Relationship between platelet parameters and sudden sensorineural hearing loss: a systematic review and meta-analysis

Background: Sudden deafness or sudden sensorineural hearing loss (SSNHO is defined as sensorineural hearing loss of greater than 30 dB over 3 contiguous puretone frequencies occurring within 3 days' periodObjective: To investigate the relationship of some platelet parameters including platelet count (PC), mean platelet volume (MPV) and platelet distribution width (PDW) with the occurrence of SSNHL.Data source: A PubMed, Science Direct, Scopus, OVID, EMBASE and Google Scholar search (date last searchedApril2016) search was done. No restrictions of time, language and location were placed.Study selection: All case-control studies which have been studied the relationship of PC, MPV and PDW with the occurrence of SSNHL were included in the meta-analysis.Data extraction: The required data from selected studies including the title, authors, publication date, location of study, sample size of patients and control groups, number of withdrawals, the mean and standard deviation of PC, MPV and PDW for patients and control groups and the result of different tests were extracted and entered to EX CELL.Data synthesis: A total of 9 case-control studies were r found in our search from them 8 studies have reported mean PC, 7 studies have reported mean MPV and 4 studies have reported mean PDW. Our analysis showed that mean PC of patients is 0.03 (-0.14-0.20) unit higher than that of controls with 95% CI which is not statistically significant. Also, mean MPV of patients is 0.31 (-0.03-0.65) unit higher than that of controls with 95% CI which is statistically not significant too. Finally, mean PDW of patients is 0.70 (0.03- 1.37) unit higher than that of controls with 95% CI which is statistically significant.Conclusions: Our study confirmed only the probable relationship of PDW and SSNHL but due to the limited studies on this subject more studies is needed

Pathway based therapeutic targets identification and development of an interactive database CampyNIBase of <i>Campylobacter jejuni</i> RM1221 through non-redundant protein dataset

<div><p>The bacterial species <i>Campylobacter jejuni RM1221</i> (<i>CjR</i>) is the primary cause of campylobacteriosis which poses a global threat for human health. Over the years the efficacy of antibiotic treatment is becoming more fruitless due to the development of multiple drug resistant strains. Therefore, identification of new drug targets is a valuable tool for the development of new treatments for affected patients and can be obtained by targeting essential protein(s) of <i>CjR</i>. We conducted this <i>in silico</i> study in order to identify therapeutic targets by subtractive <i>CjR</i> proteome analysis. The most important proteins of the <i>CjR</i> proteome, which includes chokepoint enzymes, plasmid, virulence and antibiotic resistant proteins were annotated and subjected to subtractive analyses to filter out the <i>CjR</i> essential proteins from duplicate or human homologous proteins. Through the subtractive and characterization analysis we have identified 38 eligible therapeutic targets including 1 potential vaccine target. Also, 12 potential targets were found in interactive network, 5 targets to be dealt with FDA approved drugs and one pathway as potential pathway based drug target. In addition, a comprehensive database ‘CampyNIBase’ has also been developed. Besides the results of this study, the database is enriched with other information such as 3D models of the identified targets, experimental structures and Expressed Sequence Tag (EST) sequences. This study, including the database might be exploited for future research and the identification of effective therapeutics against campylobacteriosis. URL: (<a href="http://nib.portal.gov.bd/site/page/4516e965-8935-4129-8c3f-df95e754c562#Banner" target="_blank">http://nib.portal.gov.bd/site/page/4516e965-8935-4129-8c3f-df95e754c562#Banner</a>).</p></div

List of targets through identification channel.

<p>List of targets through identification channel.</p

The snapshot of the ‘CampyNIBase’ database.

<p>(a) Homepage tab: summary of <i>campylobacter jejuni</i> RM1221. (b) EST tab: EST related information. (c) Essential proteins tab: essential proteins of <i>campylobacter jejuni</i> RM1221. (d) Therapeutic targets tab: identified targets and prioritization. (e) Information of built and experimental 3D structure. (f) Model verification.</p

Architecture of ‘CampyNIBase’ database.

<p>The contents of developed database were categorized with different types of important relevant information.</p

Complete flowchart of whole subtractive analysis.

<p>The workflow is represented as flowchart showing subtractive analysis of the proteins in each step.</p

Outline of subtractive analysis and list of proteins.

<p>The enlisted proteins were prioritized (different symbol) from different phase of subtractive channel of analysis.</p

Characteristic features of pathway based drug targets.

<p>Characteristic features of pathway based drug targets.</p