Photometric and spectroscopic variability of 53 Per

A new investigation of the variability of the SPB-type star 53 Per is presented. The analysis of the BRITE photometry allowed us to determine eight independent frequencies and the combination one. Five of these frequencies and the combination one were not known before. In addition, we gathered more than 1800 new moderate and high-resolution spectra of 53 Per spread over approximately six months. Their frequency analysis revealed four independent frequencies and the combination one, all consistent with the BRITE results.Comment: 2 pages, accepted for publication in the Proceedings of the PAS (Proc. of the 2nd BRITE Science conference, Innsbruck

Marine anthraquinones: Pharmacological and toxicological issues

The marine ecosystem, populated by a myriad of animals, plants, and microorganisms, is an inexhaustible reservoir of pharmacologically active molecules. Among the multiple secondary metabolites produced by marine sources, there are anthraquinones and their derivatives. Besides being mainly known to be produced by terrestrial species, even marine organisms and the uncount-able kingdom of marine microorganisms biosynthesize anthraquinones. Anthraquinones possess many different biological activities, including a remarkable antitumor activity. However, due to their peculiar chemical structures, anthraquinones are often associated with toxicological issues, even relevant, such as genotoxicity and mutagenicity. The aim of this review is to critically describe the an-ticancer potential of anthraquinones derived from marine sources and their genotoxic and mutagenic potential. Marine-derived anthraquinones show a promising anticancer potential, although clinical studies are missing. Additionally, an in-depth investigation of their toxicological profile is needed before advocating anthraquinones as a therapeutic armamentarium in the oncological area

Na-O anticorrelation and HB. IX. Kinematics of the program clusters. A link between systemic rotation and HB morphology?

We use accurate radial velocities for 1981 member stars in 20 Galactic globular clusters, collected within our large survey aimed at the analysis of the Na-O anti-correlation, to study the internal kinematics of the clusters. We performed the first systematic exploration of the possible connections between cluster kinematics and the multiple populations phenomenon in GCs. We did not find any significant correlation between Na abundance and either velocity dispersion or systemic rotation. We searched for systemic rotation in the eight clusters of our sample that lack such analysis from previous works in the literature (NGC2808, NGC5904, NGC6171, NGC6254, NGC6397, NGC6388, NGC6441, and NGC6838). These clusters are found to span a large range of rotational amplitudes, from ~0.0 km/s (NGC6397) to ~13.0 km/s (NGC6441). We found a significant correlation between the ratio of rotational velocity to central velocity dispersion (V_{rot}/sigma_0) and the Horizontal Branch Morphology parameter (B-R)/(B+R+V). V_{rot}/sigma_0 is found to correlate also with metallicity, possibly hinting to a significant role of dissipation in the process of formation of globular clusters. V_{rot} is found to correlate well with (B-R)/(B+R+V), M_V, sigma_0 and [Fe/H]. All these correlations strongly suggest that systemic rotation may be intimately linked with the processes that led to the formation of globular clusters and the stellar populations they host.Comment: Accepted for publication on Astronomy & Astrophysics. Pdflatex, 16 pages, 16 pdf figures. The position angles of the rotation axes have been corrected, since the values reported in the previous version were erroneous. The results of the analysis are unchanged. The manuscript has also been processed by a language edito

Making and Sharing the Commons: Reimagining ‘the West’ as Riverlands, Sydney through a Dialogue between Design and Ethnography

Scholars from the social sciences and humanities are increasingly seeking to improve the relevance and social impact of their research beyond the academy. In this context, ‘designerly’ thinking and methods are being drawn on to inform social change agendas, and a range of new relationships and collaborations are forming around this node of activity. This article critically reflects on this trajectory through a dialogue between ethnography, design and theoretical principles from anthropology and human geography

A fixed combination of probiotics and herbal extracts attenuates intestinal barrier dysfunction from inflammatory stress in an in vitro model using Caco-2 cells.

Background: Inflammatory Bowel Diseases (IBD), are considered a growing global disease, with about ten million people being affected worldwide. Maintenance of intestinal barrier integrity is crucial for preventing IBD onset and exacerbations. Some recent patents regarding oily formulations containing probiotics (WO2010122107A1 and WO2010103374A9) and the use of probiotics for gastrointestinal complaints (US20110110905A1 and US9057112B2) exist, or are pending application. Objective: In this work, we studied the effect of a fixed combination of registered Lactobacillus reuteri and Lactobacillus acidophilus strains and herbal extracts in an in vitro inflammation experimental model. Methods: Caco-2 cell monolayer was exposed to INF-\u3b3+TNF-\u3b1 or to LPS; Trans Epithelial Electrical Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin Tight Junctions (TJs) were also investigated by mean of immunofluorescence. Results: Pre-treatment with the fixed combination of probiotics and herbal extracts prevented the inflammation-induced TEER decrease, paracellular permeability increase and TJs translocation. Conclusions: In summary, the fixed combination of probiotics and herbal extracts investigated in this research was found to be an interesting candidate for targeting the re-establishment of intestinal barrier function in IBD conditions

Antitumor Potential of Marine and Freshwater Lectins

Often, even the most effective antineoplastic drugs currently used in clinic do not efficiently allow complete healing due to the related toxicity. The reason for the toxicity lies in the lack of selectivity for cancer cells of the vast majority of anticancer agents. Thus, the need for new potent anticancer compounds characterized by a better toxicological profile is compelling. Lectins belong to a particular class of non-immunogenic glycoproteins and have the characteristics to selectively bind specific sugar sequences on the surface of cells. This property is exploited to exclusively bind cancer cells and exert antitumor activity through the induction of different forms of regulated cell death and the inhibition of cancer cell proliferation. Thanks to the extraordinary biodiversity, marine environments represent a unique source of active natural compounds with anticancer potential. Several marine and freshwater organisms, ranging from the simplest alga to the most complex vertebrate, are amazingly enriched in these proteins. Remarkably, all studies gathered in this review show the impressive anticancer effect of each studied marine lectin combined with irrelevant toxicity in vitro and in vivo and pave the way to design clinical trials to assess the real antineoplastic potential of these promising proteins. It provides a concise and precise description of the experimental results, their interpretation as well as the experimental conclusions that can be drawn

Sonic hedgehog medulloblastoma cancer stem cells mirnome and transcriptome highlight novel functional networks

Molecular classification has improved the knowledge of medulloblastoma (MB), the most common malignant brain tumour in children, however current treatments cause severe side effects in patients. Cancer stem cells (CSCs) have been described in MB and represent a sub population characterised by self-renewal and the ability to generate tumour cells, thus representing the reservoir of the tumour. To investigate molecular pathways that characterise this sub population, we isolated CSCs from Sonic Hedgehog Medulloblastoma (SHH MB) arisen in Patched 1 (Ptch1) heterozygous mice, and performed miRNA-and mRNA-sequencing. Comparison of the miRNA-sequencing of SHH MB CSCs with that obtained from cerebellar Neural Stem Cells (NSCs), allowed us to obtain a SHH MB CSC miRNA differential signature. Pathway enrichment analysis in SHH MB CSCs mirnome and transcriptome was performed and revealed a series of enriched pathways. We focused on the putative targets of the SHH MB CSC miRNAs that were involved in the enriched pathways of interest, namely pathways in cancer, PI3k-Akt pathway and protein processing in endoplasmic reticulum pathway. In silico analysis was performed in SHH MB patients and identified several genes, whose expression was associated with worse overall survival of SHH MB patients. This study provides novel candidates whose functional role should be further investigated in SHH MB

The normal chemistry of multiple stellar populations in the dense globular cluster NGC 6093 (M 80)

We present the abundance analysis of 82 red giant branch stars in the dense, metal-poor globular cluster NGC 6093 (M 80), the largest sample of stars analyzed in this way for this cluster. From high resolution UVES spectra of 14 stars and intermediate resolution GIRAFFE spectra for the other stars we derived abundances of O, Na, Mg, Al, Si, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Y, Zr, Ba, La, Ce, Pr, Nd, Sm, Eu. On our UVES metallicity scale the mean metal abundance of M 80 is [Fe/H]=-1.791+/-0.006+/-0.076 (+/-statistical +/-systematic error) with rms=0.023 (14 stars). M 80 shows star to star variations in proton-capture elements, and the extension of the Na-O anticorrelation perfectly fit the relations with (i) total cluster mass, (ii) horizontal branch morphology, and (iii) cluster concentration previously found by our group. The chemistry of multiple stellar populations in M 80 does not look extreme. The cluster is also a typical representative of halo globular clusters for what concerns the pattern of alpha-capture and Fe-group elements. However we found that a significant contribution from the s-process is required to account for the distribution of neutron-capture elements. A minority of stars in M 80 seem to exhibit slightly enhanced abundances of s-process species, compatible with those observed in M 22 and NGC 1851, although further confirmation from larger samples is required.Comment: 18 pages, 21 figures, 10 tables; accepted for publication on Astronomy and Astrophysic

NGC 362: another globular cluster with a split red giant branch

We obtained FLAMES GIRAFFE+UVES spectra for both first and second-generation red giant branch (RGB) stars in the globular cluster (GC) NGC 362 and used them to derive abundances of 21 atomic species for a sample of 92 stars. The surveyed elements include proton-capture (O, Na, Mg, Al, Si), alpha-capture (Ca, Ti), Fe-peak (Sc, V, Mn, Co, Ni, Cu), and neutron-capture elements (Y, Zr, Ba, La, Ce, Nd, Eu, Dy). The analysis is fully consistent with that presented for twenty GCs in previous papers of this series. Stars in NGC 362 seem to be clustered into two discrete groups along the Na-O anti-correlation, with a gap at [O/Na] 0 dex. Na-rich, second generation stars show a trend to be more centrally concentrated, although the level of confidence is not very high. When compared to the classical second-parameter twin NGC 288, with similar metallicity, but different horizontal branch type and much lower total mass, the proton-capture processing in stars of NGC 362 seems to be more extreme, confirming previous analysis. We discovered the presence of a secondary RGB sequence, redder than the bulk of the RGB: a preliminary estimate shows that this sequence comprises about 6% of RGB stars. Our spectroscopic data and literature photometry indicate that this sequence is populated almost exclusively by giants rich in Ba, and probably rich in all s-process elements, as found in other clusters. In this regards, NGC 362 joins previously studied GCs like NGC 1851, NGC 6656 (M 22), and NGC 7089 (M 2).Comment: 16 pages, 23 figures, 11 tables, accepted for publication on Astronomy and Astrophysic