24 research outputs found

    Genomic Constitution of \u3cem\u3eFestulolium\u3c/em\u3e Varieties

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    Hybrids between species of ryegrass (Lolium) and fescue (Festuca) combine useful agronomical characteristics such as rapid establishment from seed and fodder quality from ryegrass and tolerance against abiotic and biotic stressses from fescue. The superior potential of hybrids has stimulated breeding programs generating so called Festulolium varieties. While the varieties have been evaluated extensively for their agronomic characteristics, little information is publicly available on their genomic constitution. The aim of our study was to analyse genomic constitution of a representative set of commercially available European Festulolium cultivars. To do this, we have employed genomic in situ hybridization (GISH)

    Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9

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    NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease

    Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)

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    Focal segmental glomerulosclerosis (FSGS) is a major cause of familial nephrotic syndrome. We generated 20 induced pluripotent stem cell lines from patients diagnosed with FSGS. The iPSC lines include 8 female and 12 male lines and cover a donor age range from 31 to 78. The lines were generated from peripheral blood mononuclear cells by integration-free reprogramming using Sendai virus vectors. Cell lines were fully characterized regarding their pluripotency and differentiation potential, and quality controlled for karyotypic integrity, identity and clearance of reprogramming vectors. The generated cell lines represent a valuable tool for disease modelling and drug development for FSGS

    Extensive Performance Studies for the ATLAS BIS-MDT Precision Muon Chambers with Cosmic Rays

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    ATLAS (a toroidal LHC apparatus) is a general purpose experiment that will start its operation at the large hadron collider (LHC) at CERN in 2007. The ATLAS detector is designed to explore numerous physics processes by recording, measuring, and investigating the products emerging from proton-proton collisions at energies up to 14 TeV. High-precision muon momentum measurement (dp/p ∼ 10%at pT = 1 TeV/c) over large areas using monitored drift tube (MDT) chambers is crucial for the ATLAS experiment. More than 1200 MDT chambers, consisting of approximately 370 000 drift tubes, will provide a total coverage of 5500 m2. Three Greek universities have taken the responsibility to construct 130 barrel inner small (BIS)-MDT chambers using 30 000 drift tubes of ∼ 1.7m length that have been quality tested before assembly. The design of the muon drift tubes aims at high detection efficiency (> 95%) and a spatial single tube resolution of < 80 μm. This paper describes the cosmic ray test setup, which has been instrumented in order to verify that the BIS-MDT chamber Module-0 fulfills its design requirements. The analysis of its data shows that the chamber meets these requirements; it has low noise levels, uniform drift properties, good spatial resolution, and high particle detection efficiency
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