Circular RNA hsa_circ_0000690 as a potential biomarker for diagnosis and prognosis of intracranial aneurysm: Closely relating to the volume of hemorrhage

Abstract Purpose This study aimed to explore circular RNA (circRNA) hsa_circ_0000690 as a potential biomarker for diagnosis and prognosis of intracranial aneurysm (IA) and its relationship with clinical factors and complications of IA. Material/methods 216 IA patients admitted to the neurosurgery department of our hospital from January 2019 to December 2020 were selected as the experimental group, and 186 healthy volunteers were selected as the control group. The expression of hsa_circ_0000690 in peripheral blood was detected by quantitative real‐time PCR, and its diagnostic value was assessed by receiver operating characteristic curve. Relationship between hsa_circ_0000690 and clinical factors of IA was assessed by chi‐square test. Nonparametric test was used in univariate analysis, and regression analysis was used in multivariate analysis. Multivariate Cox proportional hazards regression analysis was used to analyze the survival time. Results CircRNA hsa_circ_0000690 of IA patients was relatively lower than that in the control group (p < .001). The AUC of hsa_circ_0000690 was 0.752, the specificity was 0.780, and sensitivity was 0.620, with diagnostic threshold of 0.0449. In addition, hsa_circ_0000690 expression was correlated with Glasgow Coma Scale, the volume of subarachnoid hemorrhage, modified Fisher scale, Hunt–Hess levels and surgical type. For hydrocephalus and delayed cerebral ischemia, hsa_circ_0000690 was significant in univariate analysis, but nonsignificant in multivariate analysis. For prognosis, hsa_circ_0000690 was significantly associated with modified Rankin Scales after surgery for 3 months, but not associated with survival time. Conclusions The expression of hsa_circ_0000690 can act as a diagnostic marker for IA and predict the prognosis of 3 months after operation and is closely related to the volume of hemorrhage

Temperature-dependent evolution of hydroxyl radicals from peroxymonosulfate activation over nitrogen-modified carbon nanotubes

© 2018 Elsevier B.V. Rational regulation of redox capacity in advanced oxidation processes (AOPs) by metal-free nanomaterials is appealing to extend the state-of-the-art carbocatalysis toward diverse applications. In this study, nitrogen-decorated single-walled carbon nanotubes (N-SWCNT) were fabricated using urea as a green precursor under thermal pyrolysis. We comprehensively investigated the peroxymonosulfate (PMS) activation at varying temperatures with/without the nanocarbon toward nitrobenzene (NB) oxidation. In the noncatalytic system, NB decomposition rate was impressively boosted with the raising reaction temperatures. More importantly, hydroxyl radicals (•OH) were unveiled to be the primary reactive oxygen species (ROS) and contributed to NB degradation under high temperatures in PMS/N-SWCNT system. The mechanism of PMS activation with/without N-SWCNT and impact of temperature were elucidated by both in situ electron paramagnetic resonance (EPR) technique and selectively radical quenching tests. Interestingly, the NB degradation kinetics witnessed a two-stage process with different activation energies in PMS only and PMS/N-SWCNT systems, suggesting that the reaction temperature may be able to tune the catalytic mechanism and generated ROS. Therefore, the nanocarbon-catalyzed and thermal-assisted metal-free AOPs can be featured with maneuverable oxidative potentials toward a wide range of redox processes