Development Of A Computer Program To Improve Sixth Graders Use Of A Local Area Network

This practicum was developed in response to a concern that a week-long Orientation Program did not eliminate the difficulties experienced by sixth graders signing- on the school’s Local Area Network. Class printouts demonstrated a noticeable discrepancy between the number of skills students were programmed to complete and the actual number completed. To remedy this situation, a computer program was developed to introduce practicum participants to networking; to explain the signing-on process in a clear, concise way; and to demonstrate the proper use of the computer control keys needed to successfully use the networking software. Results showed marked improvement in the use of the LAN by those students who viewed the program complement. This tutorial is now recognized as an integral part in the implementation of the school\u27s networking program. Appendices include a Pre/Post Test, charts to demonstrate progress made, glossary, and computer printout of the program complement

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field