A memória da luz : customizações e encontros com o espectador

Tese (doutorado)—Universidade de Brasília, Instituto de Artes, Departamento de Artes Visuais, 2017.O presente trabalho fala do desenvolvimento e aplicação de simulacros computacionais da luz como opção poética que é estética e empiricamente investigada de modo a envolver a comparação de minhas percepções e meus horizontes, como artista, com os de meus espectadores, como forma de encontrar aproximações e distâncias entre essas percepções e horizontes enquanto criações necessariamente implicadas na fruição esteticamente qualificada desses simulacros, em favor de um maior entendimento sobre o que poderia ser um encontro ou comunicação possível entre autor e receptor e na expectativa de obter, pelo somatório de percepções e horizontes colhidos desses espectadores, uma revelação paulatina das propostas artísticas correspondentes a esses simulacros.This work is about the development and application of computer light simulacra as poetic option that is aesthetically and empirically investigated in a way that involves the comparison of my perceptions and horizons, as an artist, with those of my spectators, as a way to find the proximities and distances between those perceptions and horizons as creations necessarily implied in the fruition aesthetically qualified of these simulacra, in favor of a greater understanding of what could be a possible encounter or communication between author and receiver and in the expectation of a gradual revelation of the artistic proposals corresponding to these simulacra, by the sum of perceptions and horizons taken from those spectators

Dynamics Determine Signaling in a Multicomponent System Associated with Rheumatoid Arthritis

Strategies that target multiple components are usually required for treatment of diseases originating from complex biological systems. The multicomponent system consisting of the DR4 major histocompatibility complex type II molecule, the glycopeptide CII259–273 from type II collagen, and a T-cell receptor is associated with development of rheumatoid arthritis (RA). We introduced non-native amino acids and amide bond isosteres into CII259–273 and investigated the effect on binding to DR4 and the subsequent T-cell response. Molecular dynamics simulations revealed that complexes between DR4 and derivatives of CII259–273 were highly dynamic. Signaling in the overall multicomponent system was found to depend on formation of an appropriate number of dynamic intramolecular hydrogen bonds between DR4 and CII259–273, together with the positioning of the galactose moiety of CII259–273 in the DR4 binding groove. Interestingly, the system tolerated modifications at several positions in CII259–273, indicating opportunities to use analogues to increase our understanding of how rheumatoid arthritis develops and for evaluation as vaccines to treat RA

Dynamics Determine Signaling in a Multicomponent System Associated with Rheumatoid Arthritis

Strategies that target multiple components are usually required for treatment of diseases originating from complex biological systems. The multicomponent system consisting of the DR4 major histocompatibility complex type II molecule, the glycopeptide CII259–273 from type II collagen, and a T-cell receptor is associated with development of rheumatoid arthritis (RA). We introduced non-native amino acids and amide bond isosteres into CII259–273 and investigated the effect on binding to DR4 and the subsequent T-cell response. Molecular dynamics simulations revealed that complexes between DR4 and derivatives of CII259–273 were highly dynamic. Signaling in the overall multicomponent system was found to depend on formation of an appropriate number of dynamic intramolecular hydrogen bonds between DR4 and CII259–273, together with the positioning of the galactose moiety of CII259–273 in the DR4 binding groove. Interestingly, the system tolerated modifications at several positions in CII259–273, indicating opportunities to use analogues to increase our understanding of how rheumatoid arthritis develops and for evaluation as vaccines to treat RA

Anionic Lipid Binding to the Foreign Protein MGS Provides a Tight Coupling between Phospholipid Synthesis and Protein Overexpression in <i>Escherichia coli</i>

Certain membrane proteins involved in lipid synthesis can induce formation of new intracellular membranes in <i>Escherichia coli</i>, i.e., intracellular vesicles. Among those, the foreign monotopic glycosyltransferase MGS from <i>Acholeplasma laidlawii</i> triggers such massive lipid synthesis when overexpressed. To examine the mechanism behind the increased lipid synthesis, we investigated the lipid binding properties of MGS <i>in vivo</i> together with the correlation between lipid synthesis and MGS overexpression levels. A good correlation between produced lipid quantities and overexpressed MGS protein was observed when standard LB medium was supplemented with four different lipid precursors that have significant roles in the lipid biosynthesis pathway. Interestingly, this correlation was highest concerning anionic lipid production and at the same time dependent on the selective binding of anionic lipid molecules by MGS. A selective interaction with anionic lipids was also observed <i>in vitro</i> by ³¹P NMR binding studies using bicelles prepared with <i>E. coli</i> lipids. The results clearly demonstrate that the discriminative withdrawal of anionic lipids, especially phosphatidylglycerol, from the membrane through MGS binding triggers an <i>in vivo</i> signal for cells to create a “feed-forward” stimulation of lipid synthesis in <i>E. coli</i>. By this mechanism, cells can produce more membrane surface in order to accommodate excessively produced MGS molecules, which results in an interdependent cycle of lipid and MGS protein synthesis

Additional file 1: of Biological mechanisms discriminating growth rate and adult body weight phenotypes in two Chinese indigenous chicken breeds

The microarray hybridization data for the breast muscle tissue. The file shows the breast muscle tissue microarray data used for the functional analyses. (XLS 10476 kb

Comparison of insulin-like growth factor-1 (IGF-1) mRNA expression.

<p>IGF-1 mRNA abundance in the liver (a), breast muscles (b) and in thigh muscles (c) from the Daweishan mini chicken and the Wuding chicken breeds, and the commercial Avian broiler hybrid at weeks 0, 4, 8 and 12. Statistical significance of difference among chickens at a specific age is indicated by asterisks (*: P<0.05; **: P<0.01). Statistical significance among different ages within a breed / hybrid is indicated with letters (lower case: P<0.05; capitals: P<0.01).</p

Comparison of insulin-like growth factor-1 binding protein 2 (IGFBP2) mRNA Expression.

<p>IGFBP mRNA abundance in the liver (a), breast muscles (b) and in thigh muscles (c) from Daweishan mini chicken and the Wuding chicken breeds, and the commercial Avian broiler hybrid at week 0, 4, 8 and 12. Statistical significance of difference among chickens at a specific age is indicated by asterisks (*: P<0.05; **: P<0.01). Statistical significance among different ages within a breed / hybrid is indicated with letters (lower case: P<0.05; capitals: P<0.01).</p

Comparison of growth hormone binding protein (GHBP) mRNA expression.

<p>GHBP mRNA abundance in the liver (a), breast muscles (b) and in thigh muscles (c) from the Daweishan mini chicken and the Wuding chicken breeds, and the commercial Avian broiler hybrid at weeks 0, 4, 8 and 12. Statistical significance of difference among chickens at a specific age is indicated by asterisks (*: P<0.05; **: P<0.01). Statistical significance among different ages within a breed / hybrid is indicated with letters (lower case: P<0.05; capitals: P<0.01).</p

Comparison of insulin-like growth factor-1 receptor (IGF-1R) mRNA expression.

<p>IGF1R mRNA abundance in the liver (a), breast muscles (b) and in thigh muscles (c) from the Daweishan mini chicken and the Wuding chicken breeds, and the commercial Avian broiler hybrid at week 0, 4, 8 and 12. Statistical significance of difference among chickens at a specific age is indicated by asterisks (*: P<0.05; **: P<0.01). Statistical significance among different ages within a breed / hybrid is indicated with letters (lower case: P<0.05; capitals: P<0.01).</p

Comparison of growth hormone (GH) mRNA expression.

<p>GH mRNA abundance in the pituitary (a), the liver (b), breast muscles (c) and in thigh muscles (d) from the Daweishan mini chicken and the Wuding chicken breeds, and the commercial Avian broiler hybrid at weeks 0, 4, 8 and 12. Statistical significance of difference among chickens at a specific age is indicated by asterisks (*: P<0.05; **: P<0.01). Statistical significance among different ages within a breed / hybrid is indicated with letters (lower case: P<0.05; capitals: P<0.01).</p