Improvement of smallholder farming systems in Africa

This forum paper provides a synthesis and discussion of 14 categories of lessons learned from experiences for achieving farm-level impact with smallholder farm- ers in Africa. These lessons were reported in a symposium hosted by the Agron- omy in Africa community of the American Society of Agronomy. The lessons, listed in order of frequency of reporting, were the need to: have adequate infras- tructure and services; enable spontaneous adoption; have multi-disciplinary and institutional collaboration; build on previous adoption of good agronomic practices (GAP); have farmer participation in research; encourage and learn from smallholder adaptations; make GAP promotion demand-driven; allow GAP choices; address challenges and trade-offs to GAP adoption; enable GAP-by-GAP adoption; reconcile conflicting messages; offer adequate profit potential with acceptable risk; reduce labor needs, especially for women; and build capacity for farming system improvement along the chain from farmer to research. The lessons are discussed and conclusions are reported

Influence of Land Use and Topographic Factors on Soil Organic Carbon Stocks and Their Spatial and Vertical Distribution

Soil organic carbon (SOC) plays a critical role in major ecosystem processes, agriculture, and climate mitigation. Accurate SOC predictions are challenging due to natural variation, as well as variation in data sources, sampling design, and modeling approaches. The goal of this study was to (i) understand SOC stock distribution due to land use (restored prairie grass—PG; lawn grass—LG; and forest—F), and local topography, and (ii) assess the scalability of SOC stock predictions from the study site in North Carolina (Lat: 36°7′ N; Longitude: 80°16′ W) to the geographic extension of the Fairview soil series based on the US Soil Survey Geographic (gSSURGO) database. Overall, LG had the highest SOC stock (82 Mg ha−1) followed by PG (79 Mg ha−1) and forest (73.1 Mg ha−1). SOC stock decreased with the depth for LG and PG, which had about 60% concentrated on the surface horizon (0–23 cm), while forest had only 40%. The differences between measured SOC stocks and those estimated by gSSURGO and modeled based on land use for the Fairview series extent were comparable. However, subtracting maps of the uncertainty predictions based on the 90% confidence interval (CI) derived from the measured values and estimated gSSURGO upper and lower values (an estimated CI) resulted in a range from −17 to 41 Mg ha−1 which, when valued monetarily, varied from USD 33 million to USD 824 million for the Fairview soil series extent. In addition, the spatial differences found by subtracting the gSSURGO estimations from measured uncertainties aligned with the county administrative boundaries. The distribution of SOC stock was found to be related to land use, topography, and soil depth, while accuracy predictions were also influenced by data source

Pre-treatment drug resistance and HIV-1 genetic diversity in the rural and urban settings of Northwest-Cameroon.

BackgroundWith the scale-up of antiretroviral therapy (ART), pre-treatment drug resistance (PDR) appears ≥10% amongst ART-initiators in many developing countries, including Cameroon. Northwest region-Cameroon having the second epidemiological burden of HIV infection, generating data on PDR in these geographical settings, will enhance evidence-based decision-making.ObjectivesWe sought to ascertain levels of PDR and HIV-1 clade dispersal in rural and urban settings, and their potential association with subtype distribution and CD4-staging.MethodsA cross-sectional study was conducted from February to May 2017 among patients recently diagnosed with HIV-infection and initiating ART at the Bamenda regional Hospital (urban setting) and the Mbingo Baptist hospital (rural setting). Protease and reverse transcriptase sequencing was performed using an in-house protocol and pre-treatment drug resistance mutations were interpreted using Stanford HIVdb.v8.3. Phylogeny was performed for subtype assignation.ResultsA total of 61 patient sequences were generated from ART initiators (median age: 37 years old; 57.4% female; median CD4 cell count: 184 [IQR: 35-387] in urban vs. 161 [IQR: 96-322] cells/mm3 in rural). Overall, the level of PDR was 9.8% (6/61). Of note, burden of PDR was almost doubled in urban (12.9% [4/31]) compared to rural setting 6.7% (2/30), p = 0.352). Fifteen (15) PDR mutations were found among four patients the urban settings [6 resistance mutations to NRTIs:[M41L (2), E44D (1), K65R (1), K70E (1), M184V/I (2), K219R (1)] and 6 resistance mutations to NNRTIs: K103N (1), E138A/G (2), V179E (1), M230L (1), K238T (1), P225H (1)] against two (02) mutations found in two patients in the rural setting[2 resistant mutations to NNRTIs: E138A (1) and Y188H (1)]. The rural setting showed more genetic diversity (8 subtypes) than the urban setting (5 subtypes), with CRF02_AG being the most prevalent clade (72.1% [44/61]). Of note, level of PDR was similar between patients infected with CRF02_AG and non-CRF02_AG infected (9.1% [4/44]) vs. 11.8% [2/17]), p = 1.000). Moreover, PDR appeared higher in patients with CD4 cell count ConclusionsPDR is at a moderate rate in the Northwest region of Cameroon, with higher burden within urban populations. CRF02_AG is the most predominant clade in both urban and rural settings. No effect of HIV molecular epidemiology and CD4-staging on the presence of PDR in patients living in these settings was found. Our findings suggest close monitoring, NNRTI-sparing regimens or sequencing for patients initiating ART, especially in urban settings