Effect of Mitomycin - C and Triamcinolone on Preventing Urethral Strictures

Urethral stricture is a common disease with high recurrence rate. Several manipulations were defined to prevent the recurrence but the results were disappointing. This study aimed to evaluate the efficacy of triamcinolone and mitomycin-C on urethral stricture formation and their effect on inhibition of urethral fibrosis. A total of 24 New Zealand rabbits were divided into 3 groups. Urethras of rabbits were traumatized with pediatric resectoscope. Resection area was irrigated with 10mL saline, swapped with a cotton wool soaked with 0.5mg/mL MMC and injected by 40mg triamcinolone in groups 1, 2 and 3 respectively. Retrograde urethrogram was performed at 28th day of procedure and the urethra was removed for histopathologic evaluation. There were significant differences in urethral diameters and in lumen reduction rate between the control and study groups (p< 0.001). Compared to control group, all treatment groups showed mild fibrosis, less collagen bundle irregularity, and lower numbers of fibroblasts (p= 0.003). The Tunnel assay showed that the number of apoptotic cells in the submucosal connective tissue was quantitatively higher in control groups (p= 0.034). In the view of efficacy and safety, MMC and triamcinolone have the potential to replace the use of stents, clean intermittent catheterization, or long term catheters following internal urethrotomy. There were no statistically significant differences between two agents in terms of preventing urethral stricture formation in the present study. Mitomycin C and triamcinolone decreased the recurrence rates of urethral stricture

Investigation on malondialdehyde, s100b and advanced oxidation protein product levels in significant hyperbilirubinemia and the effect of intensive phototherapy on these parameter

Background: The parameters of oxidative stress [advanced oxidation protein products (AOPPs), malondialdehyde (MDA), and S100B] and the effect of intensive phototherapy (PT) on these parameters have not been studied extensively in newborns with significant hyperbilirubinemia (SH). We aimed to measure the levels of MDA, S100B, and AOPPs in newborns with SH, and to compare newborns with healthy control newborns without hyperbilirubinemia on the basis of these parameters of oxidative stress. In addition, we investigated the effect of intensive PT on these parameters during the treatment of SH and report our findings for the first time in the literature. Methods: The study was performed in newborns (n = 62) who underwent intensive PT because of SH. Newborns without jaundice constituted the control group (n = 30). Both groups were compared with respect to demographic characteristics and biochemical (laboratory) parameters including MDA, AOPPs, and S100B. MDA, AOPPs, and S100B were also compared before and after intensive PT in the PT group. In the study group, a correlation analysis of demographic characteristics; MDA, AOPP, and S100B values; and changes occurring in MDA, AOPPs, and S100B values due to the effect of intensive PT was performed. Results: Serum total bilirubin, S100B, and MDA levels in the PT group before performing PT were significantly higher than those in the control group. In newborns receiving PT serum total bilirubin, MDA and AOPP levels decreased significantly after intensive PT. In correlation analysis, a statistically significant negative correlation was found only between the amount of billrubin decrease with PT and AOPP levels after PT in the study group. Conclusion: Whether the significant decrease in MDA levels, which was higher prior to PT, is due to the decrease in serum bilirubin levels or due to the effect of intensive PT itself remains to be determined in further studies. The decrease in AOPP levels after PT implies that intensive PT has protective effects on oxidative stress. Copyright (C) 2014, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC. All rights reserved

İdrar Numunelerinde Preanalitik Hata Kaynaklarının Azaltılması

Urine may be a waste product, but it contains an enormous amount ofinformation. Well-standardized procedures for collection, transport,sample preparation and analysis should become the basis of an effective diagnostic strategy for urinalysis. As reproducibility of urinalysis hasbeen greatly improved due to recent technological progress, preanalytical requirements of urinalysis have gained importance and have becomestricter. Since the patients themselves often sample urine specimens,urinalysis is very susceptible to preanalytical issues. In order to increasethe reliability of the test results and to reduce the cost, the precautionarymeasure and prevention is very important in the preanalytical phase. Thelaboratory must monitor and evaluate the overall quality of the preanalytic systems and correct identified problems for each specialty and subspecialty of testing performed. The laboratory must establish and followwritten policies and procedures for an ongoing mechanism to monitorassess, and when indicated, correct problems identified in the preanalytic systems. The preanalytic systems assessment must include a review ofthe effectiveness of corrective actions taken to resolve problems, revisionof policies and procedures necessary to prevent recurrence of problems,and discussion of preanalytic systems assessment reviews with appropriate staff. The laboratory must document all preanalytic systems assessment activities. An effective diagnostic strategy from urine should bebased on standard procedures for collection, transport and analysis. Thetime of specimen collection must be recorded both on the examinationrequest and on the subsequent report to aid in the correct interpretationof findings.</p

Investigation of adenosine deaminase activity in patients with mycosis fungoides

Background and Design: Mycosis fungoides (ME) is a cutaneous T cell lymphoma. The clinical and histopathological diagnosis of early mycosis fungoides is usually difficult. There is no special laboratory method for the diagnosis of ME and this is the most important problem in diagnosis and follow up the effectiveness of treatment. Adenosine deaminase (ADA) activity is a non-specific marker of T cell activation. In the present study, we aimed to investigate the levels of plasma and tissue ADA in patients with mycosis fungoides and to determine if ADA is an activation criterion for this disease

EVALUATION OF THE EFFECT OF VITAMIN D SUPPLEMENT ON THE LEVEL OF MMP-9 IN GINGIVAL CREVICULAR FLUID IN PERIODONTAL DISEASES

AIM: The aim of this study was to evaluate the effect of vitamin D supplementation on the levels of the gingival crevicular fluid (GCF) MMP-9, among both patients with periodontal disease (gingivitis and periodontitis) and periodontal healthy individuals who have a vitamin D deficiency.MATERIAL AND METHODS: This study is a follow-up to the research project that evaluated the relationship between serum vitamin D concentration and MMP-9 levels in gingival crevicular fluid in periodontal diseases.In this particular study, the impact of vitamin D supplementation was assessed by analyzing the levels of MMP- 9 in the gingival crevicular fluid collected from the same dental sites of patients with vitamin D deficiencywho received appropriate medical treatment and had been using vitamin D supplements for one month. The levels of MMP-9 were compared between the baseline of the study and after vitamin D supplementation.A total of 101 participants were included in this study, consisting of 34 with periodontitis, 34 with gingivitis, and 33 periodontally healthy individuals. The participants’ periodontal status was evaluated radiographicallyand clinically by measuring probing depth, clinical attachment loss, gingival index, plaque index, and radiographic analysis. The levels of MMP-9 in the gingival crevicular fluid were analyzed using the enzymelinkedimmunosorbent assay (ELISA) method.”RESULTS: The results of our study suggest that the risk of periodontal disease may be elevated due to vitamin D deficiency, which could be attributed to the effect on MMP9 expression.CONCLUSIONS: This study will provide a better understanding of the role of vitamin D in the pathogenesis of periodontitis and could lead to the development of new therapeutic approaches for the management of thischronic inflammatory periodontal diseases.</p