3D model of amphioxus steroid receptor complexed with estradiol

The origins of signaling by vertebrate steroids are not fully understood. An important advance was the report that an estrogen-binding steroid receptor [SR] is present in amphioxus, a basal chordate with a similar body plan as vertebrates. To investigate the evolution of estrogen binding to steroid receptors, we constructed a 3D model of amphioxus SR complexed with estradiol. This 3D model indicates that although the SR is activated by estradiol, some interactions between estradiol and human ER[alpha] are not conserved in the SR, which can explain the low affinity of estradiol for the SR. These differences between the SR and ER[alpha] in the steroid-binding domain are sufficient to suggest that another steroid is the physiological regulator of the SR. The 3D model predicts that mutation of Glu-346 to Gln will increase the affinity of testosterone for amphioxus SR and elucidate the evolution of steroid binding to nuclear receptors

Off-resonance magnetisation transfer contrast (MTC) MRI using fast field-cycling (FFC)

Peer reviewedPostprin

Design and construction of an actively frequency-switchable RF coil for field-dependent Magnetisation Transfer Contrast MRI with fast field-cycling

Copyright © 2010 Elsevier Inc. All rights reserved.Peer reviewedPostprin

Flavor SU(4) breaking between effective couplings

Using a framework in which all elements are constrained by Dyson-Schwinger equation studies in QCD, and therefore incorporates a consistent, direct and simultaneous description of light- and heavy-quarks and the states they constitute, we analyze the accuracy of SU(4)-flavor symmetry relations between {\pi}{\rho}{\pi}, K{\rho}K and D{\rho}D couplings. Such relations are widely used in phenomenological analyses of the interactions between matter and charmed mesons. We find that whilst SU(3)-flavor symmetry is accurate to 20%, SU(4) relations underestimate the D{\rho}D coupling by a factor of five.Comment: 5 pages, two figure

High Performance, Continuously Tunable Microwave Filters using MEMS Devices with Very Large, Controlled, Out-of-Plane Actuation

Software defined radios (SDR) in the microwave X and K bands offer the promise of low cost, programmable operation with real-time frequency agility. However, the real world in which such radios operate requires them to be able to detect nanowatt signals in the vicinity of 100 kW transmitters. This imposes the need for selective RF filters on the front end of the receiver to block the large, out of band RF signals so that the finite dynamic range of the SDR is not overwhelmed and the desired nanowatt signals can be detected and digitally processed. This is currently typically done with a number of narrow band filters that are switched in and out under program control. What is needed is a small, fast, wide tuning range, high Q, low loss filter that can continuously tune over large regions of the microwave spectrum. In this paper we show how extreme throw MEMS actuators can be used to build such filters operating up to 15 GHz and beyond. The key enabling attribute of our MEMS actuators is that they have large, controllable, out-of-plane actuation ranges of a millimeter or more. In a capacitance-post loaded cavity filter geometry, this gives sufficient precisely controllable motion to produce widely tunable devices in the 4-15 GHz regime.Comment: 12 pages 14 figures 2 table

Nox2 redox signaling maintains essential cell populations in the brain.

Reactive oxygen species (ROS) are conventionally classified as toxic consequences of aerobic life, and the brain is particularly susceptible to ROS-induced oxidative stress and damage owing to its high energy and oxygen demands. NADPH oxidases (Nox) are a widespread source of brain ROS implicated in seizures, stroke and neurodegeneration. A physiological role for ROS generation in normal brain function has not been established, despite the fact that mice and humans lacking functional Nox proteins have cognitive deficits. Using molecular imaging with Peroxyfluor-6 (PF6), a new selective fluorescent indicator for hydrogen peroxide (H(2)O(2)), we show that adult hippocampal stem/progenitor cells (AHPs) generate H(2)O(2) through Nox2 to regulate intracellular growth signaling pathways, which in turn maintains their normal proliferation in vitro and in vivo. Our results challenge the traditional view that brain ROS are solely deleterious by demonstrating that controlled ROS chemistry is needed for maintaining specific cell populations

Features and flaws of a contact interaction treatment of the kaon

Elastic and semileptonic transition form factors for the kaon and pion are calculated using the leading-order in a global-symmetry-preserving truncation of the Dyson-Schwinger equations and a momentum-independent form for the associated kernels in the gap and Bethe-Salpeter equations. The computed form factors are compared both with those obtained using the same truncation but an interaction that preserves the one-loop renormalisation-group behaviour of QCD and with data. The comparisons show that: in connection with observables revealed by probes with |Q^2|<~ M^2, where M~0.4GeV is an infrared value of the dressed-quark mass, results obtained using a symmetry-preserving regularisation of the contact-interaction are not realistically distinguishable from those produced by more sophisticated kernels; and available data on kaon form factors do not extend into the domain whereupon one could distinguish between the interactions. The situation is different if one includes the domain Q^2>M^2. Thereupon, a fully consistent treatment of the contact interaction produces form factors that are typically harder than those obtained with QCD renormalisation-group-improved kernels. Amongst other things also described are a Ward identity for the inhomogeneous scalar vertex, similarity between the charge distribution of a dressed-u-quark in the K^+ and that of the dressed-u-quark in the pi^+, and reflections upon the point whereat one might begin to see perturbative behaviour in the pion form factor. Interpolations of the form factors are provided, which should assist in working to chart the interaction between light-quarks by explicating the impact on hadron properties of differing assumptions about the behaviour of the Bethe-Salpeter kernel.Comment: 17 pages, 9 figures, 4 table

Mems device with large out-of-plane actuation and low-resistance interconnect and methods of use

Source: United States Patent and Trademark Office, www.uspto.gov”The present application is directed to a MEMS device. The MEMS device includes a substrate having a first end and a second end extending along a longitudinal axis, the Substrate including an electrostatic actuator. The device also includes a movable plate having a first end and a second end. The device also includes a thermal actuator having a first end coupled to the first end of the substrate and a second end coupled to the first end of the plate. The actuator moves the plate in relation to the substrate. Further, the device includes a power source electrically coupled to the thermal actuator and the Substrate. The application is also directed to a method for operating a MEMS device