Additional file 1: of The fortune cookie flap for aesthetic reconstruction after chest keloid resection: a small case series

Previous treatment history at other hospital. (A) A 31-year-old man with a keloid on the anterior chest wall. (B) The patient had a 7.0 × 3.0 cm defect after resection of a keloid. (C) He underwent wound closure using a traditional subcuticular purse-string suture at the other clinic. (D) 5 weeks after surgery, the wound was dehisced and methicillin resistant Staphylococcus aureus (MRSA) was identified in the wound. (JPEG 84 kb

Ultimate Control of Rate-Dependent Adhesion for Reversible Transfer Process via a Thin Elastomeric Layer

Adhesion between a stamp with an elastomeric layer and various devices or substrates is crucial to successfully fabricate flexible electronics using a transfer process. Although various transfer processes using stamps with different adhesion strengths have been suggested, the controllable range of adhesion is still limited to a narrow range. To precisely transfer devices onto selected substrates, however, the difference in adhesion between the picking and placing processes should be large enough to achieve a high yield. Herein, we report a simple way to extend the controllable adhesion range of stamps, which can be achieved by adjusting the thickness of the elastomeric layer and the separation velocity. The adhesion strength increased with decreasing layer thickness on the stamp due to a magnification of the confinement and rate-dependent effects on the adhesion. This enabled the controllable range of the adhesion strength for a 15 μm-thick elastomeric layer to be extended up to 12 times that of the bulk under the same separation conditions. The strategy of designing stamps using simple adhesion tests is also introduced, and the reversible transfer of thin Si chips was successfully demonstrated. Tuning and optimizing the adhesion strength of a stamp according to the design process suggested here can be applied to various materials for the selective transfer and replacement of individual devices

Ultimate Control of Rate-Dependent Adhesion for Reversible Transfer Process via a Thin Elastomeric Layer

Adhesion between a stamp with an elastomeric layer and various devices or substrates is crucial to successfully fabricate flexible electronics using a transfer process. Although various transfer processes using stamps with different adhesion strengths have been suggested, the controllable range of adhesion is still limited to a narrow range. To precisely transfer devices onto selected substrates, however, the difference in adhesion between the picking and placing processes should be large enough to achieve a high yield. Herein, we report a simple way to extend the controllable adhesion range of stamps, which can be achieved by adjusting the thickness of the elastomeric layer and the separation velocity. The adhesion strength increased with decreasing layer thickness on the stamp due to a magnification of the confinement and rate-dependent effects on the adhesion. This enabled the controllable range of the adhesion strength for a 15 μm-thick elastomeric layer to be extended up to 12 times that of the bulk under the same separation conditions. The strategy of designing stamps using simple adhesion tests is also introduced, and the reversible transfer of thin Si chips was successfully demonstrated. Tuning and optimizing the adhesion strength of a stamp according to the design process suggested here can be applied to various materials for the selective transfer and replacement of individual devices

Additional file 1: Table S1. of Bone alkaline phosphatase as a surrogate marker of bone metastasis in gastric cancer patients

Sites of bone metastasis. (DOCX 14 kb

Additional file 1: Table S1. of Role of adjuvant chemotherapy in locally advanced rectal cancer with ypT0-3N0 after preoperative chemoradiation therapy and surgery

Effect of adjuvant chemotherapy on disease-free survival and overall survival by patient demographics and tumor characteristics in the entire sample of patients. Table S2. Effect of adjuvant chemotherapy on disease-free survival and overall survival by patient demographics and tumor characteristics in the cohort of propensity score-matched patients. Table S3. Recurrence or death events at different times after surgery. Comparisons were done by Fisher’s exact test. Table S4. Effect of adjuvant chemotherapy on disease-free survival and overall survival by restricting analysis to patients who remained event-free at different times after surgery. (DOCX 30 kb

Additional file 1: Figure S1. of Prognostic implications of PD-L1 expression in patients with soft tissue sarcoma

Survival analyses according to PD-L1 expression in patients with localized disease. (A) Kaplan-Meier survival curves for recurrence-free survival (RFS). (B) Kaplan-Meier survival curves for overall survival (OS). (TIF 84 kb

No significant differences of Th2 cells in the SI LP and serum total IgE, UDE-specific IgE and IgG1 levels between UDE- and PBS-administered mice.

<div><p>(A) Frequency and number of IL-4/GFP⁺ CD4⁺ T cells in the SI LP of UDE-versus PBS-administered control 4get mice.</p> <p>(B) Serum total IgE, UDE-specific IgE, and IgG1 secretion analyzed by ELISA.</p> <p>NS= not significant, OD₄₅₀=optical density at absorbance 450nm.</p></div

Significant increase of IL-4-expressing eosinophils in the SI LP of UDE-administered mice.

<div><p>(A) Cells isolated from SI LP were stained with CD11b, CD11c, and MHC class II and analyzed by flow cytometry. MHC class II ^lowCD11b^high CD11c^int cells (R1) are eosinophils. Among MHC class II^high cells, CD11b ^intCD11c^int cells (R4) and CD11c^high cells (R2 and R3) correspond to macrophages and dendritic cells, respectively.</p> <p>(B) Proportion and number of eosinophils, macrophages, and dendritic cells in the SP of UDE- versus PBS-administered control mice.</p> <p>(C) Antigen presenting cells isolated from SI LP were stained with CD40, CD80, and CD86 and analyzed by flow cytometry. Empty, solid line histograms represent cells from UDE-administered (red) versus PBS-administered control mice (black). Shaded histograms represent cells stained with isotype control of UDE-administered (dark gray) versus PBS-administered control mice (gray).</p> <p>(D) Mean fluorescence intensity of IL-4/GFP expressed by APCs from the SI LP of 4get mice were analyzed by flow cytometry.</p> <p>(E) Analysis of CD11b^highCD11c^int cells from LP of WT and ΔdblGATA mice for eosinophil markers CCR3 and Siglec F.</p> <p>*p<0.05, **p < 0.01, NS= not significant, LP: lamina propria, EO: eosionophil, DC: dendritic cell, Mϕ: macrophage, MFI: mean fluorescence intensity.</p></div

Decreased number of CD4⁺ T cells in the SI LP of UDE-administered mice.

<div><p>(A) Cells isolated from spleen (SP), mesenteric lymph node (MLN), and small intestinal lamina propria (SI LP) were stained with TCRβ and CD19 and analyzed by flow cytometry.</p> <p>(B) Frequency of T and B cells in the SP, MLN, and SI LP of UDE- versus PBS-administered control mice.</p> <p>(C) T Cells from SP, MLN, and SI LP were stained with CD4 and CD8α and analyzed by flow cytometry.</p> <p>(D) Frequency of CD4 and CD8 cells in the SP, MLN, and SI LP of UDE- versus PBS-administered control mice.</p> <p>(E) Total number of T/B cells and CD4/CD8 cells in the SP, MLN, and SI LP of UDE- versus PBS administered control mice.</p> <p>*p < 0.05, **p < 0.01, NS=not significant.</p></div