Mise en place d'une expérience avec le grand public: entre recherche, vulgarisation et pédagogie

Methodological considerations on implementing a participative experimentWe present the implementation of an economic experiment conducted simultaneously in 11 French cities, with over 2700 participants, during four uninterrupted hours, during a popular-science event held in September 2015. Our goal is both to provide a roadmap for a possible replication and to discuss how the discipline can be used in new fields (science popularization, popular education, public communication)

Functional Interaction between CFTR and the Sodium-Phosphate Co-Transport Type 2a in Xenopus laevis Oocytes

A growing number of proteins, including ion transporters, have been shown to interact with Cystic Fibrosis Transmembrane conductance Regulator (CFTR). CFTR is an epithelial chloride channel that is involved in Cystic Fibrosis (CF) when mutated; thus a better knowledge of its functional interactome may help to understand the pathophysiology of this complex disease. In the present study, we investigated if CFTR and the sodium-phosphate co-transporter type 2a (NPT2a) functionally interact after heterologous expression of both proteins in Xenopus laevis oocytes.NPT2a was expressed alone or in combination with CFTR in X. laevis oocytes. Using the two-electrode voltage-clamp technique, the inorganic phosphate-induced current (IPi) was measured and taken as an index of NPT2a activity. The maximal IPi for NPT2a substrates was reduced when CFTR was co-expressed with NPT2a, suggesting a decrease in its expression at the oolemna. This was consistent with Western blot analysis showing reduced NPT2a plasma membrane expression in oocytes co-expressing both proteins, whereas NPT2a protein level in total cell lysate was the same in NPT2a- and NPT2a+CFTR-oocytes. In NPT2a+CFTR- but not in NPT2a-oocytes, IPi and NPT2a surface expression were increased upon PKA stimulation, whereas stimulation of Exchange Protein directly Activated by cAMP (EPAC) had no effect. When NPT2a-oocytes were injected with NEG2, a short amino-acid sequence from the CFTR regulatory domain that regulates PKA-dependent CFTR trafficking to the plasma membrane, IPi values and NPT2a membrane expression were diminished, and could be enhanced by PKA stimulation, thereby mimicking the effects of CFTR co-expression.We conclude that when both CFTR and NPT2a are expressed in X. laevis oocytes, CFTR confers to NPT2a a cAMPi-dependent trafficking to the membrane. This functional interaction raises the hypothesis that CFTR may play a role in phosphate homeostasis

Changes in the spatial distribution of erosion within a selectively logged rainforest catchment in Borneo 1988-2003

[No abstract available

Impact flux on Jupiter: From superbolides to large-scale collisions

Context. Regular observations of Jupiter by a large number of amateur astronomers have resulted in the serendipitous discovery of short bright flashes in its atmosphere, which have been proposed as being caused by impacts of small objects. Three flashes were detected: one on June 3, 2010, one on August 20, 2010, and one on September 10, 2012. Aims. We show that the flashes are caused by impacting objects that we characterize in terms of their size, and we study the flux of small impacts on Jupiter. Methods. We measured the light curves of these atmospheric airbursts to extract their luminous energy and computed the masses and sizes of the objects. We ran simulations of impacts and compared them with the light curves. We analyzed the statistical significance of these events in the large pool of Jupiter observations. Results. All three objects are in the 5-20 m size category depending on their density, and they released energy comparable to the recent Chelyabinsk airburst. Model simulations approximately agree with the interpretation of the limited observations. Biases in observations of Jupiter suggest a rate of 12-60 similar impacts per year and we provide software tools for amateurs to examine the faint signature of impacts in their data to increase the number of detected collisions. Conclusions. The impact rate agrees with dynamical models of comets. More massive objects (a few 100 m) should impact with Jupiter every few years leaving atmospheric dark debris features that could be detectable about once per decade. © ESO, 2013