Successful transplantation of guinea pig gut microbiota in mice and its effect on pneumonic plague sensitivity

Microbiota-driven variations in the inflammatory response are predicted to regulate host responses to infection. Increasing evidence indicates that the gastrointestinal and respiratory tracts have an intimate relationship with each other. Gut microbiota can influence lung immunity whereby gut-derived injurious factors can reach the lungs and systemic circulation via the intestinal lymphatics. The intestinal microbiota’s ability to resist colonization can be extended to systemic infections or to pathogens infecting distant sites such as the lungs. Unlike the situation with large mammals, the microtus Yersinia pestis 201 strain exhibits strong virulence in mice, but nearly no virulence to large mammals (such as guinea pigs). Hence, to assess whether the intestinal microbiota from guinea pigs was able to affect the sensitivity of mice to challenge infection with the Y. pestis 201 strain, we fed mice with guinea pig diets for two months, after which they were administered 0.5 ml of guinea pig fecal suspension for 30 days by oral gavage. The stools from each mouse were collected on days 0, 15, and 30, DNA was extracted from them, and 16S rRNA sequencing was performed to assess the diversity and composition of the gut microbiota. We found that the intestinal microbiota transplants from the guinea pigs were able to colonize the mouse intestines. The mice were then infected with Yersinia pestis 201 by lung invasion, but no statistical difference was found in the survival rates of the mice that were colonized with the guinea pig’s gut microbiota and the control mice. This indicates that the intestinal microbiota transplantation from the guinea pigs did not affect the sensitivity of the mice to pneumonic plague

Fermion-boson many-body interplay in a frustrated kagome paramagnet

Kagome-net, appearing in areas of fundamental physics, materials, photonic and cold-atom systems, hosts frustrated fermionic and bosonic excitations. However, it is extremely rare to find a system to study both fermionic and bosonic modes to gain insights into their many-body interplay. Here we use state-of-the-art scanning tunneling microscopy and spectroscopy to discover unusual electronic coupling to flat-band phonons in a layered kagome paramagnet. Our results reveal the kagome structure with unprecedented atomic resolution and observe the striking bosonic mode interacting with dispersive kagome electrons near the Fermi surface. At this mode energy, the fermionic quasi-particle dispersion exhibits a pronounced renormalization, signaling a giant coupling to bosons. Through a combination of self-energy analysis, first-principles calculation, and a lattice vibration model, we present evidence that this mode arises from the geometrically frustrated phonon flat-band, which is the lattice analog of kagome electron flat-band. Our findings provide the first example of kagome bosonic mode (flat-band phonon) in electronic excitations and its strong interaction with fermionic degrees of freedom in kagome-net materials.Comment: To appear in Nature Communications (2020

Observation of sixfold degenerate fermions in PdSb2_2

Three types of fermions have been extensively studied in topological quantum materials: Dirac, Weyl, and Majorana fermions. Beyond the fundamental fermions in high energy physics, exotic fermions are allowed in condensed matter systems residing in three-, six- or eightfold degenerate band crossings. Here, we use angle-resolved photoemission spectroscopy to directly visualize three-doubly-degenerate bands in PdSb$_2$. The ultrahigh energy resolution we are able to achieve allows for the confirmation of all the sixfold degenerate bands at the R point, in remarkable consistency with first-principles calculations. Moreover, we find that this sixfold degenerate crossing has quadratic dispersion as predicted by theory. Finally, we compare sixfold degenerate fermions with previously confirmed fermions to demonstrate the importance of this work: our study indicates a topological fermion beyond the constraints of high energy physics

The genome of broomcorn millet

Broomcorn millet (Panicum miliaceum L.) is the most water-efficient cereal and one of the earliest domesticated plants. Here we report its high-quality, chromosome-scale genome assembly using a combination of short-read sequencing, single-molecule real-time sequencing, Hi-C, and a high-density genetic map. Phylogenetic analyses reveal two sets of homologous chromosomes that may have merged ~5.6 million years ago, both of which exhibit strong synteny with other grass species. Broomcorn millet contains 55,930 proteincoding genes and 339 microRNA genes. We find Paniceae-specific expansion in several subfamilies of the BTB (broad complex/tramtrack/bric-a-brac) subunit of ubiquitin E3 ligases, suggesting enhanced regulation of protein dynamics may have contributed to the evolution of broomcorn millet. In addition, we identify the coexistence of all three C4 subtypes of carbon fixation candidate genes. The genome sequence is a valuable resource for breeders and will provide the foundation for studying the exceptional stress tolerance as well as C4 biology

Fault diagnosis and energy consumption analysis for variable air volume air conditioning system: a case study

Several common faults and their causes in variable air volume (VAV) air conditioning system are presented, and the principle of fault diagnosis of air conditioning system is briefly described. The VAV air conditioning system was modeled in TRNSYS, and five typical faults of the cooling mode were simulated. The comparative analysis of the respective under normal operation and fault operation had been made, and the impact of each fault on the energy consumption was also analyzed. The actual operating characteristics of air conditioning system was then evaluated. Further, some parameters under fault operations were compared with those under the normal operation, from which the changing characteristics of parameters could be discovered, and the characteristics can be used to diagnose faults. The simulation results demonstrate that fault can affect the energy consumption of VAV air conditioning system, and the impact of each fault is different. In addition, monitoring the change of energy consumption and operation parameters is helpful in fault diagnosis, and the effective fault diagnosis has great significance to energy-saving of the air conditioning system

Experimental study of hydraulic stability for variable water volume air conditioning System

The hydraulic stability of the variable water volume air conditioning system has a significant impact on the thermodynamic stability, service life of the pipe network and energy consumption. The hydraulic stability of variable water volume air conditioning system was investigated by means of experiments. An air conditioning system with variable water volume is served as the platform, and then the changes of the passive branches’ hydraulic characteristics are studied in valve action cycle of the active branch under the following three conditions: without pump control, constant differential pressure (DP) control or variable differential pressure set-point control. The results show that the branch with the small flow and high-pressure drop has the good hydraulic stability. Under the pressure difference control, the hydraulic stability of the system is improved obviously, the energy-saving effect of the pump is better, and the energy saving of the chilled water pump at least 20.8% when closed any branch valve. This study provides a reference for the design of variable water volume air conditioning systems with good hydraulic stability

Dynamical analysis of a fractional-order nonlinear two-degree-of-freedom vehicle system by incremental harmonic balance method

At present, there are few studies considering both nonlinear and fractional characteristics of suspension in vehicle systems. In this paper, a fractional nonlinear model of a quarter vehicle with two-degree-of-freedom (2-DOF) is innovatively proposed to describe the suspension system containing the viscoelastic material metal rubber. Given the lack of a general calculation scheme for the multi-degree-of-freedom fractional-order incremental harmonic balance method (IHBM), a general calculation scheme for the 2-DOF incremental harmonic balance method for nonlinear systems with fractional order is derived. The nonlinear dynamical properties of the presented model are acquired using this method. The accuracy of the proposed method is verified through a comparison with the power series expansion method. Afterward, the effects of the various parameters on the dynamic performance are analyzed. The vibration peak value of the fractional-order model is significantly higher than that of the integer-order model (IOM). Therefore, the suspension parameters should be designed with a margin when using IOM

The complete mitochondrial genome of the Spotted Greenshank Tringa guttifer (Charadriiforemes: Charadriidae)

The complete mitochondrial DNA genome of the Endangered Spotted Greenshank, Tringa guttifer, was detected using phenol–chloroform extraction procedure and polymerase chain reaction method. It is a circular molecule of 16,935 bp in size, exhibits the typical structure of mitochondrial genomes of Charadriiformes, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. Overall base composition of the complete mitochondrial DNA is A (31.7%), T (25.5%), C (29.5%), and G (13.3%), the percentage of A and T (57.2%) is higher than G and C. The phylogenetic relationships of 34 species were reconstructed using the maximum-likelihood and Bayesian inference, and unravel T. guttifer is close to T. semipalmata