the understanding of changes which take place in the development of the seed, and of conditions necessary for growth. Such determinations have definite application to scientific agriculture. There may be called to notice results of a few notable studies of germination conditions of seeds. Dr. Candolle found certain seeds of Malvaceae, Leguminosae and Labiate to be germinable after having been kept dry for fifteen years. Beal found that various seeds, after having been buried for twenty-five years, grew; among these were Amaranthus retroflexus, Brassica nigra, Capsella Bursa-pastoris, Lepidium virginicum, Oenothera biennis, Polygonum Hydropiper, Portulaca olcracea, and Rumex crispus. Dr. L. H. Pammel determined, in experiments with a large number of kinds of weed seeds, that in general they germinate better after freezing and out-of-door conditions of wintering than when held over winter in dry storage

International Law - New Actors and New Technologies: Center Stage for NGOs

Technology and the information age are changing the allocation of power and authority in the international system with non-state actors such as intergovernmental organizations (IGOs) and nongovernmental organizations (NGOs) assuming decision-making roles previously reserved primarily to states. Professor David Johnston sees the information age as creating deep and broad disruptive breaches in our society, disruptions equal to those of the agricultural or industrial revolutions. Professors Keohane and Nye believe that the information age will alter the power structure of governments. Jessica Mathews\u27s stimulating article in Foreign Affairs argues both that the information revolution is shaking the foundations of state authority, the principal tenet of international law since 1648, and that the scholarly community has been slow to understand the profound ramifications of these changes. In presentations we made at the Fourth Joint Conference (American Society of International Law/ Nederlandse Vereniging voor Internationaal Recht) held in The Hague in 1997, we argued that the context within which international law operates has been shaped by two broad forces: (1) the state-centric character of the post-Westphalian international system; and (2) the Gutenberg global information system dominated by the printed word. The former has been analyzed extensively; the latter, at least so far as it affects international law, largely has been ignored. This Article focuses on NGOs to test their newly achieved prominence in international law-making by examining their role in the Landmines Convention and in the thwarting of the Multilateral Agreement on Investment. Are NGOs a manifestation of new governance structures emerging in the information age? Can they be a check against non-democratic, unaccountable, and aloof intergovernmental institutions that may complicate, rather than solve, problems?16 So that our discussion will be rooted in international law as usually understood, we examine both international law\u27s encounters with NGOs and how NGOs relate to the sources of international law

Choice of Language in Bilateral Treaties: Fifty Years of Changing State Practice

The language in which treaties are written affects how widely and deeply treaty obligations are understood and, hence, followed. Of course, many problems arise when the treaty does not have the same meaning in different languages. The focus of this article is a different aspect of language in treaties--the choice of language or languages as official text or texts of bilateral treaties. Some research has addressed the broader issue of multiple use of languages in international organizations and multilateral treaties, but bilateral treaties have received scant attention. This inattention likely stems from the difficulty of examining the treaty practice of more than 150 states in tens of thousands of treaties, a difficulty now largely overcome by modern database management techniques. This article examines a lengthy period of state practice, the half century between 1920 and 1970, in order to describe and understand language choices. At first blush, this may seem like much ado about nothing. Of course, it is the content, not the choice of official text, that matters most. However, the approach taken here, based as it is on an enormous amount of state practice, can elucidate a number of important issues: *To what degree has English taken over the lingua franca role previously played by Latin and French? *Is there any political dimension to language choice? *Has the rise of the United States and the Soviet Union as superpowers after World War II been accompanied by an increased use of English and Russian as official texts? *Is the emergence of the Third World as a major force in the international system reflected in languages used in treaties

Choice of Language in Bilateral Treaties: Fifty Years of Changing State Practice

The language in which treaties are written affects how widely and deeply treaty obligations are understood and, hence, followed. Of course, many problems arise when the treaty does not have the same meaning in different languages. The focus of this article is a different aspect of language in treaties--the choice of language or languages as official text or texts of bilateral treaties. Some research has addressed the broader issue of multiple use of languages in international organizations and multilateral treaties, but bilateral treaties have received scant attention. This inattention likely stems from the difficulty of examining the treaty practice of more than 150 states in tens of thousands of treaties, a difficulty now largely overcome by modern database management techniques. This article examines a lengthy period of state practice, the half century between 1920 and 1970, in order to describe and understand language choices. At first blush, this may seem like much ado about nothing. Of course, it is the content, not the choice of official text, that matters most. However, the approach taken here, based as it is on an enormous amount of state practice, can elucidate a number of important issues: *To what degree has English taken over the lingua franca role previously played by Latin and French? *Is there any political dimension to language choice? *Has the rise of the United States and the Soviet Union as superpowers after World War II been accompanied by an increased use of English and Russian as official texts? *Is the emergence of the Third World as a major force in the international system reflected in languages used in treaties

"It always comes down to money" : recent changes in service provision to disabled children, young people and their families in Scotland

This study follows on previous research published by Scotland’s Commissioner for Children and Young People which examined the use of eligibility criteria and assessment tools in services to disabled children and young people in Scottish local authorities (Lancaster 2012). The Stage One research also aimed to identify whether changing assessment procedures were affecting support to these young people. Based on local authority responses, the study found little evidence of tightening eligibility criteria, reduced levels of support or cutbacks in services. In 2013, the Commissioner’s Office funded further research to gather the views of voluntary sector providers and disabled children, young people and their families. The main aim of this study is to examine changes in the availability and accessibility of publicly funded services for these families over the past two years. This research covers local authority services, voluntary sector service providers, health services and professions allied to medicine. It includes children and young people aged 1-20 with a wide range of impairments including mental distress. The research is broadly based on a social model of disability while also taking account of the day-to-day implications of impairment and the significance of personal experience. The research used five different methods: • An on-line survey of voluntary sector providers: 53 valid responses were returned • 10 focus groups with parents, recruited through nine voluntary organisations and one local authority across Scotland. 56 parents/ carers took part • Five focus groups with young disabled people (aged 12- 20), recruited through voluntary organisations mostly in central Scotland. Eighteen took part • A one to one session with a young person (aged 17) who does not use speech • Three case studies, conducted by telephone interview, with parents and a service provider. The study received ethical approval from the University of Strathclyde Ethics Committee. Careful attention was paid to ethical issues throughout. Three limitations can be identified in this research. The young people’s groups did not include participants with as wide a range of abilities as we had anticipated; the parents in the focus groups and case studies were largely self-selecting and, for the most part, the views of service providers are not represented. However, local authorities’ views were presented in the Stage One research. Fifty-three voluntary service organisations, providing publicly funded services to families with disabled children, responded to the survey. Their range of characteristics indicates that they represent a reasonable cross-section of voluntary sector providers in this field. Seventy-nine per cent offered support, advice and information to parents and many also provided short breaks, leisure activities, education support or support for self-advocacy. Almost all (87%) of respondents had experienced a cut in funding or a change in allocation procedures by public funders which reduced their ability to support families. In some cases, this had led to reduced provision or the closure of some projects. About a third spent less on staff training compared to two years ago, while 19% were employing less qualified or experienced staff. Nine respondents had increased charges for their services. Only 27% of respondents said they had not experienced a cut in funding. Service providers were ambivalent about the impact of changes on their services. While 72% of respondents said that disabled children and their families get as good a service from their organisation now as they did two years ago, responses to other questions in the survey suggest that 81% of them think that there has been some deterioration in their provision. More specifically, compared to two years ago, 48% were limiting the number of people using their services, 47% were unable to offer the same level of support to new service users while 45% could not provide the same level of one-to-one support to users generally. About a third of voluntary service providers reported families had to wait longer for their services while a quarter had seen users withdrawn from their services by local authorities in favour of other forms of support. Nevertheless, many voluntary service providers are having difficulty coping with the level of demand for their services as their own budgets are frozen or reduced. They see the erosion of local authority services by funding cuts creating more demand for the voluntary sector. Voluntary providers are finding ways to maintain their services by reconfiguring services, dropping ‘niceties’ such as providing lunches at carers’ meetings, taking a ‘best value’ approach and seeking out new funding sources. But there is concern that maintaining a good quality service does not solve the problem of waiting lists and excluded families. Some respondents see the current cutbacks by local authorities as undermining developments in policy and practice made in recent years. The right to assessment of need may be being undermined in some local authorities. Although only two respondents mentioned self-directed support (SDS) as a current source of their funding, a number of comments indicated an expectation that SDS will be used as a further means to cut budgets. There is evidence of growing unmet need which is not recorded. Service providers are concerned about families being excluded from any funding or support. 32% of respondents have already experienced changes in local authority eligibility criteria affecting access to their services while 23% were aware of changes planned for next year. Many reported that support was now only funded for the most complex cases, when children were at risk or families in crisis. Service providers fear that cuts in public funding for services, coupled with the recent changes to welfare benefits, will increase the stress and isolation experienced by families and disabled children and the consequent risk of marital and family breakdown. Fifty-six parents/family carers took part in 10 focus group held across Scotland. Between them, these 47 women and nine men were looking after 61 disabled children and young people aged between 2 and 20. Parents in every group reported withdrawals of, and reductions in, the support they receive from a range of services - local authority social work and education departments, FE colleges, voluntary organisations, health services and professions allied to medicine - over the last two years. Many families did not have a social worker, some had never had one. Several had experienced their social worker being withdrawn during the last two years. It seems parents were generally not consulted about this and most were unhappy about it. A few had tried unsuccessfully to get a social worker and been told they did not need one or could not have one due to cutbacks or staff shortages. With some notable exceptions, most parents who did have a social worker received a low level of support, often having to ‘chase’ him/her. There was widespread satisfaction with the quality of short breaks services, with various schemes and units being praised. Parents generally wanted longer and/ or more frequent breaks. In some cases allocated hours had been withdrawn, reduced or failed to materialise. There was evidence of short breaks increasingly being used as a form of crisis intervention rather than a preventative service. Previous research has shown that disabled children and young people, like most young people, value opportunities to take part in social and recreational activities and make friends. A huge shortage of suitable social clubs and opportunities for young people was reported, the summer holidays being a particularly challenging time. Parents reported closure of holiday play schemes and a reduced number of hours for the young person to attend social clubs. There were concerns about staff not being trained to work with disabled children. In three areas, charges had been introduced for some social or play activities. Many positive comments were made about the schools the children and young people attended. However, parents also described reductions in the level of support and, in some cases, the quality of education available in some schools. In their view, reduced staffing levels were resulting in inadequate physical care, decreased learning support, less one-toone support including for some children assessed as needing it and a reluctance in some schools to develop Co-ordinated Support Plans. A minority of parents expressed concerns about the health and safety of their children. Three young people had been out of school for six or seven months without satisfactory alternative arrangements in place for their education. These situations were the culmination of complex and protracted difficulties but parents believed that insufficient training and experience among staff was a significant contributory factor. Parents reported reduced availability of occupational therapy, physiotherapy and speech and language therapy. Whereas therapists used to make routine ‘maintenance’ visits to children at school or at home, now they only came out in response to a specific problem or need. There were long waiting lists for appointments and for aids and equipment. Staff shortages were reported in CAMHS, a wheelchair and seating service and among nurses on children’s hospitals. A small number of parents reported an increase in services, sometimes due to an increase in their child’s challenging behaviour or following intervention by politicians they had contacted after experiencing long delays. Only a handful of parents had signed up for direct payments or (in three pilot areas) selfdirected support. While enjoying the flexibility and choice these brought, acting as an employer was felt to be demanding and ‘scary.’ The wider introduction of SDS, from April 2014, was widely seen as a money-saving exercise. Many services had long waiting lists, with some families also facing delays in securing an assessment of their child’s or their own needs. Parents attributed the bulk of changes they were experiencing to financial cutbacks. Increased demand and higher numbers of children being diagnosed on the autistic spectrum were additional factors. There was little evidence of parents being consulted about reductions in service provision and, when they were, parents generally felt their views had not been taken on board. Changes were often made without re-assessment or review of the child’s or family’s needs or, if re-assessments did take place, parents were not aware of it, although they and their children should be actively involved. Often parents were informed about changes by letter or telephone. While some professionals were singled out for high praise, many parents thought that staff, especially within local authorities, did not understand or listen to them. Most groups reported examples of insensitive comments or actions by professionals. Changes in service provision were often highly stressful for parents, sometimes causing or increasing anxiety, depression and relationship difficulties between partners. For children and young people, reductions in service provision variously led to disappointment, isolation, disrupted routines and, in a few cases, loss of skills. In some cases, stress caused by changes in support also affected siblings and grandparents. Although not directly asked about this, many parents raised the issue of poor information provision, adding that they generally found out about services from other parents and their own sleuthing efforts. Some had a view that, due to scarce resources, authorities withheld information or even gave out disinformation. There was great anxiety about the future, both in terms of further financial cutbacks and ‘welfare reform’ and the perceived ‘void’ of support and opportunities for young people when they leave school. Five focus groups were held with a total of 18 young people plus a one-to-one interview with a participant who used little speech. The age range was 12-20. Fifteen males and four females took part: the reason for the gender disparity is not fully explained by the higher ratio of disabled males to females. The participants were recruited through voluntary sector organisations including three catering for young people with learning disabilities. The young people’s views about services differed in tone and focus from parents’ accounts. Parents were generally responsible for arranging and liaising with services: young people had little direct involvement of that kind. They discussed their use of services within the wider context of their everyday lives. Most had experience of using a service whose input had come to an end. Some did not know the reason; others related it to their increasing age, changing needs or interests or other personal circumstances. A few reported that a service (such as speech and language therapy or physiotherapy) had been withdrawn which they felt they still needed. One young person identified financial constraints, shortage of social workers and greater priority being given to work with children as the reasons she had lost her social worker. In several cases, professionals had suggested finding a befriender for the young person but this had not materialised, apparently because alternative supports were identified or no befriender was currently available. The participants attended and enjoyed a wide range of social and recreational activities and had more to say about these than other services. There was some evidence that young people were offered more choices within these services than other forms of provision. At the same time, there were a couple of examples of individuals feeling less included. One young woman was not involved in swimming sessions with the social club she attended while another person was unable to meet up with friends outside school and service settings. The young people identified various services they used and staff they knew, generally expressing satisfaction with both. At the same time, there was often a sense of the young people being ‘provided’ with support, for example, through referral from other services. While they were involved in everyday choices about activities and entertainment, they seemed to have little say in more significant decisions about which services they used, why they used them or how they used them. With some exceptions, family members, particularly mothers, were identified as a key source of support in the everyday and an interface or mediator with services. Mothers were often described as the main decisionmakers when it came to using services and the young people expressed confidence in the decisions taken. Other participants reported that professionals made decisions about the support they should have: they did not mention being consulted. One view was that, while professionals seek young people’s views about topics which they (the professionals) considered important, they do not ask young people what matters to them. Loss of certain supports was an issue for some older participants no longer eligible for children’s services. Some also expressed wider concerns about the move to adult life, including insufficient careers advice, support to prepare for job interviews and difficulties learning to drive. One young person felt she was being discriminated against at college while another believed that young disabled people face discrimination in the labour market. Three case studies were carried out focusing on two boys and a girl aged 7, 10 and 15 respectively. The three young people all had complex needs including challenging behaviours. Their mothers each took part in a telephone interview and were invited to nominate a service provider whom we could also interview. This led to one voluntary service provider taking part. The parents gave detailed accounts of mostly unwelcome changes made to their service provision over the preceding two years. Although all had their own experiences, some common themes emerged. All had experienced withdrawal, reduction or breakdown of services in the last two years, in one case with no alternative being offered, in others, with what parents saw as inadequate or inappropriate alternatives offered. In two cases, the substitute services proposed did not match assessed need. One family had been offered three alternative services; one never materialised and the other two were not available. Two parents stated there had been no review or re-assessment of need prior to the loss of service or subsequently. All three had been involved in protracted negotiations (between one and two years) with the local authority to secure better support. While some professionals were seen as trying to be helpful, others were not. Each parent had a sense of ‘changing goalposts’ in the local authority, two believing they had been deliberately misinformed on some matters. In all three cases, there was a lack of transparency in the way decisions to change or reduce services were made. Two had reached Stage 31 in the complaints procedure. The absence of adequate support, coupled with the young people’s challenging behaviour, placed huge stress on families, including siblings. The voluntary service provider interviewed, whose input to the family had not changed, acknowledged the increased stress to the family and empathised with their frustration. Equally, she understood the limited resources available to the local authority and questioned its ability to provide personalised support to young people with complex needs. While some findings from this study coincide with local authorities’ accounts in the Stage One research, significant differences have also emerged. There is evidence of reductions in local authority budgets and services for disabled children, tightening eligibility criteria, support being removed without review or reassessment, and a lack of consultation with disabled children and young people. There is a real danger that children and young people’s entitlements under international conventions and UK and Scots law are being and will continue to be eroded, alongside the undermining of established good policy and practice. Reduced levels and quality of support and widespread deterioration in various aspects of service provision have led to less choice, long waiting lists and increased unmet need, with a shift away from preventative work to crisis intervention. There has been a small increase in charging for services, both in terms of increased rates and introduction of new charges. The uptake of direct payments and SDS by these parents, on behalf of their children, was low. There were mixed feelings about their benefits. The wider implementation of SDS from April 2014 was widely viewed as a money saving exercise. There was a widespread view that disabled children, young people and their families were being ‘discriminated against by services’ and ‘treated like second class citizens’. Next steps - proposed actions for public bodies Local authorities, health boards and voluntary organisations must ensure they are observing disabled children’s legislative rights and entitlements. Specifically: • Under the Children (Scotland) Act 1995, local authorities must consult with children and young people, using accessible formats, and take their views into account when making decisions. Parents also have a right to be consulted. • Under the same Act, local authorities must formally assess a child’s needs when a parent asks them to do so. • If a child is assessed as needing certain named services, such as aids and equipment, practical help in the home, travel or recreational facilities, and is eligible for them, then under the Chronically Sick and Disabled Persons (Scotland) Act 1972, the local authority must provide them. • Local authorities should be aware that it is not good practice, and a previous judicial review2 shows it can be unlawful, to reduce or withdraw services from disabled children or young people without proper re-assessment or review of their needs. • Under the Children (Scotland) Act 1995, public bodies should publish information about available services: it would be helpful if practitioners actively disseminated such information, explaining how it applies to individual children. Accessible materials should be also available for children and young people In relation to specific issues: • Local authorities and health boards should ensure that budgets and staffing levels for disabled children’s services are sufficient to meet assessed need as well as the increasing number of service users and complexity of some cases. • Waiting lists should be actively managed and regularly monitored, with families being kept informed of progress and offered advice and information as appropriate. • Local authorities should inform disabled young people and their families about the benefits and the underlying principles of SDS and ensure practical assistance with the organisation and administration of direct payments is available. In relation to specific services: • There is a need for far more social and recreational opportunities for disabled children and young people, including those with life-limiting conditions. Local area co-ordinators, who have a capacity building remit, could support mainstream organisations to include disabled children and young people. • Professions allied to medicine (specifically, occupational theory, physiotherapy and speech and language therapy) should be more readily available to those disabled children and young people who would benefit from treatment on an on-going basis. • Local authorities should have arrangements in place for emergency care of disabled children and young people w

Results of seed investigations for 1908 and 1909

Since the publication of bulletins 88 and 99 on the subject of seed investigation, the Botanical Section has continued a study of the seeds offered for sale to the farmers of Iowa. Bulletin 88 contains the studies for 1906, bulletin 99 the studies for 1907, and the present publication contains the results of our work for 1908 and 1909. Since the operation of the Iowa law it has been our experience that the quality of the seed sold to the farmers is much better than formerly. Hundreds of samples sent to us by the farmers of this state for analysis indicate that they desire to protect themselves from the introduction of noxious weeds, especially dodder, quack grass, buckhorn, dock, and Canada thistle. The farmers have also learned that it is far better to buy a better quality of seed, seed that does not contain these noxious weeds, than that containing a goodly number of weed seeds that would prove harmful to agriculture

Delayed Germination

In 1901 there was begun a study of the germination of weed seeds under different conditions. It was observed that a large number of the weed seeds did not germinate freely in the fall