Experimental evaluation of Chathusama Vati for its Anti-Diarrheal Activity

Background: Diarrhea is the condition of having three or more loose or liquid bowel movements per day. The loss of fluids through diarrhea can cause dehydration and electrolyte disturbances such as potassium deficiency or other salt imbalance. According to the World Health Organization, diarrhea affects 3-5 billion people per year worldwide and cause 5 million death per year. It is an important health problem in all age groups and is a major cause of death in socio-economical backward class of people. Aim and Objective: An experimental evaluation of Chathusama Vati for its anti-diarrheal activity. Methods: The experimental study was a three-group experimental study, conducted on 6 different rats on each group. Total 18 healthy albino rats weighing between 150 gm to 200gm will be taken and divided randomly into three groups; Rats were induced diarrhea with castor oil orally. Rats of group 1 will be administered with distilled water served as control group. Group 2 will be administered with Loperamide 5 mg/kg. Group 3 will be administered with Chathusama Vati (Humandose×0.018). Results: In the experimental study the result was showed as the Chathusama Vati has significant action in Diarrhea. Conclusion: From the experimental study the result it was found that Chathusama Vati showed mild ant- diarrheal activity

Experimental evaluation of Ardrakadi Ghritam for its Anti-microbial Activity

Background: Ardrakadi Ghrita mentioned in Sahasrayogam and its Anti-microbial activity was analyzed. Aim and objective: An experimental evaluation of Ardrakadi Ghrita for its Anti-microbial activity. Methods: Experimental evaluation of Ardrakadi Ghritam for its Anti-microbial activity against microbes such as Escherichia Coli and Klebsiella Pneumonia. Results: Determination of zone inhibition by Agar well diffusion method. For Test Organism are Escherichia Coli and Klebsiella Pneumonia. Inoculum: Cell suspension prepared from cultures grown on Trypsin broth adjusted to 1-2 x105cells/ml. Conclusion: From the experimental study the result it was found that Ardrakadi Ghrita not showed ant- microbial activity

Pharmaceutico-Analytical Study of Bhuvaneshwara Rasa

Background: Bhuvaneshwara Rasa is an Ayurvedic preparation mentioned in Bhaishajya Ratnavali used in the treatment of all types of Atisara (diarrhea). There is a lack of data regarding the standardization of pharmaceutical process and analytical profile of Bhuvaneshwara Rasa. Aim: To prepare Bhuvaneshwara Rasa and analyse it using various physicochemical parameters. Materials and methods: Bhuvaneshwara Rasa was prepared as per the guidelines mentioned in Ayurvedic Pharmacopoeia of India. During the pharmaceutical procedure, all the ingredients were mixed thoroughly and triturated with jala. The pharmaceutical and analytical parameters were compiled, and data was recorded. Results: Bhuvaneshwara Rasa after preparation showed increase of 10% yield. The values of physicochemical parameters of Bhuvaneshwara Rasa were as follows: pH 5.69, loss on drying 6.4%, acid insoluble ash 2.5, total ash 8.72%, hardness 3.5 kg, friability 0.1%.TLC band blue at 254nm revealed. Conclusion: Data generated from pharmaceutical, analytical studies and TLC can be used to develop a preliminary standard profile for the formulation Bhuvaneshwara Rasa

An experimental evaluation of the Lithotriptic Activity of Ayurvedic drug Kāśīśa Bhasma

Kāśīśa (Ferrous Sulphate, Fe2SO4.7H20), is among the most commonly used mineral drug in Ayurveda, and has been specifically indicated in Mutrashmari. Urolithiasis, (Mutrashmari in Ayurveda) is the third most common urinary system disorder globally with high recurrence rate. The present study is to determine the underlying mechanism of Kāśīśa Bhasma as lithotriptic drug in animal model. Ethylene glycol (0.75% v/v) induced urolithiasis model was used to study the lithotriptic activity of Kāśīśa Bhasma in Wistar albino rats. 30 rats were divided into five groups and were allocated interventions accordingly. Two trial drug groups were given single and double doses of Kāśīśa Bhasma (150mg/kg & 300mg/kg respectively), and were compared against the standard control group (Tab. Cystone 750mg/kg), administered for 14 days. Relevant biochemical assay and histopathological analysis was done and analysed. In vivo study revealed, Kāśīśa Bhasma administered at 300mg/kg b.w. was associated with better lithotriptic activity. The results showed significant reduction in calcium oxalate deposits in the kidneys, restoring the elevated values of serum Creatinine, BUN, uric acid and urine parameters like calcium, phosphate and oxalate while also maintaining optimal urine pH when compared to negative control. The lithotriptic activity of Kāśīśa Bhasma was found to be corresponding to that of standard drug Tab Cystone

Nagapashana Pishti - A Potent Cardio Tonic

Nagapashana (Serpentine), a hydrous silicate of Magnesium (Mg6 (Sio10) OH8), is an important mineral drug used in Ayurveda, often in the form of Pishti (fine powder of Nagapashana). It is a Hrudya Dravya (cardiotonic). It is particularly indicated in Hrud Dourbalyaa, a condition associated with weakness of cardiac muscles. Cardiovascular disease is a leading cause of death in developed and developing countries. The present study was conducted to evaluate the cardio tonic activity of Nagapashana Pishti on Wistar rats. In the in-vivo study, Doxorubicin (2mg/kg) for 7 days was used to induce cardiac damage and the cardio tonic effect of Nagapashana Pishti at a dose of 150 and 300 mg/kg.b.w, was compared with standard drug Digoxin. Biochemical assays like serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), lactate dehydrogenase (LDH), Total Cholesterol (TC), Triglyceride (TG) and Creatine kinase Monoenzyme B (CKMB) were done together with histopathology of heart tissue and ECG analysis. The in-vivo study revealed that Nagapashana Pishti (300mg/kg) was relatively more effective due to decreased QT and ST interval in ECG, significantly reduced levels of serum CKMB, SGOT, SGPT, TC and LDH and improvement in myocardial tissue

Not Available

Not AvailableImproved Samba Mahsuri (ISM) is a popular, high-yielding, bacterial blight resistant rice variety possessing medium-slender grain type. As ISM is highly susceptible to blast disease of rice, through the present study we have transferred two major blast resistance genes, Pi2 and Pi54 into the elite variety by marker-assisted backcross breeding. The two blast resistance genes were transferred to ISM through sets of backcrosses. In every backcross generation, PCR-based markers, specific for the blast resistance genes (Pi2 and Pi54) and bacterial blight resistance genes (Xa21, xa13 and xa5) were utilized for foreground selection, while a set of 144 parental polymorphic SSR markers were used for background selection and backcrossing was carried out until BC2 generation. A solitary BC2F1 plant possessing Pi2 or Pi54 along with Xa21, xa13 and xa5 and > 90% recovery of ISM genome was selected from the two sets of backcrosses were crossed and the intercross F1s (ICF1s) thus obtained were selfed to generate ICF2s. Homozygous ICF2 plants carrying all the five resistance genes were identified through markers and advanced through selfing till ICF5 generation by adopting pedigree method of selection. Three best lines at ICF5, possessing excellent resistance against bacterial blight and blast and closely resembling or superior to ISM in terms of grain quality: yield and agro-morphological traits have been identified and advanced for multi-location trials.ICAR-Indian Agricultural Research Institute with award Number: (F.No.F.3/CRPMB/Gen/2015-16/1714)Department of Science and Technology (DST), Government of India for the INSPIRE fellowshi