8 research outputs found
Solubilization of Proteins in 2DE: An Outline
Get PDFProtein solubilization for two-dimensional electrophoresis (2DE) has to break
molecular interactions to separate the biological contents of the material of
interest into isolated and intact polypeptides. This must be carried out in
conditions compatible with the first dimension of 2DE, namely isoelectric
focusing. In addition, the extraction process must enable easy removal of any
nonprotein component interfering with the isoelectric focusing. The constraints
brought in this process by the peculiar features of isoelectric focusing are
discussed, as well as their consequences in terms of possible solutions and
limits for the solubilization process
Metal containing proteins in the apoptosis and redox processes in the rat prostate and human prostate cells
No full textPreparative Isolation of Immune Complexes from Serum by Sucrose Gradient Ultracentrifugation
No full textMetal-Containing Proteins in the Apoptosis and Redox Processes in the Rat Prostate and Human Prostate Cells
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The histidine kinase CpHK2 has impact on spore germination, oxidative stress and fungicide resistance, and virulence of the ergot fungus Claviceps purpurea
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