22 research outputs found

    Bdellovibrio bacteriovorus Inhibits Staphylococcus aureus Biofilm Formation and Invasion into Human Epithelial Cells

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    Bdellovibrio bacteriovorus HD100 is a predatory bacterium that attacks many Gram-negative human pathogens. A serious drawback of this strain, however, is its ineffectiveness against Gram-positive strains, such as the human pathogen Staphylococcus aureus. Here we demonstrate that the extracellular proteases produced by a host-independent B. bacteriovorus (HIB) effectively degrade/inhibit the formation of S. aureus biofilms and reduce its virulence. A 10% addition of HIB supernatant caused a 75% or greater reduction in S. aureus biofilm formation as well as 75% dispersal of pre-formed biofilms. LC-MS-MS analyses identified various B. bacteriovorus proteases within the supernatant, including the serine proteases Bd2269 and Bd2321. Tests with AEBSF confirmed that serine proteases were active in the supernatant and that they impacted S. aureus biofilm formation. The supernatant also possessed a slight DNAse activity. Furthermore, treatment of planktonic S. aureus with the supernatant diminished its ability to invade MCF-10a epithelial cells by 5-fold but did not affect the MCF-10a viability. In conclusion, this study illustrates the hitherto unknown ability of B. bacteriovorus to disperse Gram-positive pathogenic biofilms and mitigate their virulence.open6

    Gendered experiences in teaching: an exploration into the participation and needs of female teachers in deprived rural areas of Ghana

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    Research in Ghana suggests that very few female teachers accept and stay in teaching posts in rural deprived areas. This article is based on a study conducted across six districts in three diverse geographic zones of Ghana with the lowest percentage of female teachers. The article provides evidence as to why the Government of Ghana has found it difficult to post female teachers to rural areas and the challenges which female teachers face once serving in these areas. The article also explores the strategies which could be used to attract more teachers to these areas and provides a framework for investigating the nature of posting women and the implications for the girl child.RĂ©sumĂ©Des recherches menĂ©es au Ghana laissent croire que trĂšs peu d’enseignantes acceptent d’exercer ou de vivre dans les rĂ©gions rurales dĂ©favorisĂ©es du pays. Cet article est le fruit d’une Ă©tude menĂ©e dans six districts situĂ©s dans trois zones gĂ©ographiques diffĂ©rentes du Ghana qui ont le plus bas pourcentage d’enseignantes. L'article propose des preuves pour expliquer pourquoi les autoritĂ©s ghanĂ©ennes ont souvent du mal Ă  affecter des enseignantes en zone rurale et souligne aussi les difficultĂ©s auxquelles font face des enseignantes dans ces rĂ©gions dĂ©favorisĂ©es. L'article explore Ă©galement les stratĂ©gies Ă  adopter pour attirer davantage d’enseignantes dans ces rĂ©gions tout en proposant un cadre conceptuel pour l’analyse de la spĂ©cificitĂ© de l’affectation des femmes et de ses implications pour la formation des petites filles

    Education, culture and development in Northern Ghana Micro realities and macro context; implications for policy and practice

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    SIGLEAvailable from British Library Document Supply Centre-DSC:DXN031556 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
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