Sertolinoma maligno en un canino criptórquido con hiperplasia prostática benigna quística: reporte de caso

The case of an 8-year-old Beagle canine, who was attended at the ASMEVET veterinary hospital in the city of Tunja, Colombia, is presented. The owner reported that in recent months he has been losing hair, has increased water consumption, has not been neutered, and more recently is depressed, lack of appetite and with signs of dysuria. On physical examination, generalized alopecia and gynecomastia were observed, and the prostate was enlarged and asymmetric. Transabdominal ultrasonography revealed an enlarged prostate, with heteroechogenic parenchyma and microcysts, and the testicles were retained at the level of the apex of the bladder. Enrofloxacin was administered for 5 days, and meloxicam for 4 days, and then the patient underwent celiotomy to remove the retained testicles. The histopathological study reported malignant sertolinoma. Fine needle aspiration of prostate tissue revealed cells consistent with benign prostatic hyperplasia. After 90 days of bilateral orchiectomy, remission of alopecia was observed and ultrasound evaluation showed a decrease in the size of the prostate.Se presenta el caso de un canino de 8 años, raza Beagle, que fue atendido en el hospital veterinario ASMEVET de la ciudad de Tunja, Colombia. El propietario informó que en los últimos meses ha estado perdiendo, ha aumentado el consumo de agua, no ha sido castrado, y que recientemente se encuentra deprimido, inapetente y con signos de disuria. Al examen físico se observa alopecia generalizada y ginecomastia, y la próstata se encuentra aumentada de tamaño y asimétrica. La ultrasonografía transabdominal revela próstata aumentada de tamaño, con parénquima heteroecogénico y microquistes, y los testículos se encontraban retenidos a la altura del vértice de la vejiga. Se administró enrofloxacina por 5 días, y meloxicam por 4 días, y luego se sometió a celiotomía para extirpar los testículos retenidos. El estudio histopatológico reportó sertolinoma maligno. La muestra del tejido prostático mediante aspiración con aguja fina reveló células compatibles con hiperplasia prostática benigna. Después de los 90 días de la orquiectomía bilateral se observó la remisión de la alopecia y la ecografía control mostró disminución del tamaño de la próstata

Establishing an in vitro production program for buffalo embryos (Bubalus bubalis) in Colombia

Objective. Evaluate the results of the standardization of the in vitro production program of buffalo embryos, using oocytes obtained by ultrasound guided oocyte puncture during the 2012 breeding season in Colombia. Materials and methods. Fifty seven buffalo females were selected for ultrasound guided transvaginal aspiration of follicles, oocytes were identified within follicular fluid, classified and transported to the laboratory and matured in vitro for 18 to 20 hours. Frozen semen of seven Mediterranean bulls were used, motile sperm was obtained using the Percoll technique and oocytes were inseminated with 2 million sperm/ml. Presumptive zygotes were cultured for 6 days, grade 1 embryos obtained were frozen using ethylene glycol. Embryos were transferred to females on day 5 during natural cycle. Results. 97 aspirations were performed on the 57 animals, in 8.2% of the aspirations no oocytes were found. 8 oocytes/aspiration were obtained. Of the 783 oocytes, 92% were classified as viable (721/783) and were fertilized. The cleavage and blastocyst rate were 23% and 19% respectively. 37 embryos were transferred and 11 pregnancies were obtained, confirmed by rectal palpation 60 days after transfer, with a pregnancy rate of 29.7%. Conclusions. The results presented here are comparable with others in literature and shows the feasibility of producing in vitro embryos and pregnancies after the standardization of current protocols, with normal and sexed semen and transfer during natural cycle in buffalo.Key words: Buffaloes, embryo, fertilization in vitro, reproduction (Source: MeSH)

Establishing an in vitro production program for buffalo embryos (Bubalus bubalis) in Colombia

Objective. Evaluate the results of the standardization of the in vitro production program of buffalo embryos, using oocytes obtained by ultrasound guided oocyte puncture during the 2012 breeding season in Colombia. Materials and methods. Fifty seven buffalo females were selected for ultrasound guided transvaginal aspiration of follicles, oocytes were identified within follicular fluid, classified and transported to the laboratory and matured in vitro for 18 to 20 hours. Frozen semen of seven Mediterranean bulls were used, motile sperm was obtained using the Percoll technique and oocytes were inseminated with 2 million sperm/ml. Presumptive zygotes were cultured for 6 days, grade 1 embryos obtained were frozen using ethylene glycol. Embryos were transferred to females on day 5 during natural cycle. Results. 97 aspirations were performed on the 57 animals, in 8.2% of the aspirations no oocytes were found. 8 oocytes/aspiration were obtained. Of the 783 oocytes, 92% were classified as viable (721/783) and were fertilized. The cleavage and blastocyst rate were 23% and 19% respectively. 37 embryos were transferred and 11 pregnancies were obtained, confirmed by rectal palpation 60 days after transfer, with a pregnancy rate of 29.7%. Conclusions. The results presented here are comparable with others in literature and shows the feasibility of producing in vitro embryos and pregnancies after the standardization of current protocols, with normal and sexed semen and transfer during natural cycle in buffalo.Objetivo. Evaluar los resultados de la estandarización de la técnica de producción in vitro de embriones de búfalo, a partir de oocitos obtenidos por punción folicular durante la estación reproductiva del 2012 en una hacienda en Cordoba, Colombia. Materiales y métodos. Cincuenta y siete búfalas fueron seleccionadas para aspiración transvaginal de folículos guiada por ultrasonido, los oocitos fueron identificados y madurados in vitro. Se utilizó semen congelado de 7 búfalos de la raza Mediterráneo para la fertilización in vitro. La fracción móvil fue separada en un gradiente de Percoll, los oocitos fueron inseminados con 2 millones de espermatozides/mL, los presuntos cigotos fueron cultivados por 6 días y los embriones grado 1 obtenidos fueron congelados utilizando etilenglicol. Posteriormente, los embriones fueron transferidos el día 5 post estro en búfalas en ciclo natural. Resultados. En las 57 búfalas, se realizaron 97 aspiraciones foliculares, no se obtuvieron oocitos en el 8.2% de los procedimientos. Se obtuvieron en promedio 8 oocitos por búfala en cada sesión de aspiración. Se recolectaron 783 oocitos de los cuales fueron clasificados como viables el 92% (721/783), se obtuvo una taza de clivaje del 23% y de blastocistos del 19%. De 37 embriones transferidos se obtuvieron 11 preñeces, confirmadas por palpación rectal a los 60 días postransferencia, obteniéndose una taza de preñez del 29.7% Conclusiones. Los resultados presentados en este trabajo son comparables con los de la literatura, en la cual se muestra cómo es posible obtener embriones de semen convencional y sexado, además de producir gestaciones con protocolos estándar de fertilización in vitro y transferencia en ciclos con celo natural adaptados para la especie