568 research outputs found
Studying Light-Harvesting Models with Superconducting Circuits
The process of photosynthesis, the main source of energy in the animate
world, converts sunlight into chemical energy. The surprisingly high efficiency
of this process is believed to be enabled by an intricate interplay between the
quantum nature of molecular structures in photosynthetic complexes and their
interaction with the environment. Investigating these effects in biological
samples is challenging due to their complex and disordered structure. Here we
experimentally demonstrate a new approach for studying photosynthetic models
based on superconducting quantum circuits. In particular, we demonstrate the
unprecedented versatility and control of our method in an engineered three-site
model of a pigment protein complex with realistic parameters scaled down in
energy by a factor of . With this system we show that the excitation
transport between quantum coherent sites disordered in energy can be enabled
through the interaction with environmental noise. We also show that the
efficiency of the process is maximized for structured noise resembling
intramolecular phononic environments found in photosynthetic complexes.Comment: 8+12 pages, 4+12 figure
Alteration of glyoxalase genes expression in response to testosterone in LNCaP and PC3 human prostate cancer cells.
Glyoxalase system, a ubiquitous detoxification pathway protecting against cellular damage caused by potent cytotoxic metabolites, is involved in the regulation of cellular growth. Aberrations in the expression of glyoxalase genes in several human cancers have been reported. Recently, we described a possible regulatory effect by estrogens on glyoxalase genes in human breast cancer cell lines. This result, along with those ones regarding changes in glyoxalases activity and expression in other human hormone-regulated cancers, such as prostate cancer, has prompted us to investigate whether also androgens, whose functional role in prostate cancer pathogenesis is well known, could modulate glyoxalases gene expression. Therefore, we treated LNCaP androgen-responsive and PC3 androgen-independent human prostate cancer cell lines with testosterone at the concentrations of 1 nM and 100 nM. After a two days treatment, glyoxalases mRNA levels as well as cell proliferation were evaluated by real-time RT-PCR analysis and [3H]thymidine incorporation, respectively. Results pointed out that testosterone affects the expression of glyoxalase system genes and cell proliferation in a different manner in the two cell lines. The possibility that modulation of glyoxalase genes expression by testosterone is due to glyoxalases-mediated intracellular response mechanisms to the androgen-induced oxidative stress or to the presence of androgen response elements (ARE) in glyoxalase promoters are discussed. Knowledge regarding the regulation of glyoxalases by testosterone may provide insights into the importance of these enzymes in human prostate carcinomas in vivo
Resistance to clarithromycin and genotypes in Helicobacter pylori strains isolated in Sicily
The resistance of Helicobacter pylori strains to clarithromycin is increasing in several developed countries and their association with a genetic pattern circulation has been variously explained as related to different geographical areas. In this study we have reported: the prevalence of the resistance of H. pylori, isolated in Sicily, to clarithromycin; the principal point of mutation associated with this resistance; and the more frequent association between resistance to clarithromycin and cagA, the EPIYA motif, and the vacA and oipA genes. Resistance to clarithromycin was detected in 25 % of cases, the main genetic mutation involved being A2143G. The cagA gene was present in 48 % of cases and the distribution of the EPIYA motif was: ABC in 35 cases; ABCC in 8 cases; ABCCC in 2 cases; ABC-ABCC in 2 cases; and ABC-ABCC-ABCCC in 1 case. Regarding the vacA allele, an s1i1m1 combination was detected in 35 % of cases, s1i1m2 in 12 %, s1i2m2 in 12 %, s2i2m2 in 40 %, and a double s1m1-m2 mosaic in 1 % of cases. The status of the oipA gene was âoffâ in 45 % of cases and âonâ in 55 %. Resistance to clarithromycin was found to be high in Sicily, but no correlation was found among resistance to clarithromycin, the vacA gene and oipA status; a higher correlation was observed between resistant strains and cagA-negative strains
- âŠ