Picosecond electrical spectroscopy using monolithic GaAs circuits

This article describes an experimental apparatus for free-space mm-wave transmission measurements (spectroscopy). GaAs nonlinear transmission lines and sampling circuits are used as picosecond pulse generators and detectors, with planar monolithic bowtie antennas with associated substrate lenses used as the radiating and receiving elements. The received pulse is 270 mV amplitude and 2.4 ps rise time. Through Fourier transformation of the received pulse, 30–250 GHz measurements are demonstrated with <=0.3 dB (rms) accuracy

Wide-angle, circularly polarized, omnidirectional-array antenna

Modified conventional turnstile antenna features bifoliate pattern with relatively high gain and good circularity over solid area enclosed by the 0.26 and 1.31 radian angles of elevation. These antennas are intended for high altitude balloon use, their permissible weight is restricted to one pound

Participatory Analysis, Monitoring and Evaluation for Fishing Communities: A Manual

While there are many manuals available on participatory rapid appraisal approaches to monitoring and evaluation, there were none easily used by field officers attempting to aid and encourage fishing community level participation in monitoring and evaluating activities of projects and programmes in rural fishing communities.This manual is prepared in cook book fashion with easily followed instructions for 26 participatory monitoring tools to allow use by both local field staff acting as facilitators and directly by community members engaged in the evaluation process

Dimensional renormalization: ladders to rainbows

Renormalization factors are most easily extracted by going to the massless limit of the quantum field theory and retaining only a single momentum scale. We derive factors and renormalized Green functions to all orders in perturbation theory for rainbow graphs and vertex (or scattering diagrams) at zero momentum transfer, in the context of dimensional renormalization, and we prove that the correct anomalous dimensions for those processes emerge in the limit D -> 4.Comment: RevTeX, no figure

Radio Observations of the Supernova Remnant Candidate G312.5-3.0

The radio images from the Parkes-MIT-NRAO (PMN) Southern Sky Survey at 4850 MHz have revealed a number of previously unknown radio sources. One such source, G312.5-3.0 (PMN J1421-6415), has been observed using the multi-frequency capabilities of the Australia Telescope Compact Array (ATCA) at frequencies of 1380 MHz and 2378 MHz. Further observations of the source were made using the Molonglo Observatory Synthesis Telescope (MOST) at a frequency of 843 MHz. The source has an angular size of 18 arcmin and has a distinct shell structure. We present the reduced multi-frequency observations of this source and provide a brief argument for its possible identification as a supernova remnant.Comment: 5 pages, 5 figures, Accepted for publication in MNRA

On the Vortex-Point Charge Composite: Classical Orbits and Quantum Bound States

The possibility of composite systems arising out of a point charge interacting with a Nielsen-Olesen vortex in 2+1-dimensions is investigated. It is shown that classical bounded orbits are possible for certain ranges of parameters. Long lived metastable states are shown to exist, in a semi-classical approach, from the study of the effective potential. Loss of self-adjointness of the Hamiltonian and its subsequent self-adjoint extension in some cases leads to bound states.Comment: 13 pages, Latex file, For figures e-mail to "[email protected]

Comparative analysis of rigidity across protein families

We present a comparative study in which 'pebble game' rigidity analysis is applied to multiple protein crystal structures, for each of six different protein families. We find that the main-chain rigidity of a protein structure at a given hydrogen bond energy cutoff is quite sensitive to small structural variations, and conclude that the hydrogen bond constraints in rigidity analysis should be chosen so as to form and test specific hypotheses about the rigidity of a particular protein. Our comparative approach highlights two different characteristic patterns ('sudden' or 'gradual') for protein rigidity loss as constraints are removed, in line with recent results on the rigidity transitions of glassy networks

Dominant Negative Effects by Inactive Spa47 Mutants Inhibit T3SS Function and Shigella Virulence

Type three secretion systems (T3SS) are complex nano-machines that evolved to inject bacterial effector proteins directly into the cytoplasm of eukaryotic cells. Many high-priority human pathogens rely on one or more T3SSs to cause disease and evade host immune responses, underscoring the need to better understand the mechanisms through which T3SSs function and their role(s) in supporting pathogen virulence. We recently identified the Shigella protein Spa47 as an oligomerization-activated T3SS ATPase that fuels the T3SS and supports overall Shigella virulence. Here, we provide both in vitro and in vivo characterization of Spa47 oligomerization and activation in the presence and absence of engineered ATPase-inactive Spa47 mutants. The findings describe mechanistic details of Spa47-catalyzed ATP hydrolysis and uncover critical distinctions between oligomerization mechanisms capable of supporting ATP hydrolysis in vitro and those that support T3SS function in vivo. Concentration-dependent ATPase kinetics and experiments combining wild-type and engineered ATPase inactive Spa47 mutants found that monomeric Spa47 species isolated from recombinant preparations exhibit low-level ATPase activity by forming short-lived oligomers with active site contributions from at least two protomers. In contrast, isolated Spa47 oligomers exhibit enhanced ATP hydrolysis rates that likely result from multiple preformed active sites within the oligomeric complex, as is predicted to occur within the context of the type three secretion system injectisome. High-resolution fluorescence microscopy, T3SS activity, and virulence phenotype analyses of Shigella strains co-expressing wild-type Spa47 and the ATPase inactive Spa47 mutants demonstrate that the N-terminus of Spa47, not ATPase activity, is responsible for incorporation into the injectisome where the mutant strains exhibit a dominant negative effect on T3SS function and Shigella virulence. Together, the findings presented here help to close a significant gap in our understanding of how T3SS ATPases are activated and define restraints with respect to how ATP hydrolysis is ultimately coupled to T3SS function in vivo

New measurements of total ionizing dose in the lunar environment

[1] We report new measurements of solar minimum ionizing radiation dose at the Moon onboard the Lunar Reconnaissance Orbiter (LRO) from June 2009 through May 2010. The Cosmic Ray Telescope for the Effects of Radiation (CRaTER) instrument on LRO houses a compact and highly precise microdosimeter whose design allows measurements of dose rates below 1 micro-Rad per second in silicon achieved with minimal resources (20 g, ∼250 milliwatts, and ∼3 bits/second). We envision the use of such a small yet accurate dosimeter in many future spaceflight applications where volume, mass, and power are highly constrained. As this was the first operation of the microdosimeter in a space environment, the goal of this study is to verify its response by using simultaneous measurements of the galactic cosmic ray ionizing environment at LRO, at L1, and with other concurrent dosimeter measurements and model predictions. The microdosimeter measured the same short timescale modulations in the galactic cosmic rays as the other independent measurements, thus verifying its response to a known source of minimum-ionizing particles. The total dose for the LRO mission over the first 333 days was only 12.2 Rads behind ∼130 mils of aluminum because of the delayed rise of solar activity in solar cycle 24 and the corresponding lack of intense solar energetic particle events. The dose rate in a 50 km lunar orbit was about 30 percent lower than the interplanetary rate, as one would expect from lunar obstruction of the visible sky

Darboux Transformations, Infinitesimal Symmetries and Conservation Laws for Nonlocal Two-Dimensional Toda Lattice

The technique of Darboux transformation is applied to nonlocal partner of two-dimensional periodic A_{n-1} Toda lattice. This system is shown to admit a representation as the compatibility conditions of direct and dual overdetermined linear systems with quantized spectral parameter. The generalization of the Darboux transformation technique on linear equations of such a kind is given. The connections between the solutions of overdetermined linear systems and their expansions in series at singular points neighborhood are presented. The solutions of the nonlocal Toda lattice and infinite hierarchies of the infinitesimal symmetries and conservation laws are obtained.Comment: 12 pages, infinitesimal symmetries and conservation laws are adde