Grass-livestock interaction: a critical review of current research progress

IntroductionGrass-livestock interaction is of great value in maintaining ecological balance, regional economic development, and the sustainability of grassland husbandry, which has gained attention in recent years in both academia and practice, however, study on grass-livestock interaction possesses cognitive bias and gaps.MethodsThis study clarified the latest research progress and development trend in the grass-livestock interaction field by illuminating publication trend, cooperative network, keyword co-occurrence relationship, keyword clustering during 2000 to 2022 based on the Web of Science core database.ResultsThe results indicated that the annual number of publications in the grass-livestock interaction field showed a globally increasing with interdisciplinary and international collaboration, and the United States of America ranked firstly, followed by Brazil, England, Australia, China. Agricultural Research Service, the U.S. Department of Agricultures was the organization with the highest number of publications, and Rangeland Ecology & Management, Agriculture, Ecosystems & Environment, Grass and Forage Science were the top three journals. Furthermore, in terms of the temporal evolution, the research targeting the grass-livestock interaction mainly went through three stages: initial exploration period (2000–2011), to the period of rapid development (2012–2016) to transitional development period (2017–2022), and the notable existing keywords was Management, Livestock, Cattle, Vegetation, Growth, Soil. In terms of the structural composition, four broad divisions including mechanism of human activities, grassland resource monitoring and management, grassland livestock competition/adaptability, grassland land/soil research were identified. The results provide positive and objective insights into that grassland sustainability and grazing control through strengthening cross-disciplinary and cross-regional cooperation, and applying of standard methods based on evaluation system in the grass-livestock interaction field.DiscussionThe overall contribution of the work is provision of novel insight into the intellectual structure and temporal progression of grass-livestock interaction research at a macroscopic scale and the directions in which the field is headed currently

Patients With Obsessive-Compulsive Disorder Exhibit Deficits in Consummatory but Not Anticipatory Pleasure

Background: Reward dysfunctions have been reported in obsessive-compulsive disorder (OCD), which implicates a high possibility of anhedonia for this disease. However, several components of anhedonia, such as consummatory and anticipatory pleasure, has not been substantially studied in OCD patients.Methods: The Chinese version of the Temporal Experience of Pleasure Scale (CV-TEPS) was used to evaluate both the consummatory and anticipatory pleasure in 130 OCD patients, 89 major depressive disorder (MDD) patients, and 95 healthy controls (HCs). The Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) and the Beck Depression Inventory (BDI) were scored for assessing the severity of obsessive and compulsive symptoms and depressive symptoms, respectively. Analyses of covariance (ANCOVA) were used to compare the differences of anhedonia among the three groups with the severity of depression controlled. Regression analyses were also used to analyze the relationship between consummatory and anticipatory pleasure and clinical variables in OCD patients.Results: After controlling for the effect of depression, there were significant differences in TEPS scores among the three groups (p < 0.05). Compared with HCs, OCD patients had lower scores on the consummatory subscale, but not the anticipatory subscale, of the TEPS. MDD patients had lower scores on both the consummatory and anticipatory subscales than HCs.Conclusion: OCD patients exhibit deficits in consummatory but not anticipatory pleasure, which is distinct from MDD patients

Molecular cloning and preliminary function study of iron responsive element binding protein 1 gene from cypermethrin-resistant Culex pipiens pallens

<p>Abstract</p> <p>Background</p> <p>Insecticide resistance jeopardizes the control of mosquito populations and mosquito-borne disease control, which creates a major public health concern. Two-dimensional electrophoresis identified one protein segment with high sequence homology to part of <it>Aedes aegypti </it>iron-responsive element binding protein (IRE-BP).</p> <p>Method</p> <p>RT-PCR and RACE (rapid amplification of cDNA end) were used to clone a cDNA encoding full length IRE-BP 1. Real-time quantitative RT-PCR was used to evaluate the transcriptional level changes in the Cr-IRE strain <it>Aedes aegypti </it>compared to the susceptible strain of <it>Cx. pipiens pallens</it>. The expression profile of the gene was established in the mosquito life cycle. Methyl tritiated thymidine (³H-TdR) was used to observe the cypermethrin resistance changes in C6/36 cells containing the stably transfected IRE-BP 1 gene of <it>Cx. pipiens pallens</it>.</p> <p>Results</p> <p>The complete sequence of iron responsive element binding protein 1 (IRE-BP 1) has been cloned from the cypermethrin-resistant strain of <it>Culex pipiens pallens </it>(Cr-IRE strain). Quantitative RT-PCR analysis indicated that the IRE-BP 1 transcription level was 6.7 times higher in the Cr-IRE strain than in the susceptible strain of 4th instar larvae. The IRE-BP 1 expression was also found to be consistently higher throughout the life cycle of the Cr-IRE strain. A protein of predicted size 109.4 kDa has been detected by Western blotting in IRE-BP 1-transfected mosquito C6/36 cells. These IRE-BP 1-transfected cells also showed enhanced cypermethrin resistance compared to null-transfected or plasmid vector-transfected cells as determined by ³H-TdR incorporation.</p> <p>Conclusion</p> <p>IRE-BP 1 is expressed at higher levels in the Cr-IRE strain, and may confer some insecticide resistance in <it>Cx. pipiens pallens</it>.</p

Molecular Ecology of Pyrethroid Knockdown Resistance in Culex pipiens pallens Mosquitoes

Pyrethroid insecticides have been extensively used in China and worldwide for public health pest control. Accurate resistance monitoring is essential to guide the rational use of insecticides and resistance management. Here we examined the nucleotide diversity of the para-sodium channel gene, which confers knockdown resistance (kdr) in Culex pipiens pallens mosquitoes in China. The sequence analysis of the para-sodium channel gene identified L1014F and L1014S mutations. We developed and validated allele-specific PCR and the real-time TaqMan methods for resistance diagnosis. The real-time TaqMan method is more superior to the allele-specific PCR method as evidenced by higher amplification rate and better sensitivity and specificity. Significant positive correlation between kdr allele frequency and bioassay-based resistance phenotype demonstrates that the frequency of L1014F and L1014S mutations in the kdr gene can be used as a molecular marker for deltamethrin resistance monitoring in natural Cx. pipiens pallens populations in the East China region. The laboratory selection experiment found that L1014F mutation frequency, but not L1014S mutation, responded to deltamethrin selection, suggesting that the L1014F mutation is the key mutation conferring resistance to deltamethrin. High L1014F mutation frequency detected in six populations of Cx. pipens pallens suggests high prevalence of pyrethroid resistance in Eastern China, calling for further surveys to map the resistance in China and for investigating alternative mosquito control strategies

Naturally Occurring Incompatibilities between Different <em>Culex pipiens pallens</em> Populations as the Basis of Potential Mosquito Control Measures

<div><h3>Background</h3><p>Vector-borne diseases remain a threat to public health, especially in tropical countries. The incompatible insect technique has been explored as a potential control strategy for several important insect vectors. However, this strategy has not been tested in <em>Culex pipiens pallens</em>, the most prevalent mosquito species in China. Previous works used introgression to generate new strains that matched the genetic backgrounds of target populations while harboring a new <em>Wolbachia</em> endosymbiont, resulting in mating competitiveness and cytoplasmic incompatibility. The generation of these incompatible insects is often time-consuming, and the long-term stability of the newly created insect-<em>Wolbachia</em> symbiosis is uncertain. Considering the wide distribution of <em>Cx. pipiens pallens</em> and hence possible isolation of different populations, we sought to test for incompatibilities between natural populations and the possibility of exploiting these incompatibilities as a control strategy.</p> <h3>Methodology/Principal Findings</h3><p>Three field populations were collected from three geographic locations in eastern China. Reciprocal cross results showed that bi-directional patterns of incompatibility existed between some populations. Mating competition experiments indicated that incompatible males could compete with cognate males in mating with females, leading to reduced overall fecundity. F1 offspring from incompatible crosses maintained their maternal crossing types. All three populations tested positive for <em>Wolbachia</em>. Removal of <em>Wolbachia</em> by tetracycline rendered matings between these populations fully compatible.</p> <h3>Conclusions/Significance</h3><p>Our findings indicate that naturally occurring patterns of cytoplasmic incompatibility between <em>Cx. pipiens pallens</em> populations can be the basis of a control strategy for this important vector species. The observed incompatibilities are caused by <em>Wolbachia</em>. More tests including field trials are warranted to evaluate the feasibility of this strategy as a supplement to other control measures.</p> </div

PageRank talent mining algorithm of power system based on cognitive load and DPCNN

Abstract PageRank talent mining in power system is an effective means for enterprises to recruit talents, which can correctly recommend talents in practical applications. At present, the mining evaluation index system is not perfect, and the consistency coefficient between the evaluation results and the actual situation is low in practical applications. Therefore, PageRank talent mining algorithm in power system based on cognitive load and dilated convolutional neural network (DPCNN) is proposed. The cognitive load and DPCNN are used to establish a talent capability evaluation system, calculate the index weight value, construct the PageRank talent capability evaluation model of the power system according to the corresponding weight of the index, determine the membership range of the index, calculate the comprehensive score of the appraiser's ability, and determine the ability level of the appraiser, thus realizing the PageRank talent mining algorithm of the power system. The experimental results show that the algorithm has high accuracy and objectivity, good encryption effect, cannot crack the attack node, the prediction error and the prediction relative error are closest to the standard value, the maximum error is 0.51, the maximum relative error is 0.82, and can achieve the accurate prediction of talent demand

Impacts of 3DEnVar-Based FY-3D MWHS-2 Radiance Assimilation on Numerical Simulations of Landfalling Typhoon Ampil (2018)

The module for assimilating radiance data of the Microwave Humidity Sounder-2 (MWHS-2) onboard the Feng Yun 3D (FY-3D) satellite is built in the Weather Research and Forecasting (WRF) model data assimilation (WRFDA) system. The CONV, 3DVar, and EnVar experiments are conducted to investigate the impact of assimilating the new humidity sounder based on Typhoon Ampil (2018). Both the 3DVar and EnVar experiments assimilate FY-3D MWHS-2 radiance data on top of the conventional data, while the CONV experiment only applies conventional data. In the EnVar experiment, notable geopotential height increment is observed around the typhoon, leading the typhoon to move northeast. In addition, the moisture field is improved to some extent. Finally, from the analysis of the dynamic field of the typhoon, it can be found that the EnVar experiment can adjust the dynamic structure of the typhoon. Furthermore, the assimilation of FY-3D MWHS-2 radiance data reduces the forecast error of the typhoon track and intensity. Additionally, the precipitation skill is improved in terms of rainfall pattern and the verification score. This improvement in the precipitation may be closely related to the features of the circulation structure concerning the evolution of the typhoon. The improved prediction of the position and intensity of rainbands in the FY-3D MWHS-2 radiance data assimilation experiment corresponds to a better prediction of typhoon structure