Reduction in Chaetoceros populations by furanace

One of the most promising prophylactic agents being tested to control Penaeus monodon larval diseases is furanace (6-hydroxymethyl-2 2(5-nitro-2-furyl) vinyl pyridine). To evaluate further its suitability as a chemotherapeutic agent, its effects on the population growth of Chaetoceros calcitrans, the diatom used as feed for the zoeal stages, was examined. Chaetoceros populations of uniform density (initial density in all runs: 130-141x10 SUP-3 cells /ml) were placed in nine white, circular (382 sq cm), plastic basins. The physio-chemical characteristics of the culture water were as follows: salinity, 28 . 5-30 . 0 ppt; pH, 8 . 62-8 . 72; temperature, 23-25 . 5 degree C; dissolved oxygen, 7 . 1-9 . 3 ppm; nitrate, 0 . 03-0 . 07 ppm; and ammonia, 0 . 005-0 . 03 ppm. Preweighed furanace granules were dissolved in the culture water, with resulting concentrations of 1 and 2 mg/l (3 replicates each). A set of replicates without furanace served as the control. Population counts of the diatom were taken after 2, 4, 6, 8, and 10 hr exposures. After 4 hr, the population decreased in all three levels. The population in 2 mg/l furanace showed the lowest count and that in control the highest. The population means are not statistically different from one another. The results of the study show that the furanace causes reductions in Chaetoceros population at all durations of exposure

Effects of furanace on Brachionus

Tiger prawn P.monodon) larvae utilize Brachionus a rotifer, as food in the Zoea 3 and mysis stages when they change from an herbivorous to an omnivorous diet. The present work aims to show the effects of furanace on the population growth of Brachionus. Cultures of Brachionus were obtained and fed with Chlorella at a density of 1-2x10 SUP-6 cells/ml. Five liters of the culture water were placed in each of 4 white, circular, 152x304 mm plastic basins. The mean initial densities of the rotifer ranged from 26 . 5 to 38 . 5 individuals/ml. The concentrations of furanace were 0, 1, 2 and 3 mg /l. The cultures were vigorously aerated. Population growth was observed after 3, 6, and 9 hours of exposure. The cultures were thoroughly mixed before samples were taken to ensure an almost equal distribution of the rotifers in the water. To facilitate the counting of the rotifer, one drop of Lugol's solution was added to each sample. This immobilizes the rotifer as well as stops further reproduction. Individuals with only the lorica left or with badly deformed lorica were considered dead. Population counts were done using a Sedgwick-Rafter counting chamber. Among the different durations of exposure, the percentage survival of the populations in the furanace baths were highest after 3 hr. There were slight increases in the control and 2 mg/l and slight decreases in 1 and 3 mg/l. The differences in the mean densities are statistically insignificant at . 01 significance level. After a 6-hr exposure, the control population reached its peak density with a survival of 89%. Populations in furanace baths decreased to 88 . 5% in both 2 and 3 mg /l followed closely by 87% in 1 mg/l. Again, no statistical differences exist among all the levels. The mean percentage survival in 1 and 2 mg/l increased (89% and 91%, respectively) after a 9-hr expsoure, while those in the control and 3 mg/l decreased to 86 . 5% and 88 . 25%, respectively. There were no marked differences in appearance noted among the individuals in furanace baths and those in the control

Isolation and culture in artificial media of Lagenidium from Penaeus monodon larvae

Fungal infection of P. monodon larvae is a problem in hatchery operations. The fungus, which attacks the nauplius to postlarval stages and causes up to 100% mortality, has been tentatively identified as belonging to the genus Lagenidium . This pathogenic organism has recently been isolated and cultured. A description is given of the fungus, and features of its biology and pathology are discussed

The Impact of Spatial Form of Urban Architecture on the Urban Thermal Environment: A Case Study of the Zhongshan District, Dalian

The correlation of spatial variation in land surface temperature with building height and density in Zhongshan District, Dalian, China, is investigated over the period 2003-2013. We utilize remote sensing data and multisource land-use data, which we evaluate using a combination of a single-window algorithm and correlation analysis. The results show that: 1) during 2003-2013, the number of high surface temperature regions increased by 4339 grid cells (out of a total of 53 601), with most of the high-temperature grid cells distributed along Jiefang Road and Zhongnan Road in the northern part of Zhongshan District. Ninety-eight percent of grid cells had temperature ranges of 293-309 K in July 2003, 296-310 K in August 2003, 295-308 K in July 2008, 296-311 K in August 2008, 305-314 K in July 2013, and 303-318 K in August 2013; 2) during 2003-2008, the number of low-rise buildings increased by 140%, and the number of multistory buildings increased by 100%. During 2008-2013, the number of buildings in all height categories remained relatively unchanged, with the exception of an 11% decrease in the number of low-rise buildings; 3) surface temperature showed weak correlation with building height (0.314, 0.346, and 0.361 in 2003, 2008, and 2013, respectively) but moderate correlation with building density (0.511, 0.533, and 0.563, respectively)

Population biology and secondary production of the stout razor clam Tagelus plebeius (Bivalvia, Solecurtidae) on a sandflat in southeastern Brazil

The population biology and production of the stout razor clam Tagelus plebeius Lightfoot, 1786 were investigated on an intertidal sandflat on the southeast coast of Brazil (Enseada Beach, São Sebastião, state of São Paulo) between April 1997 and April 1998. Two rectangular sites of 50 X 10 m parallel to the waterline were established, site A (upper intertidal level) and site B (middle intertidal level), where the samples were taken in an 0.5 x 0.5 m quadrat. High abundances were recorded in winter and spring, with no significant differences between the sites. The high bivalve abundances were related to the presence of very fine homogeneous sediment with low salinities. Tagelus plebeius had negative allometric growth, characteristic of deep burrowers for the relationships DM/SL and AFDM/SL. Parameters of the modified von Bertalanffy growth function were: L∞ = 67.01 mm, K = 1.73 year-1, t0 = -0.11 year, C = 0.43, WP = 0.96. The instantaneous mortality (Z) was 3.12 year-1, relatively high in comparison to other tropical bivalve populations. Secondary production was 1.53 g AFDM m-2 year-1, with a P/B ratio reaching 1.37 year-1. This high turnover ratio (P/B) was related to a rapid population replacement, connected with the short life span and high mortality of the species.546

Training proposal for community health agents in hearing health

TEMA: capacitação de agentes comunitários de saúde na área de saúde auditiva. OBJETIVO: verificar a efetividade de um programa de capacitação de agentes comunitários de saúde do Programa de Saúde da Família, na área de saúde auditiva infantil. MÉTODO: a casuística constou de dois grupos: grupo A foi constituído por 31 agentes comunitários de saúde da cidade de Bauru e, grupo B, formado por 75 agentes comunitários de saúde de Sorocaba, ambos municípios do Estado de São Paulo. A capacitação foi realizada por meio de aulas expositivas para os dois grupos, contudo para o grupo A foi utilizado uma apostila adaptada da World Health Organization (2006), para que os agentes comunitários de saúde pudessem acompanhar as atividades realizadas de forma interativa. A capacitação abordou os temas: audição e deficiência auditiva, tipos, prevenção e causas da deficiência auditiva, técnicas de identificação e diagnóstico da deficiência auditiva e aspectos gerais da deficiência auditiva. Para validar a capacitação foi aplicado um questionário pré e pós-capacitação com perguntas sobre os assuntos que foram abordados no decorrer do curso, a fim de analisar a assimilação do conteúdo ministrado. RESULTADOS: a capacitação foi efetiva, com aumento no escore total obtido nos questionários pré e pós-capacitação. CONCLUSÕES: os resultados comprovam a eficácia do programa de capacitação com utilização de material e abordagem interativa proposto para os agentes comunitários de saúde dos Programas de Saúde da Família.BACKGROUND: training of community health agents in hearing health. AIM: to verify the effectiveness of a training program regarding the hearing health of children for Community Health Agents of a Family Health Program. METHOD: research sample consisted of two groups: Group A, constituted by 31 community health agents, from Bauru, SP, and Group B, constituted by 75 community health agents, from Sorocaba, SP. Training was provided through classes for both groups. For Group A, a text adapted from the material organized by the World Health Organization (2006) was used so that the community health agents could follow the activities in an interactive way. Training focused on the following topics: normal and impaired hearing; types, prevention and causes of hearing loss; procedures to identify and diagnose the hearing impairment and rehabilitation. Pre and post training questionnaires were used to assess the assimilation of the presented content. RESULTS: training demonstrated to be effective, since there was an increase in the overall score when comparing the scores obtained in the pre and post training questionnaires CONCLUSION: the results indicate the effectiveness of the training program for community health agents of a Family Health Program with the use of texts and with an interactive approach.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Complement Expression in the Retina is not Influenced by Short-term Pressure Elevation

Purpose: To determine whether short-term pressure elevation affects complement gene expression in the retina in vitro and in vivo. Methods: Muller cell (TR-MUL5) cultures and organotypic retinal cultures from adult mice and monkeys were sub- jected to either 24-h or 72-h of pressure at 0, 15, 30, and 45 mmHg above ambient. C57BL/6 mice were subjected to microbead-induced intraocular pressure (IOP) elevation for 7 days. RNA and protein were extracted and used for analysis of expression levels of complement component genes and complement component 1, q subcomponent (C1q) and comple- ment factor H (CFH) immunoblotting. Results: mRNA levels of complement genes and C1q protein levels in Muller cell cultures remained the same for all pressure levels after exposure for either 24 or 72 h. In primate and murine organotypic cultures, pressure elevation did not produce changes in complement gene expression or C1q and CFH protein levels at either the 24-h or 72-h time points. Pressure-related glial fibrillary acidic protein (GFAP) mRNA expression changes were detected in primate retinal organotypic cultures (analysis of variance [ANOVA]; p0.05 for both) with contralateral control and naïve control eyes. Conclusions: Short-term elevation of pressure in vitro as well as short-term (1 week) IOP elevation in vivo does not seem to dramatically alter complement system gene expression in the retina. Prolonged expression to elevated pressure may be necessary to affect the complement system expression