Enhancement of Maritime Safety and Economic Benefits of Short Sea Shipping Ship Routing

The relevance of ship routing system is increasing according to the mitigation of carbon emissions and enhance the maritime safety. New generation of high-resolution meteo-oceanographic predictions provides useful tools for routing of ship. However, scientific efforts have been focused on inter-oceanic routes. This contribution investigates the economic benefits and improvement on maritime safety of ship routing of Short Sea Shipping (SSS) routes. The investigation is supported with the development of a ship routing system based on a path finding algorithm and meteo-oceanographic predications. Results show that the economic benefits using ship routing in SSS is estimated in percentage of the total cost during energetic wave episodes. The work establishes the basis of further developments in optimal route applied in relatively short-distances and its systematic use in the SSS maritime industry. In this work, the optimal ship routing analysis is investigated in a relative short distance maritime route between Barcelona and Palma de Mallorca (Spain). Dijkstra algorithm is implemented in order to obtain the optimal path under an energetic wave event. The methodology is based on the inclusion of the drag resistance due to waves. The results reveal how the wave direction has a relevant role in the optimum path due to the relative direction with the ship and the enhancement of the navigational safety.Postprint (published version

Ship emissions reduction using weather ship routing optimisation

A significant proportion of global carbon dioxide emissions are attributed to ocean-sailing ships and shipping emissions are predicted to double in less than 30 years. This paper investigates the benefit of using weather ship routing optimisation, assessing the ship emissions for minimum distance routes and optimised routes. A heuristic pathfinding algorithm is used to obtain the minimum cost (i.e. optimised route) in terms of sailing time, using high-resolution wave forecasting. The assessment of fuel consumption and ship emissions calculations were inspired by the STEAM2 bottom-up approach, in conjunction with the estimation of the power increase needed to overcome speed decrement due to waves. Several scenarios covering the Western Mediterranean Short Sea Shipping routes (from 24 to 600 nautical miles and using a real Ro-Pax vessel) are compared in terms of emissions between the minimum distance route and the optimum. The ship routing optimisation reveals a reduction up to 30% of ship emissions during severe storms on longer routes. Nevertheless, all the cases studied show emissions mitigation when ship routing optimisation is used. The expected increase of extreme weather events, in terms of frequency, intensity and duration due to climate change, suggests a gradual gain of implementing weather ship routing optimisation in all types of routes, regardless of the distance.Postprint (author's final draft

A comprehensive ship weather routing system using CMEMS products and A* algorithm

We describe the implementation of a comprehensive software for Ship Weather Routing referred to as SIM- ROUTE. The A* pathfinding algorithm is used to optimize the sailing route as a function of the wave action. The aim of the software is to provide a comprehensive, open and easy tool including pre- and post-processing for ship weather routing simulations. The software is constructed considering the Copernicus Marine Environment Monitoring Service (CMEMS) wave predictions systems which are available for free use. The code provides the optimized route and the minimum distance route together with additional modules to compute ship emission and safety on navigation monitoring. SIMROUTE has been tested in several cases using different CMEMS products over short and long distances. The comprehensive structure of the code enables it to be easily modified to include additional ship wave resistance models and the effect of the water currents and winds on navigation. SIMROUTE is also used for academic purposes, providing skills for ship routing optimization in the framework of standards of training, certification and watchkeeping (STCW) for competence-based maritime education and training. Due to the simplicity of its use, SIMROUTE is a good candidate for benchmarking strategies and inter-comparison exercises with advanced methods for ship weather routing. This contribution highlights the technical aspects, code organization and structure behind SIMROUTE, demonstrating its capabilities through examples of route optimization.Postprint (published version

Ship weather routing using pathfinding algorithms: the case of Barcelona – Palma de Mallorca

Weather ship routing has become a recognized measure to target safe, sustainable and economical ship activities. Academic research has focused the ship routing optimization through pathfinding algorithms which take into account the meteo-oceanographic forecasts (i.e. wind, waves or currents predictions). This contribution shows the results of the numerical simulations carried out during the development of a weather ship routing applied to a ferry service in the Mediterranean Sea: Barcelona – Palma de Mallorca. From a methodological point of view, the pathfinding A* algorithm is applied to optimize the travel time considering the wave action. Under severe weather conditions, a reduction of the 6% of the travel time is obtained comparing the optimized route and the minimum distance route. The results show also a non-significant correlation between the travel time reduction and wave height. In consequence the benefit of ship routing depends not only of the wave height but also in the spatial sequence of the storm.Postprint (published version

Semen miRNAs Contained in Exosomes as Non-Invasive Biomarkers for Prostate Cancer Diagnosis

Although it is specific for prostatic tissue, serum prostate-specific antigen (PSA) screening has resulted in an over-diagnosis of prostate cancer (PCa) and many unnecessary biopsies of benign disease due to a well-documented low cancer specificity, thus improvement is required. We profiled the expression level of miRNAs contained in semen exosomes from men with moderately increased PSA levels to assess their usefulness, either alone or in addition to PSA marker, as non-invasive biomarkers, for the early efficient diagnosis and prognosis of PCa. An altered miRNA expression pattern was found by a high throughput profiling analysis in PCa when compared with healthy individuals (HCt) exosomal semen samples. The presence of vasectomy was taken into account for the interpretation of results. Fourteen miRNAs were selected for miRNA validation as PCa biomarkers in a subsequent set of semen samples. In this explorative study, we describe miRNA-based models, which included miRNA expression values together with PSA levels, that increased the classification function of the PSA screening test with diagnostic and/or prognostic potential: [PSA miR-142-3p miR-142-5p miR-223-3p] model (AUC:0,821) to discriminate PCa from BPH (Sn:91,7% Sp:42,9% vs Sn:100%Sp:14,3%); and [PSA miR-342-3p +/- miR-374b-5p] model (AUC: 0,891) to discriminate between GS >= 7 tumours and men presenting PSA >= 4 ng/ml with no cancer or GS6 tumours (Sn:81,8% Sp:95% vs Sn:54,5% Sp:90%). The pathway analysis of predicted miRNA target genes supports a role for these miRNAs in PCa aetiology and/or progression. Our study shows semen exosome miRNA-based models as molecular biomarkers with the potential to improve PCa diagnosis/prognosis efficiency. As the next step, further prospective studies on larger cohorts of patients are required to validate the diagnostic and/or prognostic role of the miRNA panel before it could be adopted into clinical practice

Annual General Assembly of the International Association of Maritime Universities

978-84-947311-7-

Ship weather routing using pathfinding algorithms: the case of Barcelona – Palma de Mallorca

Weather ship routing has become a recognized measure to target safe, sustainable and economical ship activities. Academic research has focused the ship routing optimization through pathfinding algorithms which take into account the meteo-oceanographic forecasts (i.e. wind, waves or currents predictions). This contribution shows the results of the numerical simulations carried out during the development of a weather ship routing applied to a ferry service in the Mediterranean Sea: Barcelona – Palma de Mallorca. From a methodological point of view, the pathfinding A* algorithm is applied to optimize the travel time considering the wave action. Under severe weather conditions, a reduction of the 7% of the travel time is obtained comparing the optimized route and the minimum distance route. The results show also a non-significant correlation between the travel time reduction and wave height. In consequence the benefit of ship routing depends not only of the wave height but also in the spatial sequence of the storm

Ship-weather routing applied to short sea distances: study of the feasibility of SIMROUTEv2 algorithm

Postprint (author's final draft

Impact of Extracellular Vesicle Isolation Methods on Downstream miRNA Analysis in Semen: A Comparative Study

Seminal plasma (SP) contains a unique concentration of miRNA, mostly contained in small extracellular vesicles (sEVs) such as exosomes, some of which could be clinically useful for diagnosis and/or prognosis of urogenital diseases such as prostate cancer (PCa). We optimized several exosome-EV isolation technologies for their use in semen, evaluating EV purifying effectiveness and impact on the downstream analysis of miRNAs against results from the standard ultracentrifugation (UC) method to implement the use of SP sEV_miRNAs as noninvasive biomarkers for PCa. Our results evidenced that commercial kits designed to isolate exosomes/EVs from blood or urine are mostly applicable to SP, but showed quantitative and qualitative variability between them. ExoGAG 3500× g and the miRCURY Cell/Urine/CSF 1500× g methods resulted as equivalent alternative procedures to UC for isolating exosomes/sEVs from semen for nanoparticle characteristics and quality of RNA contained in vesicles. Additionally, the expression profile of the altered semen sEV-miRNAs in PCa varies depending on the EV isolation method applied. This is possibly due to different extraction techniques yielding different proportions of sEV subtypes. This is evidence that the exosome-EV isolation method has a significant impact on the analysis of the miRNAs contained within, with important consequences for their use as clinical biomarkers. Therefore, miRNA analysis results for EVs cannot be directly extrapolated between different EV isolation methods until clear markers for delineation between microvesicles and exosomes are established. However, EV extraction methodology affects combined models (semen exosome miRNA signatures plus blood Prostate specific antigen (PSA) concentration for PCa diagnosis) less; specifically our previously described (miR-142-3p + miR-142-5p + miR-223-3p + PSA) model functions as molecular marker from EVs from any of the three isolation methods, potentially improving the efficiency of PSA PCa diagnosis

Serum methylation of GALNT9, UPF3A, WARS, and LDB2 as noninvasive biomarkers for the early detection of colorectal cancer and advanced adenomas

Background Early detection has proven to be the most effective strategy to reduce the incidence and mortality of colorectal cancer (CRC). Nevertheless, most current screening programs suffer from low participation rates. A blood test may improve both the adherence to screening and the selection to colonoscopy. In this study, we conducted a serum-based discovery and validation of cfDNA methylation biomarkers for CRC screening in a multicenter cohort of 433 serum samples including healthy controls, benign pathologies, advanced adenomas (AA), and CRC.Results First, we performed an epigenome-wide methylation analysis with the MethylationEPIC array using a sample pooling approach, followed by a robust prioritization of candidate biomarkers for the detection of advanced neoplasia (AN: AA and CRC). Then, candidate biomarkers were validated by pyrosequencing in independent individual cfDNA samples. We report GALNT9, UPF3A, WARS, and LDB2 as new noninvasive biomarkers for the early detection of AN. The combination of GALNT9/UPF3A by logistic regression discriminated AN with 78.8% sensitivity and 100% specificity, outperforming the commonly used fecal immunochemical test and the methylated SEPT9 blood test.Conclusions Overall, this study highlights the utility of cfDNA methylation for CRC screening. Our results suggest that the combination methylated GALNT9/UPF3A has the potential to serve as a highly specific and sensitive blood-based test for screening and early detection of CRC