11 research outputs found
Structural and electrical studies of sodium iodide doped poly(vinyl alcohol) polymer electrolyte films for their application in electrochemical cells
Improved Haacke’s quality factor and paramagnetic-to-ferromagnetic transition realized in Ni co-doped CdO:Zn thin films
Amelioration effects against N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Swiss albino rats by whole plant extract of Achyranthes aspera
Objective: The prevalence of oxidative stress may be implicated in the
etiology of many pathological conditions. Protective antioxidant action
imparted by many plant extracts and plant products make them a
promising therapeutic drug for free-radical-induced pathologies. In
this study, we assessed the antioxidant potential and suppressive
effects of Achyranthes aspera by evaluating the hepatic diagnostic
markers on chemical-induced hepatocarcinogenesis. Materials and
Methods: The in vivo model of hepatocarcinogenesis was studied in Swiss
albino rats. Experimental rats were divided into five groups: control,
positive control (NDEA and CCl 4 ), A. aspera treated (100, 200, and
400 mg/kg b.w.). At 20 weeks after the administration of NDEA and CCl 4
, treated rats received A. aspera extract (AAE) at a dose of 100, 200,
and 400 mg/kg once daily route. At the end of 24 weeks, the liver and
relative liver weight and body weight were estimated. Lipid
peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT),
glutathione peroxidase (GPx), glutathione-S-transferase (GST), and
reduced glutathione (GSH) were assayed. The hepatic diagnostic markers
namely serum glutamic oxaloacetic transminase (AST), serum glutamic
pyruvate transminase (ALT), serum alkaline phosphatase (ALP), gamma
glutamyl transpeptidase (GGT), and bilirubin (BL) were also assayed,
and the histopathological studies were investigated in control,
positive control, and experimental groups. Results: The extract did not
show acute toxicity and the per se effect of the extract showed
decrease in LPO, demonstrating antioxidant potential and furthermore no
change in the hepatic diagnosis markers was observed. Administration of
AAE suppressed hepatic diagnostic and oxidative stress markers as
revealed by decrease in NDEA and CCl 4 -induced elevated levels of
SGPT, SGOT, SALP, GGT, bilirubin, and LPO. There was also a significant
elevation in the levels of SOD, CAT, GPx, GST, and GSH as observed
after AAE treatment. The liver and relative liver weight were decreased
after treatment with AAE in comparison to positive control group. The
architecture of hepatic tissue was normalized upon treatment with
extract at different dose graded at 100, 200, and 400 mg/kg. b.w. in
comparison to positive control group. Conclusion: These results suggest
that A. aspera significantly alleviate hepatic diagnostic and oxidative
stress markers which signify its protective effect against NDEA and CCl
4 -induced two-stage hepatocarcinogenesis