THE TREATMENT OF KOREAN TRADITIONAL MUSICAL ELEMENTS IN WESTERN MUSICAL COMPOSITION: A BRIEF ANALYSIS OF \u3cem\u3eFOLKSONG REVISITED\u3c/em\u3e FOR SOLO PIANO BY JEAN AHN

Jean Ahn (b. 1976) is one of the active Korean woman composers in the U.S. Ahn’s goal is to introduce her works in the U.S. by composing pieces that combine Korean musical elements with Western compositional techniques. The purpose of this study is to provide an introduction to and analysis of Folksong Revisited for solo piano by Jean Ahn. This work demonstrates how Jean Ahn integrates Korean traditional musical elements and Western musical compositional techniques. For better understanding of Ahn’s three Korean folksong arrangements in the Folksong Revisited, this document provides brief information about Korean traditional music and explores elements of it. This document also examines the folksong sources of each piece and Ahn’s compositional approaches to them, and then provides performance suggestions

Hypoxia-dependent mitochondrial fission regulates endothelial progenitor cell migration, invasion, and tube formation

Tumor undergo uncontrolled, excessive proliferation leads to hypoxic microenvironment. To fulfill their demand for nutrient, and oxygen, tumor angiogenesis is required. Endothelial progenitor cells (EPCs) have been known to the main source of angiogenesis because of their potential to differentiation into endothelial cells. Therefore, understanding the mechanism of EPC-mediated angiogenesis in hypoxia is critical for development of cancer therapy. Recently, mitochondrial dynamics has emerged as a critical mechanism for cellular function and differentiation under hypoxic conditions. However, the role of mitochondrial dynamics in hypoxia-induced angiogenesis remains to be elucidated. In this study, we demonstrated that hypoxia-induced mitochondrial fission accelerates EPCs bioactivities. We first investigated the effect of hypoxia on EPC-mediated angiogenesis. Cell migration, invasion, and tube formation was significantly increased under hypoxic conditions; expression of EPC surface markers was unchanged. And mitochondrial fission was induced by hypoxia time-dependent manner. We found that hypoxia-induced mitochondrial fission was triggered by dynamin-related protein Drp1, specifically, phosphorylated DRP1 at Ser637, a suppression marker for mitochondrial fission, was impaired in hypoxia time-dependent manner. To confirm the role of DRP1 in EPC-mediated angiogenesis, we analyzed cell bioactivities using Mdivi-1, a selective DRP1 inhibitor, and DRP1 siRNA. DRP1 silencing or Mdivi-1 treatment dramatically reduced cell migration, invasion, and tube formation in EPCs, but the expression of EPC surface markers was unchanged. In conclusion, we uncovered a novel role of mitochondrial fission in hypoxia-induced angiogenesis. Therefore, we suggest that specific modulation of DRP1-mediated mitochondrial dynamics may be a potential therapeutic strategy in EPC-mediated tumor angiogenesis

A pepper MSRB2 gene confer drought tolerance in rice through the protection of chloroplast-targeted genes

Background: The perturbation of the steady state of reactive oxygen species (ROS) due to biotic and abiotic stresses in a plant could lead to protein denaturation through the modification of amino acid residues, including the oxidation of methionine residues. Methionine sulfoxide reductases (MSRs) catalyze the reduction of methionine sulfoxide back to the methionine residue. To assess the role of this enzyme, we generated transgenic rice using a pepper CaMSRB2 gene under the control of the rice Rab21 (responsive to ABA protein 21) promoter with/without a selection marker, the bar gene. Results: A drought resistance test on transgenic plants showed that CaMSRB2 confers drought tolerance to rice, as evidenced by less oxidative stress symptoms and a strengthened PSII quantum yield under stress conditions, and increased survival rate and chlorophyll index after the re-watering. The results from immunoblotting using a methionine sulfoxide antibody and nano-LC-MS/MS spectrometry suggest that porphobilinogen deaminase (PBGD), which is involved in chlorophyll synthesis, is a putative target of CaMSRB2. The oxidized methionine content of PBGD expressed in E. coli increased in the presence of H2O2, and the Met-95 and Met-227 residues of PBGD were reduced by CaMSRB2 in the presence of dithiothreitol (DTT). An expression profiling analysis of the overexpression lines also suggested that photosystems are less severely affected by drought stress. Conclusions: Our results indicate that CaMSRB2 might play an important functional role in chloroplasts for conferring drought stress tolerance in rice.OAIID:oai:osos.snu.ac.kr:snu2014-01/102/0000005113/8SEQ:8PERF_CD:SNU2014-01EVAL_ITEM_CD:102USER_ID:0000005113ADJUST_YN:NEMP_ID:A077085DEPT_CD:517CITE_RATE:3.73FILENAME:msrb-김연기.pdfDEPT_NM:식물생산과학부EMAIL:[email protected]_YN:YCONFIRM:

Additional file 5: Figure S4. of Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice

The expression patterns of transcription factors as a putative target of OsSMF1 based on the 300 K Rice Genome Microarray ( www.ggbio.com ). The expression was measured in different sized panicles before heading (1, 3, 5, 8, 10, 15, 20, and 22 cm), at the indicated days after pollination (1, 3, 4, 11, and 21 days) and in the leaf, root, germinating seed, callus, and regenerating callus. (PPTX 75 kb

Plasmonic–Photonic Interference Coupling in Submicrometer Amorphous TiO₂–Ag Nanoarchitectures

In this study, we report the crystallinity effects of submicrometer titanium dioxide (TiO₂) nanotube (TNT) incorporated with silver (Ag) nanoparticles (NPs) on surface-enhanced Raman scattering (SERS) sensitivity. Furthermore, we demonstrate the SERS behaviors dependent on the plasmonic–photonic interference coupling (P-PIC) in the TNT-AgNP nanoarchitectures. Amorphous TNTs (A-TNTs) are synthesized through a two-step anodization on titanium (Ti) substrate, and crystalline TNTs (C-TNTs) are then prepared by using thermal annealing process at 500 °C in air. After thermally evaporating 20 nm thick Ag on TNTs, we investigate SERS signals according to the crystallinity and P-PIC on our TNT-AgNP nanostructures. (A-TNTs)-AgNP substrates show dramatically enhanced SERS performance as compared to (C-TNTs)-AgNP substrates. We attribute the high enhancement on (A-TNTs)-AgNP substrates with electron confinement at the interface between A-TNTs and AgNPs as due to the high interfacial barrier resistance caused by band edge positions. Moreover, the TNT length variation in (A-TNTs)-AgNP nanostructures results in different constructive or destructive interference patterns, which in turn affects the P-PIC. Finally, we could understand the significant dependency of SERS intensity on P-PIC in (A-TNTs)-AgNP nanostructures. Our results thus might provide a suitable design for a myriad of applications of enhanced EM on plasmonic-integrated devices

Additional file 1: Figure S1. of Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice

Transcript levels of Wsi18, OsSMF1, and OsREM from 300 K Rice Genome Microarray ( www.ggbio.com ). The transcript levels were measured in different sized panicles before heading (1, 3, 5, 8, 10, 15, 20, and 22 cm), at the indicated days after pollination (1, 3, 4, 11, and 21 days) and in the leaf, root, germinating seed, callus, and regenerating callus. (PPTX 70 kb

Additional file 4: Figure S3. of Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice

The query gene, OsSMF1, is marked by an asterisk. Each circle indicates a gene, and the lines represent the correlations between the genes. Eighty-five genes were identified as OsSMF1-related genes, with a minimum correlation value of 0.55 and depth of 1. (PPTX 473 kb

RapaNet: A Web Tool for the Co-Expression Analysis of Brassica rapa Genes

Accumulated microarray data are used for assessing gene function by providing statistical values for co-expressed genes; however, only a limited number of Web tools are available for analyzing the co-expression of genes of Brassica rapa . We have developed a Web tool called RapaNet ( http://bioinfo.mju.ac.kr/arraynet/brassica300k/query/ ), which is based on a data set of 143 B rapa microarrays compiled from various organs and at different developmental stages during exposure to biotic or abiotic stress. RapaNet visualizes correlated gene expression information via correlational networks and phylogenetic trees using Pearson correlation coefficient ( r ). In addition, RapaNet provides hierarchical clustering diagrams, scatterplots of log ratio intensities, related pathway maps, and cis -element lists of promoter regions. To ascertain the functionality of RapaNet, the correlated genes encoding ribosomal protein (L7Ae), photosystem II protein D1 (psbA), and cytochrome P450 monooxygenase in glucosinolate biosynthesis (CYP79F1) were retrieved from RapaNet and compared with their Arabidopsis homologues. An analysis of the co-expressed genes revealed their shared and unique features

Detection of recombinant proteins containing methionine sulfoxide (MetSO) residues by western blotting.

<p>Rubi (Ribulose-bisphosphate carboxylase activase, Os11g0707000), PBGD (Porphobilinogen deaminase, Os02g0168800), and Cys (Cysteine synthase, Os12g0625000) were cloned into the pET-DsRed vector, expressed in <i>E. coli</i>, purified, and treated with 0.3% H₂O₂. These recombinant proteins containing MetSO were detected by western blotting with the methionine sulfoxide polyclonal antibody (Cayman). kDa, molecular mass indicators (in kDa The experiments were representative of three independent experiments.</p

MetSO/Met ratio in individual methionine residues of PBGD.

<p>The gray and black bars represent the Met and MetSO residues, respectively. The MetSO content is expressed as the percent of MetSO in relation to the each Met residues (Met + MetSO). The figures that shown are in the bar graph represent the absolute numbers of Met or MetSO. The results shown are the mean ± SD, n = 3 replicates for each group (*<i>P</i><0.05 compared to PBGD + H₂O₂ + MSRB2 value).</p