Metabolomic and high-throughput sequencing analysis—modern approach for the assessment of biodeterioration of materials from historic buildings

Preservation of cultural heritage is of paramount importance worldwide. Microbial colonization of construction materials, such as wood, brick, mortar and stone in historic buildings can lead to severe deterioration. The aim of the present study was to give modern insight into the phylogenetic diversity and activated metabolic pathways of microbial communities colonized historic objects located in the former Auschwitz II-Birkenau concentration and extermination camp in Oświęcim, Poland. For this purpose we combined molecular, microscopic and chemical methods. Selected specimens were examined using Field Emission Scanning Electron Microscopy (FESEM), metabolomic analysis and high-throughput Illumina sequencing. FESEM imaging revealed the presence of complex microbial communities comprising diatoms, fungi and bacteria, mainly cyanobacteria and actinobacteria, on sample surfaces. Microbial diversity of brick specimens appeared higher than that of the wood and was dominated by algae and cyanobacteria, while wood was mainly colonized by fungi. DNA sequences documented the presence of 15 bacterial phyla representing 99 genera including Halomonas, Halorhodospira, Salinisphaera, Salinibacterium, Rubrobacter, Streptomyces, Arthrobacter and 9 fungal classes represented by 113 genera including Cladosporium, Acremonium, Alternaria, Engyodontium, Penicillium, Rhizopus and Aureobasidium. Most of the identified sequences were characteristic of organisms implicated in deterioration of wood and brick. Metabolomic data indicated the activation of numerous metabolic pathways, including those regulating the production of primary and secondary metabolites, for example, metabolites associated with the production of antibiotics, organic acids and deterioration of organic compounds. The study demonstrated that a combination of electron microscopy imaging with metabolomic and genomic techniques allows to link the phylogenetic information and metabolic profiles of microbial communities and to shed new light on biodeterioration processes

Monitoring of trace metals, biochemical composition and growth of Axillary seabream (Pagellus acarne Risso, 1827) in offshore Copper alloy net cage

The study was conducted to assess trace metal contents, biochemical composition and growth performance of axillary seabream (Pagellus acarne Risso, 1827) cultured in a copper alloy mesh cage. A total of 400 axillary seabream (initial mean weight: 176.0±14.0 g), a new candidate species for the Mediterranean aquaculture, were stocked into a high-density polyethylene frame gravity cage and fed a commercial seabream diet for a period of 6 months. At the end of the feeding trial, fish reached a final weight of 264.8±16.8 g with a weight increase of 88.8 g and a feed conversion rate of 2.51. Overall, relative growth rate, specific growth rate and feed conversion ratio were satisfactory and comparable to the pelagic fishes such as gilthead seabream or European seabass, which are presently the main fish species for the Mediterranean aquaculture industry. Trace elements in fish grown in copper alloy net cages over a 6-month period showed satisfactory results, as the metal concentrations in fish tissues such as liver, skin, muscle and gills were below the reported upper limits for human consumption, indicating that copper alloy net is an acceptable and safe material for finfish cage aquaculture. Furthermore, from the growth performance data obtained in the present study, it can be concluded that axillary seabream showed potential for cage farming, and thus is a promising new candidate for the Mediterranean aquaculture industry

Cultural variations in the relationship between anger coping styles, depression and life satisfaction

Hypotheses are tested that ways of handling anger and their consequences will differ in student samples drawn from dignity cultures (UK and Finland), honor cultures (Turkey and Pakistan) and face cultures (Hong Kong and China). In line with our hypotheses, holding anger in and controlling anger correlate positively in face cultures but not in other samples, whereas holding anger in and letting anger out correlate positively in honor cultures but not in other samples. Furthermore, holding anger in and letting anger out are more strongly predictive of high depression and low life satisfaction in honor cultures than in other samples. The results provide support for the cross-cultural validity of Spielberger's (1999) anger expression inventory and for the proposition that differences in ways of handling anger can be understood in terms of contrasting cultural contexts

Nation-level moderators of the extent to which self-efficacy and relationship harmony predict students’ depression and life satisfaction: evidence from ten cultures

Previous two-nation comparisons have provided evidence that self-efficacy may be a protective factor against depression in individualist cultures, whereas relationship harmony may be a stronger protective factor in collectivist cultures. However, wider sampling and more specific measures of cultural difference are required to test these conclusions. Student ratings of depression and life satisfaction were surveyed in 10 samples drawn from nine nations. Culture-level individualism positively moderated the relationship of self-efficacy to low depression. However, culture-level collectivism negatively moderated the linkage of relationship harmony to depression. To better understand these effects, four separate nation-level predictors derived from dimensions of self-construal were employed. Effects of self-efficacy were strongest where cultural models of selfhood emphasized self-direction (vs. receptiveness to influence); effects of relationship harmony were strongest where cultural models of selfhood emphasized dependence on others (vs. self-reliance). These results illustrate the value of unpackaging the diffusely defined concept of individualism-collectivism

Secondary hyperfunction of the contralateral inferior oblique muscle after the unilateral inferior oblique weakening procedure.

Purpose This study was designed to analyze the consecutive overaction of the contralateral inferior oblique (IO) muscle after unilateral weake in procedure of the same muscle which is a well-known but not regularly studied phenomenon

Pollution Trading in Water Quality Limited Areas: Use of Benefits Assessment and Cost-Effective Trading Ratios

This paper proposes a water quality trading design that addresses common implementation problems. Trading ratios, which are calculated from damages integrated over each source’s spatial zone of influence, drive the system to a socially costeffective outcome. The design is applied to combined sewer overflow management in the Upper OhioRiver Basin, where trading ratios can vary significantly among trading partners. The analysis shows that significant compliance cost savings are possible without incurring a penalty in terms of social damages or overall water quality despite a higher level of discharge relative to the command and control option.