Effects of water stress on spectral reflectance of bermudagrass

In the south-central Italy, during summer rainfall does not supply a sufficient amount of water. Therefore, irrigation management during dry periods is important for maintaining turf quality. The hybrid bermudagrass (Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt–Davy) is known to represent the dominant warm-season turfgrass in warm to temperate climatic regions and its drought tolerance make bermudagrass a competitive turfgrass. A greenhouse experiment was conducted using uniform cores of hybrid bermudagrass, which were secured in a polyvinyl chloride cylinders and watered by constant sub-irrigation. The objectives of the present research were to measure the spectral reflectance with a new generation handheld spectroradiometer on hybrid bermudagrass and to explore various vegetation indices to be used as future detecting tool to study water stress in bermudagrass. Moreover, the potential uses of multivariate processing techniques for discriminating different water stress conditions in turfgrass has been investigated. Besides spectral indices, multivariate methods, although performed on a data set limited in terms of sample size, have shown a great potential for water stress monitoring in turfgrass and surely deserve further investigations. There are different indices that use distinct water absorption features independent of chlorophyll concentration, such as water index (WI = R900/R970) that has been reported to be a robust index of canopy water content and is used as an active indicator of changes in Leaf Relative Water Content (LRWC). Also, the ratio of WI with NDVI (WI/NDVI = (R900/R970)/((R800 − R680)/(R800 + R680)]) was found to be an effective indicator of water stress. Another vegetation index to detect water features is normalized difference water index (NDWI), designed to maximize reflectance of water by using green wavelengths. In our trial in bermudagrass the relationships studied, suggest that WI (900/970) and WI/NDVI, among the indices studied, are the more effective indicators of water stress. In fact, lower values of WI indicate higher water stress, while higher values of WI/NDVI indicate higher water stress levels

Robotic mowing of tall fescue at 90 mm cutting height: random trajectories vs. systematic trajectories

Tall fescue (Schedonorus arundinaceus (Schreb.) Dumort.) is often managed with a cutting height ranging from 70 to 100 mm in ornamental lawns. Some autonomous mowers have been specifically designed to maintain mowing height in the same range. Generally, autonomous mowers operate by following random trajectories, and substantial overlapping is needed to obtain full coverage of the working area. In the case of tall grass, this may cause lodging of grass plants, which in turn may reduce turf quality. The introduction of a navigation system based on systematic trajectories has the potential to improve the performances of autonomous mowers with respect to machine efficiency and turf quality. With the aim of determining the effects of reduced mowing frequency and systematic navigation systems on turf quality and mower performances in terms of working time, energy consumption and overlapping, the performances of two autonomous mowers working with random and systematic trajectories were tested on a mature tall fescue lawn at 90 mm cutting height. The working efficiency was approximately 80% for the systematic trajectories and approximately 35% for the random trajectories; this was mainly due to the lower overlapping associated with systematic trajectories. Turf quality was slightly higher for the mower working systematically (a score of 8 using a 1–9 score with 1 = poor, 6 = acceptable and 9 = best) compared to the one working randomly (quality of 7 and 6 on a 1–9 scale with 1 = poor and 9 = best). No appreciable lodging was observed in either case. For tall, managed lawns, systematic trajectories may improve autonomous mowers’ overall performances

The Immune Landscape of Papillary Thyroid Cancer in the Context of Autoimmune Thyroiditis

Simple Summary The association between papillary thyroid cancer and Hashimoto's thyroiditis went through a long-standing human debate recently elucidated by the establishment of a novel mouse model. Papillary thyroid carcinoma is an excellent model for studying the tumor immune microenvironment because it is naturally accompanied by immune cells, making it a good candidate for the treatment with immune checkpoint inhibitors. Papillary thyroid cancer (PTC) often co-occurs with Hashimoto's thyroiditis, an association that has long been reported in clinical studies, remaining controversial. Experimental evidence has recently shown that pre-existing thyroiditis has a beneficial effect on PTC growth and progression by a distinctive expansion of effector memory CD8 T cells. Although the link between inflammation and PTC might involve different components of the immune system, a deep characterization of them which includes T cells, B cells and tertiary lymphoid structures, Mye-loid cells, Neutrophils, NK cells and dendritic cells will be desirable. The present review article considers the role of the adaptive and innate immune response surrounding PTC in the context of Hashimoto's thyroiditis. This review will focus on the current knowledge by in vivo and in vitro studies specifically performed on animals' models; thyroid cancer cells and human samples including (i) the dual role of tumor-infiltrating lymphocytes; (ii) the emerging role of B cells and tertiary lymphoid structures; (iii) the role of myeloid cells, dendritic cells, and natural killer cells; (iv) the current knowledge of the molecular biomarkers implicated in the complex link between thyroiditis and PTC and the potential implication of cancer immunotherapy in PTC patients in the context of thyroiditis

Autonomous mower vs. rotary mower: effects on turf quality and weed control in tall fescue lawn

Autonomous mowers are battery-powered machines designed for lawn mowing that require very low human labour. Autonomous mowers can increase turf quality and reduce local noise and pollution compared with gasoline-powered rotary mowers. However, very little is known about the effects of autonomous mowing on encroaching weeds. The aim of this research was to compare the effects of an autonomous mower and an ordinary gasoline-powered mower on weed development in an artificially infested tall fescue (Festuca arundinacea Schreb.) turf with different nitrogen (N) rates. A three-way factor experimental design with three replications was adopted. Factor A consisted of three N rates (0, 75, and 150 kg ha1), factor B consisted of two mowing systems (autonomous mower vs. walk-behind gasoline rotary mower equipped for mulching), and factor C which consisted of four different transplanted weed species: (a) Bellis perennis L., (b) Trifolium repens L.; (c) Trifolium subterraneum L.; and (d) Lotus corniculatus L. Of these, B. perennis is a rosette-type plant, while the other three species are creeping-type plants. The interaction between mowing system and transplanted weed species showed that the four transplanted weed species were larger when mowed by the autonomous mower than by the rotary mower. The autonomous mower yielded larger weeds probably because the constant mowing height caused the creeping weed species to grow sideways, since the turfgrass offered no competition for light. N fertilization increased turf quality and mowing quality, and also reduced spontaneous weed infestation. Autonomous mowing increased turf quality, mowing quality, but also the percentage of spontaneous weed cover

Trampling Analysis of Autonomous Mowers: Implications on Garden Designs

Several trials have been carried out by various authors concerning autonomous mowers, which are battery-powered machines. The effects of these machines on turfgrass quality and energy consumption have been thoroughly investigated. However, there are still some aspects that have not been studied. Among these, random trajectory overlapping is one of the most important. To investigate these aspects, two RTK-GPS devices along with the custom-built software used for previous trials has been upgraded in order to precisely calculate how many times the mower drives over the same spot using random trajectories. This parameter, the number of passages in the same position, was hypothesized to explain the autonomous mower's overlapping and trampling action. The trial has been carried out testing a commercial autonomous mower on three areas with different levels of complexity to assess its performances. The following variables were examined: the percentage of mowed area, the distance travelled, the number of intersections, the number of passages, and the autonomous mower's work efficiency. The average percentage of area mown (average value for the three areas) was 54.64% after one hour and 80.15% after two hours of work. Percentage of area mown was 15% higher for the area with no obstacles after two hours of work. The number of passages was slightly different among the three garden designs. The garden with no obstacles obtained the highest number of passages with an average of 37 passages. The highest working efficiency was obtained in the garden with an intermediate number of obstacles with a value of 0.40 after two hours of work. The estimated energy consumption resulted 0.31 Wh m(-2) after one hour and 0.42 Wh m(-2) after two hours of working. These results highlight how the correct settings of cutting time may be crucial to consistently save energy during the long period and may be useful for a complete automation of the maintenance of green areas

Assessment of the cutting performance of a robot mower using custom built software.

Before the introduction of positioning technologies in agriculture practices such as global navigation satellite systems (GNSS), data collection and management were time-consuming and labor-intensive tasks. Today, due to the introduction of advanced technologies, precise information on the performance of agricultural machines, and smaller autonomous vehicles such as robot mowers, can be collected in a relatively short time. The aim of this work was to track the performance of a robot mower in various turfgrass areas of an equal number of square meters but with four dierent shapes by using real-time kinematic (RTK)-GNSS devices, and to easily extract data by a custom built software capable of calculating the distance travelled by the robot mower, the forward speed, the cutting area, and the number of intersections of the trajectories. These data were then analyzed in order to provide useful functioning information for manufacturers, entrepreneurs, and practitioners. The path planning of the robot mower was random and the turfgrass area for each of the four shapes was 135 m2 without obstacles. The distance travelled by the robot mower, the mean forward speed, and the intersections of the trajectories were aected by the interaction between the time of cutting and the shape of the turfgrass. For all the dierent shapes, the whole turfgrass area was completely cut after two hours of mowing. The cutting eciency decreased by increasing the time, as a consequence of the increase in overlaps. After 75 minutes of cutting, the eciency was about 35% in all the turfgrass areas shapes, thus indicating a high level of overlapping

Autonomous Mowing and Complete Floor Cover for Weed Control in Vineyards

Enhancing vineyards sustainability and reducing herbicides usage is a crucial theme, thus alternative weed management methods are starting to be studied. Cover crops have been shown to provide for several environmental services such as performing an efficient weed control and promoting biodiversity, thus improving the sustainability of the overall management system. However, the use of cover crops is usually confined to the interrow area in order to avoid competition with vines. Under-trellis weed management in vineyards is an important challenge, conventionally fulfilled by cultivation or repeated herbicides applications. Autonomous mowers are small autonomous machines that have shown a great efficiency when employed in agricultural contexts. Due to their reduced size, they can easily prevent the excessive vertical growth of weeds both in the interrows and under trellis without the use of chemical applications. The aim of this trial was to evaluate if the combination of cover crop species and autonomous mowers management could improve vineyard sustainability. Vineyard floor cover that was managed with an autonomous mower had a shorter canopy height and a lower weed dry biomass compared to the conventionally managed vineyard floor cover. Vineyard floor management providing cover crops and autonomous mowing had a significantly lower weed cover percentage compared to conventional floor management, especially under-trellis. The results of this trial suggest that the combination of cover crops and autonomous mowers may be a sustainable and reliable technique to include in vineyards floor management

Comparison between different rotary mowing systems: Testing a new method to calculate turfgrass mowing quality

Poor quality in turfgrass mowing is highlighted by the shredded leaf tips with necrotic tissues that give an unsightly brownish colour to the turf and may also lead to turf disease. Mowing quality is also typically assessed by visual rating, thus the score depends on the person doing the assessment. To make the evaluation of mowing quality not subjective, an innovative method was developed. The aim of the trial was to examine the effects of different mowing systems and two different nitrogen rates (100 and 200 kg ha−1 ) on two turfgrass species in order to test the new mowing quality calculation. Three different mowing systems were used: a battery-powered rotary mower set at 3000 rpm and 5000 rpm respectively and a gasoline-powered rotary mower set at full throttle. The battery-powered mower at low blade rpm produced a poorer mowing quality and turf quality than the gasoline-powered mower and battery-powered mower at high rpm, which produced a similar mowing quality and turf quality. Leaf tip damage level values showed a significant correlation with the results of the visual mowing quality assessment. Lower leaf tip damage level values (slightly above 1) corresponded to higher visual mowing quality scores (around 8)

Pituitary and systemic autoimmunity in a case of intrasellar germinoma

Germinomas arising in the sella turcica are difficult to differentiate from autoimmune hypophysitis because of similar clinical and pathological features. This differentiation, nevertheless, is critical for patient care due to different treatments of the two diseases. We report the case of an 11-year-old girl who presented with diabetes insipidus and growth retardation, and was found to have an intra- and supra-sellar mass. Initial examination of the pituitary biopsy showed diffuse lymphocytic infiltration of the adenohypophysis and absent placental alkaline phosphatase expression, leading to a diagnosis of hypophysitis and glucocorticoid treatment. Because of the lack of clinical and radiological response, the pituitary specimen was re-examined, revealing this time the presence of scattered c-kit and Oct4 positive germinoma cells. The revised diagnosis prompted the initiation of radiotherapy, which induced disappearance of the pituitary mass. Immunological studies showed that the patient’s serum recognized antigens expressed by the patient’s own germinoma cells, as well as pituitary antigens like growth hormone and systemic antigens like the Sjögren syndrome antigen B and alpha-enolase. The study first reports the presence of pituitary and systemic antibodies in a patient with intrasellar germinoma, and reminds us that diffuse lymphocytic infiltration of the pituitary gland and pituitary antibodies does not always indicate a diagnosis of autoimmune hypophysitis

St. Augustinegrass accessions planted in northern, central and southern Italy: Growth and morphological traits during establishment

The use of warm season turfgrasses is a consolidated trend in the climatic transition zone of Mediterranean countries, in particular St. Augustinegrass (Stenotaphrum secundatum (Walt.) Kuntze) begins to be widespread in warm coastal areas. However, little is known about the performance of the different cultivars of this species in southern Europe. In 2016-2017 a trial was carried out in three locations in Italy, Padova, Pisa. and Palermo, located in the north, center and south of the country respectively. Four cultivars (Floratine, Captiva, Sapphire, Pahnetto) and five ecotypes (CeRTES 201, CeRTES 202, CeRTES 203, CeRTES 204, CeRTES 205) were compared in terms of their growth characteristics and morphological traits during establishment. The results highlighted that stolon growth was significantly affected by the location, as well as green colour retention. Stolon growth rate, internode length and internode volume and turf quality were, however, significantly determined by the accession effect. The quality of the ecotypes was also in some cases comparable to that of the cultivars. In Padova, winterkill occurred in most of the accessions, while in Pisa and Palermo, all the entries survived. In conclusion. St. Augustinegrass is suitable for turf use in the central and southern coastal area of Italy