B4GALNT2 transferase activity revealed by DBA lectin after in vitro overexpression of <i>B4GALNT2</i> in ovine granulosa cells.

<p>Primary ovine granulosa cells from <i>+/+</i> small antral follicles were transiently transfected with either the pCDNA-hB4GALNT2 expressing the human form of B4GALNT2 or the empty pCDNA3.1 vector. Twenty-four hours after transfection, cells were stained with biotinylated-DBA lectin (500 ng/ml). Arrows indicated DBA positive staining only in B4GALNT2 transfected cells. Cells were counterstained with hematoxylin. A black bar indicates the microscopy magnification scale.</p

Polymorphisms within the minimal <i>FecL</i> locus and allele sharing.

<p>Polymorphism positions are given relative to OAR11 v3.1 and <i>FecL</i> locus (Genbank:KC352617). Polymorphism type, single nucleotide polymorphism (SNP), microsatellite (Ms) or insertion/deletion (ins/del) referred to <i>+/+</i> vs. <i>L/L</i> sequence. Allele sharing indicated number of (<i>+</i>) chromosomes carrying the (<i>L</i>) allele relative to the number of (<i>+</i>) chromosomes tested.</p>a<p>SNP corresponding to the interval borders and therefore excluded from the minimal locus.</p>b<p>Allele segregating as the <i>FecL^L</i> mutation.</p>c<p>Ms marker genotyped by fluorescent SSCP, no information on the dinucleotide repeat variation.</p><p>NA, non-available coordinate on OARv3.1.</p

Western immunoblotting analysis of B4GALNT2 transferase activity in Lacaune sheep granulosa cells and follicular fluids.

<p>Granulosa cell protein extracts (50 µg) and follicular fluids (200 µg) from <i>+/+</i> and <i>L/L</i> large antral follicles were precipitated (P) by agarose-DBA lectin or sepharose-protein A-KM694 monoclonal antibody. The resulting purified glycoproteins were separated on SDS-PAGE, transferred on nitrocellulose membrane and revealed after blotting (B) using biotinylated-DBA lectin or KM694 monoclonal antibody.</p

DBA-purified proteins present in L/L follicular fluid identified by mass spectrometry.

<p>DBA-purified proteins present in L/L follicular fluid identified by mass spectrometry.</p

Quantitative PCR primer sequences and their efficiency.

<p>Quantitative PCR primer sequences and their efficiency.</p

B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary.

<p>Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with biotinylated-DBA lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.</p

Immunostaining for B4GALNT2 in Lacaune sheep ovary.

<p>Photomicrographs of ovarian sections from +/+ and L/L ewes stained with anti-B4GALNT2 rabbit polyclonal antibody (1/50 dilution). Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.</p

Mares FSIGT

Data from the Frequently Sampled Intravenous Glucose Tolerance Test (FSIGT) performed in mares at 300 days of gestation. AIRg=Acute pancras response, DI=Disposition index, SI=Insulin sensitivity, Sg=Glucose effectivness, GB=Basal glucose, IB=Basal insulin

Foals FSIGT

Data from the Frequently Sampled Intravenous Glucose Tolerance Test (FSIGT) performed in growing foals at 180, 360 and 540 days of age. AIRg=Acute pancras response, DI=Disposition index, SI=Insulin sensitivity, Sg=Glucose effectivness, GB=Basal glucose, IB=Basal insulin

Nutritional data of pregnant mares

Energy (HFU=Horse Feed Unit (1HFU=2250kcal)), proteins (HDCP=Horse Difestible Crude Proteins), Fibres (RC=Raw Cellulose), calcium (Ca), phosphorus (P) and calcium to phosphorus ration (Ca_P) ingested by mares between 180 and 330 days of gestation