Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels

Greenhouse gas emissions, such as N2O from excessive N-fertilizer use, are of concern. Symbiotic N2-fixation by pulses such as soybean might mitigate this issue, for which inoculants carrying locally adapted Bradyrhizobium strains are recommended. In the frame of this goal, enhancing the quality control of these inoculants is required on two key aspects: determining the presence of nosZ, for the strains being able to reduce N2O, and assessing N2-fixation potential. Previously it was demonstrated that, in soybean leaves, N-contents are well correlated with chlorophyll contents. However, no such correlations were made with either N obtained from N2-fixation or with nodules mass, which is an indicator of nodular activity. Here we aimed to leverage the correlation between N and chlorophyll levels to develop a simple and non-destructive laboratory method to be applied in quality control of inoculants, able to assess the N2-fixing capacity of rhizobial strains. To establish such correlations, we cultivated soybeans in vermiculite with N-free nutrient solution, and inoculated them with various Bradyrhizobium field isolates that displayed a range of symbiotic N2-fixing capacities. Subsequently, we measured chlorophyll with a portable chlorophyllometer, and correlated these measures with symbiotic parameters. Moreover, we tested for the presence of nosZ by PCR. We observed significant correlations between chlorophyll and shoot nitrogen obtained from N2-fixation and, in addition, we corroborated that chlorophyll contents were significantly correlated also with nodules mass. Two B. diazoefficiens strains stood out and possessed nosZ. In contrast, B. elkanii and B. japonicum isolates displayed lower chlorophyll and symbiotic performance, and lacked nosZ. Our findings highlight the potential of measuring chlorophyll contents and testing for the presence of nosZ as two straightforward techniques that may enhance laboratory tests for quality control, enabling selection of superior and safe locally isolated strains for soybean inoculants without increased production costs

Una estrategia para la internacionalización de PyMEs argentinas de maquinaria agrícola : el Clúster Empresarial CIDETER de Maquinaria Agrícola.

Desde sus comienzos, la industria nacional de la maquinaria agrícola ha estado orientada, fundamentalmente, a satisfacer la demanda de implementos en el mercado interno, lo cual dio lugar a una dinámica productiva atada a los ciclos económicos internos marcados por la inestabilidad. Es dentro del marco descripto, que las empresas, instituciones y organismos vinculados al sector se proponen implementar una estrategia para la internacionalización de la maquinaria agrícola de origen nacional. El siguiente trabajo se propone analizar la estrategia de internacionalización del Clúster Empresarial CIDETER de la Maquinaria Agrícola, sus instrumentos y el impacto que esta ha tenido en la inserción internacional del sector. El análisis de las acciones implementadas así como también, la observación de las estadísticas referidas a exportaciones, permiten dilucidar que la estrategia contribuyó a la internacionalización del sector aunque hubo otros aspectos que acompañaron el proceso, tales como el alza en los precios de commodities y los acuerdos de cooperación con Venezuela.Since its inception, the Argentinian agricultural machinery industry has been mainly oriented to satisfying domestic market needs, which created a productive dynamic tied to economics cycles marked by instability. Within this context, companies, institutions, and organizations linked to this sector intend to implement a strategy for the internationalization of local agricultural machinery. The following paper aims to analyze the internationalization strategy of Agricultural Machinery Cluster, its instruments and its impact on the international insertion of the sector. The analysis of the actions implemented as well as the statistics related to exports, allow us to say that the strategy fulfilled its objective although there were other aspects that accompanied the process, such as the rise in commodities prices and cooperation agreements with Venezuela.Fil: Castells, Damián. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales; Argentina

High throughput method to characterize acid-base properties of insoluble drug candidates in water

In drug design experimental characterization of acidic groups in candidate molecules is one of the more important steps prior to the in-vivo studies. Potentiometry combined with Yasuda-Shedlovsky extrapolation is one of the more important strategy to study drug candidates with low solubility in water, although, it requires a large number of sequences to determine pKa values at different solvent-mixture compositions to, finally, obtain the pKa in water (pwwKa) by extrapolation. We have recently proposed a method which requires only two sequences of additions to study the effect of organic solvent content in liquid chromatography mobile phases on the acidity of the buffer compounds usually dissolved in it along wide ranges of compositions. In this work we propose to apply this method to study thermodynamic pwwKa of drug candidates with low solubilities in pure water. Using methanol/water solvent mixtures we study six pharmaceutical drugs at 25 °C. Four of them: ibuprofen, salicylic acid, atenolol and labetalol, were chosen as members of carboxylic, amine and phenol families, respectively. Since these compounds have known pwwKa values, they were used to validate the procedure, the accuracy of Yasuda-Shedlovsky and other empirical models to fit the behaviors, and to obtain pwwKa by extrapolation. Finally, the method is applied to determine unknown thermodynamic pwwKa values of two pharmaceutical drugs: atorvastatin calcium and the two dissociation constants of ethambutol. The procedure proved to be simple, very fast and accurate in all of the studied cases.Fil: Benito, Damián Ezequiel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Tecnología de Recursos Minerales y Cerámica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Tecnología de Recursos Minerales y Cerámica; ArgentinaFil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Gagliardi, Leonardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentin

Original datasets for "Quality control of Bradyrhizobium inoculant strains: Detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels"

This dataset comprises the original data collected in nodulation experiments carried out for the publication "Quality control of Bradyrhizobium inoculant strains: Detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels" (see "Related documents").Para acceder al artículo al cual pertenece este conjunto de datos, hacer clic en "Documentos relacionados".Fil: Brignoli, Damián. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Frickel Critto, Emilia Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Sandobal, Tamara Jesabel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Balda, Rocío Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Mongiardini, Elías Javier. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Pérez Giménez, Julieta, Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. ArgentinaInstituto de Biotecnología y Biología Molecula

Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels

Greenhouse gas emissions, such as N2O from excessive N-fertilizer use, are of concern. Symbiotic N2-fixation by pulses such as soybean might mitigate this issue, for which inoculants carrying locally adapted Bradyrhizobium strains are recommended. In the frame of this goal, enhancing the quality control of these inoculants is required on two key aspects: determining the presence of nosZ, for the strains being able to reduce N2O, and assessing N2- fixation potential. Previously it was demonstrated that, in soybean leaves, Ncontents are well correlated with chlorophyll contents. However, no such correlations were made with either N obtained from N2-fixation or with nodules mass, which is an indicator of nodular activity. Here we aimed to leverage the correlation between N and chlorophyll levels to develop a simple and non-destructive laboratory method to be applied in quality control of inoculants, able to assess the N2-fixing capacity of rhizobial strains. To establish such correlations, we cultivated soybeans in vermiculite with N-free nutrient solution, and inoculated them with various Bradyrhizobium field isolates that displayed a range of symbiotic N2-fixing capacities. Subsequently, we measured chlorophyll with a portable chlorophyllometer, and correlated these measures with symbiotic parameters. Moreover, we tested for the presence of nosZ by PCR. We observed significant correlations between chlorophyll and shoot nitrogen obtained from N2-fixation and, in addition, we corroborated that chlorophyll contents were significantly correlated also with nodules mass. Two B. diazoefficiens strains stood out and possessed nosZ. In contrast, B. elkanii and B. japonicum isolates displayed lower chlorophyll and symbiotic performance, and lacked nosZ. Our findings highlight the potential of measuring chlorophyll contents and testing for the presence of nosZ as two straightforward techniques that may enhance laboratory tests for quality control, enabling selection of superior and safe locally isolated strains for soybean inoculants without increased production costs.Puede accederse al conjunto de datos relacionado a este trabajo haciendo clic en "Documentos relacionados".Instituto de Biotecnología y Biología MolecularLaboratorio de Investigación y Desarrollo de Métodos AnalíticosComisión de Investigaciones Científicas de la provincia de Buenos Aire

Educación Superior y Género: Una aproximación desde la UNR

Nos acercamos en esta oportunidad para compartir la tercera producción del Programa Género y Universidad de la Universidad Nacional de Rosario, denominada “Educación Superior y Género: Una aproximación desde la UNR”. Una gran red temática que apunta a la información, la reflexión y la apropiación, conformando un espacio nodal de interacción, para ser una propuesta de trabajo en cada espacio de acción.Universidad Nacional de Rosari