Exploring the developmental changes in automatic two-digit number processing

Even when two-digit numbers are irrelevant to the task at hand, adults process them. Do children process numbers automatically, and if so, what kind of information is activated? In a novel dot-number Stroop task, children (Grades 1-5) and adults were shown two different two-digit numbers made up of dots. Participants were asked to select the number that contained the larger dots. If numbers are processed automatically, reaction time for dot size judgment should be affected by numerical characteristics. The results suggest that, like adults, children process two-digit numbers automatically. Based on the current findings, we propose a developmental trend for automatic two-digit number processing that goes from decomposed sequential (activation of decade digit followed by that of unit digit) to decomposed parallel processing (simultaneous activation of decade and unit digits). © 2011 Elsevier Inc.postprin

Can non-interactive language input benefit young second-language learners?

To fully acquire a language, especially its phonology, children need linguistic input from native speakers early on. When interaction with native speakers is not always possible – e.g. for children learning a second language that is not the societal language – audios are commonly used as an affordable substitute. But does such non-interactive input work? Two experiments evaluated the usefulness of audio storybooks in acquiring a more native-like second-language accent. Young children, first- and second-graders in Hong Kong whose native language was Cantonese Chinese, were given take-home listening assignments in a second language, either English or Putonghua Chinese. Accent ratings of the children's story reading revealed measurable benefits of non-interactive input from native speakers. The benefits were far more robust for Putonghua than English. Implications for second-language accent acquisition are discussed.postprin

Formation of virus-like particles from human cell lines exclusively expressing Influenza Neuraminidase

The minimal virus requirements for the generation of influenza virus-like particle (VLP) assembly and budding were reassessed. Using neuraminidase (NA) from the H5N1 and H1N1 subtypes, it was found that the expression of NA alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA-containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymic activity, whilst not required for the release of NA-VLPs, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis, and demonstrate that NA-VLPs represent a useful tool in influenza research. © 2010 SGM.link_to_OA_fulltex

Comparison of post-treatment plasma EBV DNA with nasopharyngeal biopsy in patients after radical (chemo) radiotherapy for non-metatatic nasopharyngeal cancer

This journal suppl. entitled: Proceedings of the American Society for Radiation Oncology 56th Annual Meeting, ASTRO's 56th Annual Meeting ... 2014Oral Scientific SessionPURPOSE/OBJECTIVE(S): Random nasopharyngeal biopsy after completion of intensity-modulated radiation therapy (IMRT) for non-metastatic nasopharyngeal cancer (NPC) is routinely practiced in Hong Kong to confirm local remission. Plasma EBV DNA is proven an accurate marker for NPC. We carried out a prospective study comparing the correlation between post-IMRT nasopharyngeal biopsy and EBV DNA, to investigate if EBV DNA can substitute biopsy to confirm local remission. MATERIALS/METHODS: Patients with non-metastatic NPC treated with definitive (chemo) IMRT diagnosed between January 2011 and March 2013 were recruited. After baseline workup ...postprin

Inhibition of HIV virus by neutralizing Vhh attached to dual functional liposomes encapsulating dapivirine

Although highly active antiretroviral therapy (HAART) has greatly improved the life expectancy of HIV/AIDS patients, the treatment is not curative. It is a global challenge which fosters an urgent need to develop an effective drug or neutralizing antibody delivery approach for the prevention and treatment of this disease. Due to the low density of envelope spikes with restricted mobility present on the surface of HIV virus, which limit the antibody potency and allow virus mutation and escape from the immune system, it is important for a neutralizing antibody to form bivalent or multivalent bonds with the virus. Liposome constructs could fulfil this need due to the flexible mobility of the membrane with its attached antibodies and the capacity for drug encapsulation. In this study, we evaluated the neutralization activity of a range of liposome formulations in different sizes coated with anti-gp120 llama antibody fragments (Vhhs) conjugated via either non-covalent metal chelation or a covalent linkage. The non-covalent construct demonstrated identical binding affinity to HIV-1 envelope glycoprotein gp120 and neutralizing ability for HIV virus as free Vhh. Although covalently linked Vhh showed significant binding affinity to gp120, it unexpectedly had a lower neutralization potency. This may be due to the comparability in size of the viral and liposome particles restricting the number which can be bound to the liposome surface so involving only a fraction of the antibodies, whereas non-covalently attached antibodies dissociate from the surface after acting with gp120 and free the remainder to bind further viruses. Covalently conjugated Vhh might also trigger the cellular uptake of a liposome-virion complex. To explore the possible ability of the antibody-coated liposomes to have a further function, we encapsulated the hydrophobic antiviral drug dapivirine into both of the non-covalently and covalently conjugated liposome formulations, both of which revealed high efficacy in reducing viral replication in vitro. Thus, dual function liposomes may lead to a novel strategy for the prophylaxis of HIV/AIDS by combining the neutralizing activity of Vhh with antiviral effects of high drug concentrations

Plastid phylogenomics resolves ambiguous relationships within the orchid family and provides a solid timeframe for biogeography and macroevolution

Recent phylogenomic analyses based on the maternally inherited plastid organelle have enlightened evolutionary relationships between the subfamilies of Orchidaceae and most of the tribes. However, uncertainty remains within several subtribes and genera for which phylogenetic relationships have not ever been tested in a phylogenomic context. To address these knowledge-gaps, we here provide the most extensively sampled analysis of the orchid family to date, based on 78 plastid coding genes representing 264 species, 117 genera, 18 tribes and 28 subtribes. Divergence times are also provided as inferred from strict and relaxed molecular clocks and birth–death tree models. Our taxon sampling includes 51 newly sequenced plastid genomes produced by a genome skimming approach. We focus our sampling efforts on previously unplaced clades within tribes Cymbidieae and Epidendreae. Our results confirmed phylogenetic relationships in Orchidaceae as recovered in previous studies, most of which were recovered with maximum support (209 of the 262 tree branches). We provide for the first time a clear phylogenetic placement for Codonorchideae within subfamily Orchidoideae, and Podochilieae and Collabieae within subfamily Epidendroideae. We also identify relationships that have been persistently problematic across multiple studies, regardless of the different details of sampling and genomic datasets used for phylogenetic reconstructions. Our study provides an expanded, robust temporal phylogenomic framework of the Orchidaceae that paves the way for biogeographical and macroevolutionary studies.Universidad de Costa Rica/[814-B8-257]/UCR/Costa RicaUniversidad de Costa Rica/[814-B6-140]/UCR/Costa RicaIDEA WILD/[]//Estados UnidosSociedad Colombiana de Orquideología/[]/SCO/ColombiaFundação de Amparo à Pesquisa do Estado de São Paulo/[11/08308-9]/FAPESP/BrasilFundação de Amparo à Pesquisa do Estado de São Paulo/[13/19124-1]/FAPESP/BrasilSwiss Orchid Foundation/[]//SuizaRoyal Botanic Gardens, Kew/[]//InglaterraSwedish Research Council/[2019-05191]//SueciaSwedish Foundation for Strategic Research/[FFL15-0196]/SSF/SueciaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Jardín Botánico Lankester (JBL

How Do They Do It? – Understanding the Success of Marine Invasive Species

From the depths of the oceans to the shallow estuaries and wetlands of our coasts, organisms of the marine environment are teeming with unique adaptations to cope with a multitude of varying environmental conditions. With millions of years and a vast volume of water to call their home, they have become quite adept at developing specialized and unique techniques for survival and – given increasing human mediated transport – biological invasions. A growing world human population and a global economy drives the transportation of goods across the oceans and with them invasive species via ballast water and attached to ship hulls. In any given 24-hour period, there are about 10,000 species being transported across different biogeographic regions. If any of them manage to take hold and establish a range in an exotic habitat, the implications for local ecosystems can be costly. This review on marine invasions highlights trends among successful non-indigenous species (NIS), from vectors of transport to ecological and physiological plasticity. Apart from summarizing patterns of successful invasions, it discusses the implications of how successfully established NIS impact the local environment, economy and human health. Finally, it looks to the future and discusses what questions need to be addressed and what models can tell us about what the outlook on future marine invasions is

Understanding and processing numbers among Chinese children.

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The origin of spatial-number association

Poster Session 1: no. 1-021Numbers are associated with space. Is such association due to the quantity or ordinal aspect of numbers? Kindergarteners exhibited spatial-numerical association with ordinal numbers but not with non-numerical quantities, pointing to the ordinal aspect of numbers as the origin of spatial-numerical association

Using target-distractor discriminability to examine specific task strategies in repetition blindness

Vision: the Journal of the Vision Society of Japan, v.22 suppl. is proceedings of Asia-Pacific Conference on Vision (2010)Repetition Blindness (RB) refers to a reduced performance in reporting repeated items in a rapid serial visual presentation (RSVP) stream. Whether the size of RB is influenced by using targets and distractors of different categories (e.g., digits versus letters) has been a controversial issue in recent years. In this study, we investigated the effect of categorical discriminability on RB, by asking participants to either report the targets or to detect repetition. In Experiment 1, targets were letters and distractors were digits. Significant RB was found in the detection task, but priming was found in the report task. In Experiment 2, distractors were collections of letter segments, which rendered targets less discriminable from the distractors. RB was still found in the detection task, but unlike Experiment 1 no priming was found in the report task. Comparing the two experiments, categorical discriminability had an effect on performance in the report task, but not in the detection task. This result suggests that different strategies may be involved across tasks, so that target-distractor discriminability influences RB differently in the two tasks.The 6th Asia-Pacific Conference on Vision (APCV 2010), Taipei, Taiwan, 23-26 July 2010. In Vision, 2010, v. 22 suppl., p. 78, abstract no. 33.2