Fator Cronológico na Credibilidade do Evolucionismo entre os Discentes da Universidade Severino Sombra

Os conceitos evolucionistas começaram a se fundamentar a partir de Descartes, Buffon, Lamarck e Darwin e ao mesmo tempo despertaram as contradições das doutrinas criacionistas. A credibilidade do evolucionismo por faixa etária dos alunos de graduação da Universidade Severino Sombra (USS), nas diferentes áreas do saber foi o objeto deste trabalho. A população discente se concentrou na faixa etária entre 20 a 24 anos em que predomina o acreditar em ideias evolucionistas, não se podendo avaliar se estes posicionamentos independem da divulgação ou do ensino do criacionismo introduzido há algum tempo nas escolas públicas de alguns estados brasileiros. Pode-se inferir, a partir deste estudo, um grande desconhecimento sobre a teoria científica evolucionista entre os discentes dos cursos superiores pesquisados na USS

Preliminary Analysis of Sm14 in Distinct Fractions of Schistosoma mansoni Adult Worm Extract

In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni   infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica   in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE, and is being developed as the molecular basis of an anti-helminth bivalent vaccine against the two parasites, for medical and veterinary application. Present data refer to SDS-PAGE and Western Blotting analysis of four different preparations of S. mansoni   adult worms focusing Sm14 identification. The extracts correspond to the initial fraction of the SE extraction process, containing products released by living worms (SEi); SE2, reextraction of adult worms in PBS; and SE of separated male and female adult worms. In all extracts it was possible to detect the component of 14 kDa, that was recognized by specific anti-rSm14 antibody raised in rabbits

Mouse Tracking Measures and Movement Patterns with Application for Online Surveys

Part 1: MAKE-Main TrackInternational audienceThere is growing interest in the field of human-computer interaction in the use of mouse movement data to infer e.g. user’s interests, preferences and personality. Previous work has defined various patterns of mouse movement behavior. However, there is a paucity of mouse tracking measures and defined movement patterns for use in the specific context of data collection with online surveys. The present study aimed to define and visualize patterns of mouse movements while the user provided responses in a survey (with questions to be answered using a 5-point Likert response scale). The study produced a wide range of different patterns, including new patterns, and showed that these can easily be distinguished. The identified patterns may - in conjunction with machine learning algorithms - be used for further investigation toward e.g. the recognition of the user’s state of mind or for user studies

Fator Cronológico na Credibilidade do Evolucionismo entre os Discentes da Universidade Severino Sombra

Os conceitos evolucionistas começaram a se fundamentar a partir de Descartes, Buffon, Lamarck e Darwin e ao mesmo tempo despertaram as contradições das doutrinas criacionistas. A credibilidade do evolucionismo por faixa etária dos alunos de graduação da Universidade Severino Sombra (USS), nas diferentes áreas do saber foi o objeto deste trabalho. A população discente se concentrou na faixa etária entre 20 a 24 anos em que predomina o acreditar em ideias evolucionistas, não se podendo avaliar se estes posicionamentos independem da divulgação ou do ensino do criacionismo introduzido há algum tempo nas escolas públicas de alguns estados brasileiros. Pode-se inferir, a partir deste estudo, um grande desconhecimento sobre a teoria científica evolucionista entre os discentes dos cursos superiores pesquisados na USS

Genomic characterisation of a novel KPC-98-producing clinical Klebsiella pneumoniae strain conferring resistance to ceftazidime/avibactam

© 2023 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.Ceftazidime/avibactam (CZA) is a novel drug that inactivates Ambler class A, including Klebsiella pneumoniae carbapenemases (KPC), class C and class D β-lactamases, but is not active against metallo-β-lactamases (class B). Despite its limited use worldwide, emerging cases of CZA-resistant isolates producing variants of the blaKPC gene have been reported. In this study, we report a novel KPC-98 variant conferring CZA resistance identified in a highly virulent and multidrug-resistant clinical isolate of K. pneumoniae, belonging to the high-risk international clone sequence type 13 (ST13).This research was partially funded by the Fundação para a Ciência e a Tecnologia (FCT) [grant nos. UIDB/04295/2020 and UIDP/04295/2020]. G.M. is supported by the Fundação para a Ciência e Tecnologia (FCT), Portugal, through a PhD Research Studentship Contract [Contrato de Bolsa de Investigação para Doutoramento 2020.07736.BD].info:eu-repo/semantics/publishedVersio

r-Sm14 - pRSETA efficacy in experimental animals

Previous studies carried out with Sm14 in experimental vaccination against Schistosoma mansoni   or Fasciola hepatica   infections were performed with recombinant Sm14 (rSm14) produced in Escherichia coli   by the pGEMEX system (Promega). The rSm14 was expressed as a 40 kDa fusion protein with the major bacteriophage T7 capsid protein. Vaccination experiments with this rSm14 in animal models resulted in consistent high protective activity against S. mansoni cercariae challenge and enabled rSm14 to be included among the vaccine antigens endorsed by the World Health Organization for phase I/II clinical trials. Since the preparation of pGEMEX based rSm14 is time consuming and results in low yield for large scale production, we have tested other E. coli expression systems which would be more suitable for scale up and downstream processing. We expressed two different 6XHis-tagged Sm14 fusion proteins in a T7 promoter based plasmids. The 6XHis-tag fusions allowed rapid purification of the recombinant proteins through a Ni+2-charged resin. The resulted recombinant 18 and 16 kDa proteins were recognized by anti-Sm14 antibodies and also by antiserum against adult S. mansoni soluble secreted/excreted proteins in Western-Blot. Both proteins were also protective against S. mansoni cercariae infection to the same extent as the rSm14 expressed by the pGEMEX system