Stromal CD4+ and CD8+ T Cells in Human Breast Carcinomas: Its Correlation with Chemokine MIG/CXCL9

The interaction between some chemokines with tumoral and stromal cells can influence tumor progression. CXCL9, a monokine induced by interferon gamma (MIG), targets lymphocytes. The aim of our study was to identify and quantify CD4+ and CD8+ T cells in the stroma of human breast cancer and correlate them with the presence of MIG/CXCL9. In 58 specimens of human breast carcinoma and 10 normal breast tissue from mammoplasty surgery, immunohistochemis- try and ELISA methods were performed. The number of CD4+ and CD8+ T cells in breast cancer tissue was signifi-cantly increased compared with normal breast tissue with a clear predominance of CD8+ T cells. MIG/CXCL9 levels were significantly elevated respect normal breast tissue. This chemokine correlated with the number of CD8+ T cells only in non-metastatic tumors. These data suggest that MIG targets cytotoxic T cells. Their recruitment into breast car-cinoma can play a critical role in malignant progression, inhibiting the production of metastasis.Facultad de Ciencias Médicas (FCM

Stromal CD4+ and CD8+ T Cells in Human Breast Carcinomas: Its Correlation with Chemokine MIG/CXCL9

The interaction between some chemokines with tumoral and stromal cells can influence tumor progression. CXCL9, a monokine induced by interferon gamma (MIG), targets lymphocytes. The aim of our study was to identify and quantify CD4+ and CD8+ T cells in the stroma of human breast cancer and correlate them with the presence of MIG/CXCL9. In 58 specimens of human breast carcinoma and 10 normal breast tissue from mammoplasty surgery, immunohistochemis- try and ELISA methods were performed. The number of CD4+ and CD8+ T cells in breast cancer tissue was signifi-cantly increased compared with normal breast tissue with a clear predominance of CD8+ T cells. MIG/CXCL9 levels were significantly elevated respect normal breast tissue. This chemokine correlated with the number of CD8+ T cells only in non-metastatic tumors. These data suggest that MIG targets cytotoxic T cells. Their recruitment into breast car-cinoma can play a critical role in malignant progression, inhibiting the production of metastasis.Facultad de Ciencias Médicas (FCM

Differences in resistance to reinfection with low and high inocula of <i>Trypanosoma cruzi</i> in chagasic mice treated with nifurtimox and relation to immune response

Reinfection of chronic chagasic mice after treatment with nifurtimox resulted in different outcomes according to the number of parasites used for inoculation. Nifurtimox-treated chagasic animals injected with 2,500 trypomastigotes developed higher parasitemia and increased mortality compared with nontreated chagasic mice. When reinfection was done with 25 trypomastigotes, treated and nontreated animals showed similar parasitemias and mortalities, which were significantly higher in nonchagasic controls infected for the first time. Immunological studies showed that treatment with nifurtimox led to a decrease in anti-Trypanosoma cruzi antibodies engaged in parasite destruction, inducing either complement-dependent lysis or antibodydependent cytotoxicity, but no difference in anti-T. cruzi cell-mediated immunity was found between treated and nontreated chagasic animals. It is concluded that treatment with nifurtimox leads to a loss of resistance to reinfection with a large number of trypanosomes, which is maintained with challenge with a few parasites, and that these two thresholds of premunition are probably associated with humoral and cell-mediated anti-T. cruzi immune responses, respectively.Facultad de Ciencias Médica

Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>

Sera of mice chronically infected with Trypanosoma cruzi contain antibodies that bind to the surface of living adult syngeneic heart muscle cells. In a syngeneic system, with nonadherent spleen mononuclear cells as effector cells and cardiocytes as targets, antibody-dependent cytotoxicity (ADCC), revealed by the liberation of creatine phosphokinase from damaged cardiocytes, was observed after incorporation of serum samples from infected mice. Target damage was decreased after absorption with syngeneic myocardium, but absorption with T. cruzi epimastigotes or trypomastigotes or with syngeneic skeletal muscle had no effect on ADCC. No complement-dependent lysis against heart muscle cells was detected in the same serum samples. These observations indicate that serum from chronically chagasic mice contain antibodies that bind to the surface of living adult syngeneic cardiocytes and are able to exert ADCC, suggesting that they could play a role in the pathogenesis of the heart damage that occurs in Chagas' disease.Facultad de Ciencias Médica

Presence of anti-<i>Trypanosoma cruzi</i> antibodies in the sera of mice with experimental autoimmune myocarditis

The existence of antigens shared in common by T. cruzi and heart muscle cells is suggested by the presence of antibodies binding to the parasite surface in the serum of mice with autoimmune myocarditis induced by immunization with syngeneic heart antigens.Facultad de Ciencias Médica

Stromal CD4+ and CD8+ T Cells in Human Breast Carcinomas: Its Correlation with Chemokine MIG/CXCL9

The interaction between some chemokines with tumoral and stromal cells can influence tumor progression. CXCL9, a monokine induced by interferon gamma (MIG), targets lymphocytes. The aim of our study was to identify and quantify CD4+ and CD8+ T cells in the stroma of human breast cancer and correlate them with the presence of MIG/CXCL9. In 58 specimens of human breast carcinoma and 10 normal breast tissue from mammoplasty surgery, immunohistochemis- try and ELISA methods were performed. The number of CD4+ and CD8+ T cells in breast cancer tissue was signifi-cantly increased compared with normal breast tissue with a clear predominance of CD8+ T cells. MIG/CXCL9 levels were significantly elevated respect normal breast tissue. This chemokine correlated with the number of CD8+ T cells only in non-metastatic tumors. These data suggest that MIG targets cytotoxic T cells. Their recruitment into breast car-cinoma can play a critical role in malignant progression, inhibiting the production of metastasis.Facultad de Ciencias Médicas (FCM

Lesiones precursoras : Detección de virus papiloma humano

El Virus del Papiloma Humano (VPH) ha sido hallado en lesiones benignas, potencialmente malignas y malignas de la mucosa bucal. Incluso se ha encontrado en mucosa bucal sana. Los tipos más frecuentes son el 6 y 11 de “bajo riesgo” y los considerados con potencial carcinogénico, 16, 18, 31, 33, 35, 45 y 51, entre otros. El objetivo de este trabajo fue hallar tipos virales de bajo y alto riesgo a través de un rastreo en una serie de lesiones precursoras de mucosa bucal.Resumen publicado en Libro de resúmenes. Producción en Investigación, Docencia, Gestión, Extensión. Universidad Nacional de Rosario: Rosario, 2009.Facultad de Odontología (FOLP

Lesiones precursoras : Detección de virus papiloma humano

El Virus del Papiloma Humano (VPH) ha sido hallado en lesiones benignas, potencialmente malignas y malignas de la mucosa bucal. Incluso se ha encontrado en mucosa bucal sana. Los tipos más frecuentes son el 6 y 11 de “bajo riesgo” y los considerados con potencial carcinogénico, 16, 18, 31, 33, 35, 45 y 51, entre otros. El objetivo de este trabajo fue hallar tipos virales de bajo y alto riesgo a través de un rastreo en una serie de lesiones precursoras de mucosa bucal.Resumen publicado en Libro de resúmenes. Producción en Investigación, Docencia, Gestión, Extensión. Universidad Nacional de Rosario: Rosario, 2009.Facultad de Odontología (FOLP

Lesiones precursoras : Detección de virus papiloma humano

El Virus del Papiloma Humano (VPH) ha sido hallado en lesiones benignas, potencialmente malignas y malignas de la mucosa bucal. Incluso se ha encontrado en mucosa bucal sana. Los tipos más frecuentes son el 6 y 11 de “bajo riesgo” y los considerados con potencial carcinogénico, 16, 18, 31, 33, 35, 45 y 51, entre otros. El objetivo de este trabajo fue hallar tipos virales de bajo y alto riesgo a través de un rastreo en una serie de lesiones precursoras de mucosa bucal.Resumen publicado en Libro de resúmenes. Producción en Investigación, Docencia, Gestión, Extensión. Universidad Nacional de Rosario: Rosario, 2009.Facultad de Odontología (FOLP

Relación entre proteasas y sus inhibidores en la progresión tumoral

La invasión y metástasis son acontecimientos claves en la progresión tumoral. Están implicadas entre otras moléculas, las metaloproteasas (MMPs) sintetizadas por las células estromales y tumorales.Son las principales mediadoras de las modificaciones observadas en el microambiente tumoral durante la progresión del cáncer. Su acción depende del equilibrio que mantienen con sus inhibidores endógenos TIMPs (Tissue Inhibitors of Metalloproteases).Facultad de Ciencias Médica