Efficacy of MRI in the differential diagnosis of odontogenic keratocyst: literature review

According to the World Health Organization (WHO 2017), the odontogenic keratocyst (OKC) is classified as an odon­togenic developmental cyst, with origins from the cellular remnants of the dental lamina. The characteristics of a high rate of cell proliferation, relapse and aggressive growth guide the choice of the type of surgical treatment for the lesion and, consequently, the prognosis. The use of Magnetic Resonance Imaging (MRI) for the differential diagnosis of odon­togenic lesions does not replace anatomopathological examination, but the types of protocols already described illustra­te the influence of these different protocols on the qualitative and quantitative description of keratocysts. We conclude that magnetic resonance imaging is valid as a tool to aid diagnosis of odontogenic lesions, especially for differential diagnosis studies between odontogenic keratocysts and ameloblastomas. De acordo com a Organização Mundial de Saúde (OMS, 2017), o ceratocisto odontogênico (CO) é classificado como um cisto odontogênico de desenvolvimento, com origem nos restos celulares da lâmina dentária. A alta taxa de proliferação celular, recaída e crescimento agressivo direciona a escolha do tipo de tratamento cirúrgico para a lesão e, consequentemente, para seu prognóstico. O uso da ressonância magnética (RM) para o diagnóstico diferencial das lesões odontogênicas não substitui os exames anatomopatológicos, mas os tipos de protocolos já descritos il­ustram a influência desses diferentes protocolos na descrição quantitativa e qualitativa dos ceratocistos. Concluímos que a ressonância magnética é válida como ferramenta de auxílio para o diagnóstico de lesões odontogênicas, principalmente em estudos de diagnóstico diferencial entre ceratocistos odontogênicos e ameloblastomas

The influence of film type and processing method on radiographic density for occlusal caries diagnosis

Objectives:     This study aimed to investigate the influence of the radiographic processing method on the radiographic density of two types of film.  Radiographs from non-cavitated occlusal caries lesions obtained in a previous study were used. Methods: We analyzed 28 films from 12 patients that were obtained with the same X-ray device. Seven Ektaspeed (Kodak Eastman Kodak Co, Rochester, NY, USA) and seven Ultraspeed (Kodak Eastman Kodak Co) films were manually processed, while seven Ektaspeed and seven Ultraspeed films were automatically processed. The angle of incidence of the X-ray beam and the distances between the apparatus, the tooth and the film were standardized. Therefore, the study comprised four groups: GE1: Ektaspeed manually processed; GE2: Ektaspeed automatically processed; GU1: Ultraspeed manually processed; and GU2: Ultraspeed automatically processed. The images were digitized (G40450 Scan Jet C/T, Hewlett-Packard) and analyzed using Digora for Windows 2.7 (Soredex Medical Systems) to obtain the average radiographic density, and the data were analyzed using the Student’s t test for paired data (Bioestat 5.3).  Results: Only the Ektaspeed film showed statistically significant differences between radiographic densities with different processing methods (p = 0.0037).  Conclusions: The Ektaspeed film automatic pocessed is better suited for the early diagnosis of caries lesions.

Eficácia da desinfecção com álcool 70% (p/v) de superfícies contaminadas sem limpeza prévia

OBJECTIVE: To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD: An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS: There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load ≤ 102 CFU. CONCLUSION: The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces.OBJETIVO: Evaluar la eficacia desinfectante de alcohol al 70% (p/v) por fricción, sin limpieza previa, en las superficies de trabajo, como procedimiento de desinfección cotidiana o de rutina en Servicios de Salud. MÉTODO: Fue desarrollado un estudio experimental de laboratorio, aleatorio y uni-ciego. Las muestras fueron obtenidas de superficies esmaltadas, intencionalmente contaminadas con microorganismos Serratia marcescens ATCC 14756 106 UFC/mL acrecido con 10% de saliva humana, sometidas al procedimiento de desinfección SIN limpieza previa. Los resultados fueron comparados a la desinfección después de limpieza. RESULTADOS: Hubo una reducción de seis logaritmos de la población microbiana inicial, igualmente en los grupos COM y SIN limpieza previa (p=0,440) y una carga microbiana residual ≤ 102 UFC. CONCLUSIÓN: La investigación demostró que es aceptable la práctica evaluada ofreciendo así una importante respuesta para el área de la salud, especialmente para la Enfermería, que es la que más ejecuta procedimientos de limpieza/desinfección cotidiana o de rutina de esas superficies de trabajo.OBJETIVO: avaliar a eficácia desinfetante do álcool 70% (p/v) sob fricção, sem limpeza prévia, nas superfícies de trabalho, como procedimento de desinfecção concorrente em Serviços de Saúde. MÉTODO: foi desenvolvido estudo experimental laboratorial, randomizado e unicegado. As amostras foram constituídas de superfícies esmaltadas, intencionalmente contaminadas com microrganismos Serratia marcescens ATCC 14756 106 Unidades Formadoras de Colônias/mL, acrescidos de 10% de saliva humana, e submetidas ao procedimento de desinfecção sem limpeza prévia. Os resultados foram comparados à desinfecção precedida da limpeza. RESULTADOS: houve redução de seis logaritmos da população microbiana inicial, igualmente nos grupos com e sem limpeza prévia (p=0,440) e uma carga microbiana residual ≤ 102 Unidades Formadoras de Colônias. CONCLUSÃO: a pesquisa demonstrou a aceitabilidade da prática avaliada, trazendo importante resposta para a área da saúde, especialmente à enfermagem, que mais executa os procedimentos de limpeza/desinfecção concorrentes dessas superfícies de trabalho

Association of laser phototherapy with PRP improves healing of bisphosphonate-related osteonecrosis of the jaws in cancer patients: A preliminary study

The aim of this study was to compare retrospectively the effect of three different treatments on the healing outcome of bisphosphonate-related osteonecrosis of the jaws (BRONJ) in cancer patients. Twenty-two cancer patients were treated for BRONJ with one of the following protocols: clinical (pharmacological therapy), surgical (pharmacological plus surgical therapy), or PRP plus LPT (pharmacological plus surgical plus platelet rich plasma (PRP) plus laser phototherapy (LPT). The laser treatment was applied with a continuous diode laser (InGaAlP, 660 nm) using punctual and contact mode, 40 mW, spot size 0.042 cm(2), 6 J/cm(2) (6 s) and total energy of 0.24 J per point. The irradiations were performed on the exposed bone and surrounding soft tissue. The analysis of demographic data and risk factors was performed by gathering the following information: age, gender, primary tumor, bisphosphonate (BP) used, duration of BP intake, history of chemotherapy, use of steroids, and medical history of diabetes. The association between the current state of BRONJ (with or without bone exposure) and other qualitative variables was determined using the chi-square or Fisher's exact test. In all tests, the significance level adopted was 5%. Most BRONJ lesions occurred in the mandible (77%) after tooth extraction (55%) and in women (72%). A significantly higher percentage of patients reached the current state of BRONJ without bone exposure (86%) in the PPR plus LPT group than in the pharmacological (0%) and surgical (40%) groups after 1-month follow-up assessment. These results suggest that the association of pharmacological therapy and surgical therapy with PRP plus LPT significantly improves BRONJ healing in oncologic patients. Although prospective studies with larger sample sizes are still needed, this preliminary study may be used to inform a better-designed future study. (C) 2011 Elsevier Ltd. All rights reserved

Effectiveness of disinfection with alcohol 70% (w/v) of contaminated surfaces not previously cleaned

OBJECTIVE: To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD: An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS: There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load ≤ 102 CFU. CONCLUSION: The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces

Effectiveness of disinfection with alcohol 70% (w/v) of contaminated surfaces not previously cleaned

OBJECTIVE: To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD: An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS: There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load ≤ 102 CFU. CONCLUSION: The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces

Effectiveness of disinfection with alcohol 70% (w/v) of contaminated surfaces not previously cleaned Eficacia de la desinfección con alcohol al 70% (p/v) de superficies contaminadas sin limpieza previa Eficácia da desinfecção com álcool 70% (p/v) de superfícies contaminadas sem limpeza prévia

OBJECTIVE: To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD: An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS: There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load &#8804; 102 CFU. CONCLUSION: The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces.<br> OBJETIVO: Evaluar la eficacia desinfectante de alcohol al 70% (p/v) por fricci&#243;n, sin limpieza previa, en las superficies de trabajo, como procedimiento de desinfecci&#243;n cotidiana o de rutina en Servicios de Salud. M&#201;TODO: Fue desarrollado un estudio experimental de laboratorio, aleatorio y uni-ciego. Las muestras fueron obtenidas de superficies esmaltadas, intencionalmente contaminadas con microorganismos Serratia marcescens ATCC 14756 106 UFC/mL acrecido con 10% de saliva humana, sometidas al procedimiento de desinfecci&#243;n SIN limpieza previa. Los resultados fueron comparados a la desinfecci&#243;n despu&#233;s de limpieza. RESULTADOS: Hubo una reducci&#243;n de seis logaritmos de la poblaci&#243;n microbiana inicial, igualmente en los grupos COM y SIN limpieza previa (p=0,440) y una carga microbiana residual &#8804; 102 UFC. CONCLUSI&#211;N: La investigaci&#243;n demostr&#243; que es aceptable la pr&#225;ctica evaluada ofreciendo as&#237; una importante respuesta para el &#225;rea de la salud, especialmente para la Enfermer&#237;a, que es la que m&#225;s ejecuta procedimientos de limpieza/desinfecci&#243;n cotidiana o de rutina de esas superficies de trabajo.<br> OBJETIVO: avaliar a efic&#225;cia desinfetante do &#225;lcool 70% (p/v) sob fric&#231;&#227;o, sem limpeza pr&#233;via, nas superf&#237;cies de trabalho, como procedimento de desinfec&#231;&#227;o concorrente em Servi&#231;os de Sa&#250;de. M&#201;TODO: foi desenvolvido estudo experimental laboratorial, randomizado e unicegado. As amostras foram constitu&#237;das de superf&#237;cies esmaltadas, intencionalmente contaminadas com microrganismos Serratia marcescens ATCC 14756 106 Unidades Formadoras de Col&#244;nias/mL, acrescidos de 10% de saliva humana, e submetidas ao procedimento de desinfec&#231;&#227;o sem limpeza pr&#233;via. Os resultados foram comparados &#224; desinfec&#231;&#227;o precedida da limpeza. RESULTADOS: houve redu&#231;&#227;o de seis logaritmos da popula&#231;&#227;o microbiana inicial, igualmente nos grupos com e sem limpeza pr&#233;via (p=0,440) e uma carga microbiana residual &#8804; 102 Unidades Formadoras de Col&#244;nias. CONCLUS&#195;O: a pesquisa demonstrou a aceitabilidade da pr&#225;tica avaliada, trazendo importante resposta para a &#225;rea da sa&#250;de, especialmente &#224; enfermagem, que mais executa os procedimentos de limpeza/desinfec&#231;&#227;o concorrentes dessas superf&#237;cies de trabalho

Effectiveness of disinfection with alcohol 70% (w/v) of contaminated surfaces not previously cleaned

OBJECTIVE: To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD: An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS: There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load ≤ 102 CFU. CONCLUSION: The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces

Physical and Biological Properties of a Chitosan Hydrogel Scaffold Associated to Photobiomodulation Therapy for Dental Pulp Regeneration: An In Vitro and In Vivo Study

Background. The regeneration of dental pulp, especially in cases of pulp death of immature teeth, is the goal of the regenerative endodontic procedures (REPs) that are based on tissue engineering principles, consisting of stem cells, growth factors, and scaffolds. Photobiomodulation therapy (PBMT) showed to improve dental pulp regeneration through cell homing approaches in preclinical studies and has been proposed as the fourth element of tissue engineering. However, when a blood clot was used as a scaffold in one of these previous studies, only 30% of success was achieved. The authors pointed out the instability of the blood clot as the regeneration shortcoming. Then, to circumvent this problem, a new scaffold was developed to be applied with the blood clot. The hypothesis of the present study was that an experimental injectable chitosan hydrogel would facilitate the three-dimensional spatial organization of endogenous stem cells in dental pulp regeneration with no interference on the positive influence of PBMT. Methods. For the in vitro analysis, stem cells from the apical papilla (SCAPs) were characterized by flow cytometry and applied in the chitosan scaffold for evaluating adhesion, migration, and proliferation. For the in vivo analysis, the chitosan scaffold was applied in a rodent orthotopic dental pulp regeneration model under the influence of PBMT (660 nm; power output of 20 mW, beam area of 0.028 cm2, and energy density of 5 J/cm2). Results. The scaffold tested in this study allowed significantly higher viability, proliferation, and migration of SCAPs in vitro when PBMT was applied, especially with the energy density of 5 J/cm2. These results were in consonance to those of the in vivo data, where pulp-like tissue formation was observed inside the root canal. Conclusion. Chitosan hydrogel when applied with a blood clot and PBMT could in the future improve previous results of dental pulp regeneration through cell homing approaches