Transplantation of Human Neuroblastoma Cells, Catecholaminergic and Non-Catecholaminergic: Effects on Rotational Behavoir in Parkinson's Rat Model

Cultured human catecholaminergic and noncatecholaminergic donor cells were used in neural transplantation experiments in a rat model of Parkinson's disease. Using two different human catecholaminergic neuroblastoma cell lines, one control non-catecholaminergic neuroblastoma cell line, and one sham control (tissue culture medium), transplants were made into the striatum using a modified Ungerstedt hemiparkinsonian rat model. Significant decreases in apomorphine-induced rotational behavior were produced by two of three catecholaminergic cell lines. Grafted cells staining positively for tyrosine hydroxylase (TH) and catecholamine fluorescence indicated viable catecholamine activity in the two cell lines which produced reductions in rotational behavior. Catecholamine fluorescence was not detected in either of the two controls. These data suggest a link between catecholamine secretion by transplanted cells and motor improvement using a rat rotational behavior model

A Phase III Randomized Trial of Gemcitabine–Oxaliplatin versus Carboplatin–Paclitaxel as First-Line Therapy in Patients with Advanced Non-small Cell Lung Cancer

Purpose:This phase III study compared the efficacy and tolerability of gemcitabine and oxaliplatin (GEMOX) with paclitaxel and carboplatin (PCb) in chemotherapy-naive patients with stage IIIB/IV non-small cell lung cancer.Patients and Methods:Patients aged 18 years or older were randomized to PCb (paclitaxel 225 mg/m2 followed by carboplatin area under the curve = 6 on day 1 every 3 weeks) or GEMOX (gemcitabine 1,000 mg/m2 on days 1 and 8 followed by oxaliplatin 130 mg/m2 on day 1 every 3 weeks) for up to six cycles. The primary end point was progression-free survival (PFS), with tumor response rate, overall survival (OS), and quality of life as secondary end points.Results:The study was terminated after 383 patients had been randomized (371 received treatment) as the incidence of adverse events had exceeded the protocol-specified safety threshold (≥20% in either arm). No formal statistical comparisons were conducted. Median PFS was 4.44 months and 4.67 months in the GEMOX and PCb groups, respectively. Objective response rates (complete or partial) were 15.2% and 22.4% in the GEMOX and PCb arms, respectively. Median OS was 9.90 months (GEMOX) and 9.24 months (PCb); post hoc analyses showed median OS in patients aged 70 years or older to be similar to those younger than 70 years. PFS was similar in both groups of patients with adenocarcinoma histology, although OS favored the GEMOX group. Quality of life was improved from baseline in both groups. Toxicity profiles were comparable between the groups.Conclusion:PFS, OS, and objective response rates with GEMOX were similar to PCb. Nevertheless, toxicities limit the adoption of this regimen for routine use in advanced non-small cell lung cancer

Suzaku Observations of Local Ultraluminous Infrared Galaxies

We report the results from our analysis of {\it Suzaku} XIS (0.5-10 keV) and HXD/PIN (15-40 keV) observations of five well-known local ULIRGs: {\em IRAS} F05189-2524, {\em IRAS} F08572+3915, Mrk 273, PKS 1345+12, and Arp 220. The XIS observations of F05189-2524 and Mrk 273 reveal strong iron lines consistent with Fe K$\alpha$ and changes in spectral shapes with respect to previous {\it Chandra} and {\it XMM-Newton} observations. Mrk 273 is also detected by the HXD/PIN at $\sim$1.8-$\sigma$. For F05189-2524, modeling of the data from the different epochs suggests that the change in spectral shape is likely due to the central source switching off, leaving behind a residual reflection spectrum, or an increase in the absorbing column. An increase in the covering fraction of the absorber can describe the spectral variations seen in Mrk 273, although a reduction in the intrinsic AGN luminosity cannot be formally ruled out. The {\it Suzaku} spectra of Mrk 273 are well fit by a ~94% covering fraction model with a column density of $\sim10^{24}$ cm$^{-2}$. The absorption-corrected log[$L_{\rm 2-10 keV}$ / $L_{\rm IR}$] ratio is consistent with those found in PG Quasars. The 0.5-10 keV spectrum of PKS 1345+12 and Arp 220 seem unchanged from previous observations and their hard X-ray emission is not convincingly detected by the HXD/PIN. The large column density derived from CO observations and the large equivalent width of an ionized Fe line in Arp 220 can be reconciled by an ionized reflection model. F08572+3915 is undetected in both the XIS and HXD/PIN, but the analysis of unpublished {\em Chandra} data provides a new measurement at low energies.Comment: 37 pages including 4 tables and 10 figures. Accepted for publication in ApJ. It is tentatively scheduled to appear in the January 20, 2009 issue of Ap

Economic inequalities in the effectiveness of a primary care intervention for depression and suicidal ideation.

BACKGROUND: Economic disadvantage is associated with depression and suicide. We sought to determine whether economic disadvantage reduces the effectiveness of depression treatments received in primary care. METHODS: We conducted differential-effects analyses of the Prevention of Suicide in Primary Care Elderly: Collaborative Trial, a primary-care-based randomized, controlled trial for late-life depression and suicidal ideation conducted between 1999 and 2001, which included 514 patients with major depression or clinically significant minor depression. RESULTS: The intervention effect, defined as change in depressive symptoms from baseline, was stronger among persons reporting financial strain at baseline (differential effect size = -4.5 Hamilton Depression Rating Scale points across the study period [95% confidence interval = -8.6 to -0.3]). We found similar evidence for effect modification by neighborhood poverty, although the intervention effect weakened after the initial 4 months of the trial for participants residing in poor neighborhoods. There was no evidence of substantial differences in the effectiveness of the intervention on suicidal ideation and depression remission by economic disadvantage. CONCLUSIONS: Economic conditions moderated the effectiveness of primary-care-based treatment for late-life depression. Financially strained individuals benefited more from the intervention; we speculate this was because of the enhanced treatment management protocol, which led to a greater improvement in the care received by these persons. People living in poor neighborhoods experienced only temporary benefit from the intervention. Thus, multiple aspects of economic disadvantage affect depression treatment outcomes; additional work is needed to understand the underlying mechanisms

Simultaneous Chandra and RXTE Spectroscopy of the Microquasar H~1743-322: Clues to Disk Wind and Jet Formation from a Variable Ionized Outflow

We observed the bright phase of the 2003 outburst of the Galactic black hole candidate H 1743-322 in X-rays simultaneously with Chandra and RXTE on four occasions. The Chandra/HETGS spectra reveal narrow, variable (He-like) Fe XXV and (H-like) Fe XXVI resonance absorption lines. In the first observation, the Fe XXVI line has a FWHM of 1800 +/- 400 km/s and a blue-shift of 700 +/- 200 km/s, suggesting that the highly ionized medium is an outflow. Moreover, the Fe XXV line is observed to vary significantly on a timescale of a few hundred seconds in the first observation, which corresponds to the Keplerian orbital period at approximately 1 E+4 gravitational radii. Our models for the absorption geometry suggest that a combination of geometric effects and changing ionizing flux are required to account for the large changes in line flux observed between observations, and that the absorption likely occurs at a radius less than 1 E+4 radii for a 10 Msun black hole. Viable models for the absorption geometry include cyclic absorption due to an accretion disk structure, absorption in a clumpy outflowing disk wind, or possibly a combination of these two. If the wind in H 1743-322 has unity filling factor, the highest implied mass outflow rate is 20 percent of the Eddington mass accretion rate. This wind may be a hot precursor to the Seyfert-like, outflowing "warm absorber" geometries recently found in the Galactic black holes GX 339-4 and XTE J1650-500. We discuss these findings in the context of ionized Fe absorption lines found in the spectra of other Galactic sources, and connections to warm absorbers, winds, and jets in other accreting systems.Comment: 18 pages, 7 figures, 5 in color, subm. to ApJ. Uses emulateapj.sty and apjfonts.st

Evidence for Black Hole Spin in GX 339-4: XMM-Newton EPIC-pn and RXTE Spectroscopy of the Very High State

We have analyzed spectra of the Galactic black hole GX 339-4 obtained through simultaneous 76 ksec XMM-Newton/EPIC-pn and 10 ksec RXTE observations during a bright phase of its 2002-2003 outburst. An extremely skewed, relativistic Fe K-alpha emission line and ionized disk reflection spectrum are revealed in these spectra. Self-consistent models for the Fe K-alpha emission line profile and disk reflection spectrum rule-out an inner disk radius compatible with a Schwarzschild black hole at more than the 8 sigma level of confidence. The best-fit inner disk radius of 2-3 r_g suggests that GX 339-4 harbors a black hole with a ~ 0.8-0.9 (where r_g = GM/c^2 and a=cJ/GM^2, and assuming that reflection in the plunging region is relatively small). This confirms indications for black hole spin based on a Chandra spectrum obtained later in the outburst. The emission line and reflection spectrum also rule-out a standard power-law disk emissivity in GX 339-4; a broken power-law form with enhanced emissivity inside ~6 r_{g} gives improved fits at more than the 8 sigma level of confidence. The extreme red wing of the line and steep emissivity require a centrally--concentrated source of hard X-rays which can strongly illuminate the inner disk. Hard X-ray emission from the base of a jet - enhanced by gravitational light bending effects - could create the concentrated hard X-ray emission; this process may be related to magnetic connections between the black hole and the inner disk. We discuss these results within the context of recent results from analyses of XTE J1650-500 and MCG-6-30-15, and models for the inner accretion flow environment around black holes.Comment: 5 pages, 2 color figures, uses emulateapj.sty and apjfonts.sty, ApJ Letters, accepte

Modeling the role of fomites in a norovirus outbreak

Norovirus accounts for a large portion of the gastroenteritis disease burden, and outbreaks have occurred in a wide variety of environments. Understanding the role of fomites in norovirus transmission will inform behavioral interventions, such as hand washing and surface disinfection. The purpose of this study was to estimate the contribution of fomite-mediated exposures to infection and illness risks in outbreaks. A simulation model in discrete time that accounted for hand-to-porous surfaces, hand-to-nonporous surfaces, hand-to-mouth, -eyes, -nose, and hand washing events was used to predict 17 hr of simulated human behavior. Norovirus concentrations originated from monitoring contamination levels on surfaces during an outbreak on houseboats. To predict infection risk, two dose-response models (fractional Poisson and 2F1 hypergeometric) were used to capture a range of infection risks. A triangular distribution describing the conditional probability of illness given an infection was multiplied by modeled infection risks to estimate illness risks. Infection risks ranged from 70.22% to 72.20% and illness risks ranged from 21.29% to 70.36%. A sensitivity analysis revealed that the number of hand-to-mouth contacts and the number of hand washing events had strong relationships with model-predicted doses. Predicted illness risks overlapped with leisure setting and environmental attack rates reported in the literature. In the outbreak associated with the viral concentrations used in this study, attack rates ranged from 50% to 86%. This model suggests that fomites may have accounted for 25% to 82% of illnesses in this outbreak. Fomite-mediated exposures may contribute to a large portion of total attack rates in outbreaks involving multiple transmission modes. The findings of this study reinforce the importance of frequent fomite cleaning and hand washing, especially when ill persons are present.12 month embargo; published online: 04 Feb 2019This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]