The insulin receptor substrate 1 associates with phosphotyrosine phosphatase SHPTP2 in liver and muscle of rats

CAPES – COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOInsulin stimulates the tyrosine kinase activity of its receptor resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate-1 (IRS-1) which, in turn, associates with proteins containing SH2 domains. It has been shown that IRS-1 associates with the tyrosine phosphatase SHPTP2 in cell cultures. While the effect of the IRS-1/SHPTP2 association on insulin signal transduction is not completely known, this association may dephosphorylate IRS-1 and may play a critical role in the mitogenic actions of insulin. However, there is no physiological demonstration of this pathway of insulin action in animal tissues. In the present study we investigated the ability of insulin to induce association between IRS-1 and SHPTP2 in liver and muscle of intact rats, by co-immunoprecipitation with anti-IRS-1 antibody and anti-SHPTP2 antibody. In both tissues there was an increase in IRS-1 association with SHPTP2 after insulin stimulation. This association occurred when IRS-1 had the highest level of tyrosine phosphorylation and the decrease in this association was more rapid than the decrease in IRS-1 phosphorylation levels. The data provide evidence against the participation of SHPTP2 in IRS-1 dephosphorylation in rat tissues, and suggest that the insulin signal transduction pathway in rat tissues is related mainly to the mitogenic effects of the hormone.Insulin stimulates the tyrosine kinase activity of its receptor resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate-1 (IRS-1) which, in turn, associates with proteins containing SH2 domains. It has been shown that IRS-1 associates with the tyrosine phosphatase SHPTP2 in cell cultures. While the effect of the IRS-1/SHPTP2 association on insulin signal transduction is not completely known, this association may dephosphorylate IRS-1 and may play a critical role in the mitogenic actions of insulin. However, there is no physiological demonstration of this pathway of insulin action in animal tissues. In the present study we investigated the ability of insulin to induce association between IRS-1 and SHPTP2 in liver and muscle of intact rats, by co-immunoprecipitation with anti-IRS-1 antibody and anti-SHPTP2 antibody. In both tissues there was an increase in IRS-1 association with SHPTP2 after insulin stimulation. This association occurred when IRS-1 had the highest level of tyrosine phosphorylation and the decrease in this association was more rapid than the decrease in IRS-1 phosphorylation levels. The data provide evidence against the participation of SHPTP2 in IRS-1 dephosphorylation in rat tissues, and suggest that the insulin signal transduction pathway in rat tissues is related mainly to the mitogenic effects of the hormone311114091413CAPES – COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES – COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOsem informaçãosem informaçã

Genotyping Of Kell, Duffy, Kidd And Rhd In Patients With β Thalassemia

Determination of Rh, Kell, Duffy and Kidd phenotypes in addition to ABO is used to prevent the alloimmunization to red blood cells (RBCs) antigens and as part of the antibody identification process in patients with β Thalassemia. However, phenotyping in these patients can be time consuming and difficult to interpret. In these situations, it would be valuable to have an alternative to hemagglutination tests to determine the patient's antigen profile. We used PCR-RFLP to genotype such patients. DNA was prepared from 50 patients with β Thalassemia who had been phenotyped by routine hemagglutination, and tested for Kell, Kidd, Duffy/ GATA mutation by PCR-RFLP. RHD/non-D was analysed by PCR product size associated to RHD gene sequence in intron 4 and exon 10/3'UTR. The genotyping assays were performed without knowledge of phenotype results. For RHD/non-D, 47 were RhD+ and RHD+/RHCE+, and 3 were RhD- and RHD-/RHCE+. For Kell, 48 kk were K2K2 and 2 Kk were K1K2. For Duffy, of 44 samples that had normal GATA box, 8 Fy(a+b-) were FYA/FYA, 15 Fy(a+b+) were FYB/FYB, and 19 Fy(a+b+) were FYA/FYB; of the other 4 samples 3 were FYA/FYB and heterozygous GATA mutation, and 1 Fy(a-b-) was FYB/FYB, homozygous GATA mutation. Two samples phenotyped as Fy(a+b-) that had normal GATA, presented the 265T/298 A mutations and two samples phenotyped as Fy(a-b+) were genotyped was FYA/FYB. For Kidd, 15 Jk(a+b) were JKA/JKA, 12 Jk(a-b+) were JKB/JKB, and 20 Jk(a+b+) were JKA/JKB. Three samples phenotyped as JK(a+b+) were genotyped as JKB/JKB. Genotype is more accurate than phenotype for determination of blood groups in polytransfused patients with β Thalassemia. Genotyping in these patients can be helpful to select antigen-negative RBCs for transfusion.2226976Blumberg, N., Peck, K., Ross, K., Avila, E., Immune response to chronic red blood cell transfusion (1983) Vox Sang, 44, pp. 212-217Economidou, J., Constantoulakis, M., Augoustaki, O., Adinolfi, M., Frequency of antibodies to various antigenic determinants in polytransfused patients with homozygous thalassemia in Greece (1971) Vox Sang, 20, p. 252Sirchia, G., Zanella, A., Parravicini, A., Morelati, F., Rebulla, P., Masera, G., Red cell alloantibodies in patients with thalassemia major. Results of na Italian cooperative study (1985) Transfusion, 25, p. 110Spanos, T., Karageorga, M., Ladis, V., Peristeri, J., Hatziliami, A., Kattamis, C., Red cell alloantibodies in patients with thalassemia (1990) Vox Sang, 58, p. 50Greenwalt, T.J., Zelenski, K.R., Transfusion support for hemoglobinopathies (1984) Clin. Haematol., 13, pp. 151-165Charache, S., Problems in transfusion therapy (1990) N. Engl. J. Med., 322, pp. 1666-1668. , editorialPerkins, H.A., The safety of the blood supply: Making decisions in transfusion medicine (1992) Blood Safety: Current Challenges, pp. 125-150. , Nance SJ, ed. Bethesda: American Association of Blood BanksColes, S.M., Klein, H.G., Holland, P.V., Alloimmunization in two multitransfused patient populations (1981) Transfusion, 21, pp. 462-466Michail-Merianou, V., Pamphili-Panouspoulou, L., Piperi-Lowes, L., Pelegrinis, E., Karaklis, A., Alloimmunization to red cell antigens in thalassemia: Comparative study of usual versus better-match transfusion programmes (1987) Vox Sang, 52, p. 95Reid, M.E., Yazdanbakhsh, K., Molecular insights into blood groups and implications for blood transfusions (1998) Current Opinion in Hematology, 5, pp. 93-102Avent, N.D., Human erythrocyte antigen expression: Its molecular bases (1997) Br. J. Biom. Sci., 54, pp. 16-37Lee, T.H., Donegan, E., Slichter, S., Bush, M.P., Transient increase in circulating donor leucocytes after allogeneic transfusions in Immunocompetent recipients compatible with donor cell proliferation (1995) Blood, 85, pp. 1207-1214Adams, F.T., Davenport, R.D., Rcardon, D.A., Roth, M.S., Detection of circulating donor white blood cells in patients receiving multiple trasnfusions (1992) Blood, 80, pp. 551-555Lee, T.-H., Paglieroni, T., Ohro, H., Holland, P.V., Bush, M.P., Longterm multi-lineage chimerism of donor leucocytes in transfused trauma patients (1996) Blood, 88, p. 265. , abstrRios, M., Cash, K., Strupp, A., Uehlinger, J., Reid, M.E., DNA from urine sediment or buccal cells can be used for blood group molecular genotyping (1999) Immunehematology, 15, pp. 61-65Reid, M.E., Rios, M., Powell, D., Charles-Pierre, D., Malavade, V., DNA from blood samples can be used to genotype patients who have recently received a transfusion (2000) Transfusion, 40, pp. 1-6Davies, L., Dibner, M.D., Battey, J.F., (1986) Basic Methods in Molecular Biology, , Elsevier Science Publishing Co. Inc., New YorkLee, S., Wu, X., Reid, M.E., Zelinski, T., Redman, C., Molecular basis of the Kell (K1) phenotype (1995) Blood, 85, pp. 912-916Olivès, B., Merriman, M., Bailly, P., Bain, S., Barnett, A., Todd, J., Cartron, J.-P., Merriman, T., The molecular basis of the Kidd blood group polymorphism and its lack of association with type 1 diabetes susceptibility (1997) Hum. Mol. Genet., 6, pp. 1017-1020Chaudhuri, A., Polyakova, J., Zbrezezna, V., Williams, K., Gulati, S., Pogo, A.O., Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 10793-10797Iwamoto, S., Omi, T., Kajii, E., Ikemoto, S., Genomic organization of the glycophorin D gene: Duffy blood group Fy a/Fy b alloantigen system is associated with a polymorphism at the 44-amino residue (1995) Blood, 85, pp. 622-626Tournamille, C., Collin, Y., Cartron, J.-P., Van Le Kim, C., Disruption of a GATA motif in the Duffy gene promotor abolishes erythroid gene expression in Duffy-negative individuals (1995) Nature Genet., 10, pp. 224-228Rios, M., Reid, M.E., Naime, D., Chaudhuri, A., Pogo, A.O., Bianco, C., Importance of GATA box analysis in genotyping for the Duffy blood group system (1997) Transfusion, 37 (S), pp. 101S. , abstrZimmerman, P.A., Woolley, I., Masinde, G.L., Miller, S.M., McNamara, D.T., Hazlett, F., Mgone Alpers, M.P., Kazura, J.W., Emergence of FY*A(null) in a Plasmodium vivax-endemic region of Papua New Guinea (1999) Proc Natl Acad Sci. USA, 96 (24), pp. 13973-13977. , Nov 23Olsson, M.L., Hansson, C., Akesson, I.E., Avent, N.D., Daniels, G.L., Detection of the common alleles at the Duffy blood group locus by allele-specific primer PCR (1997) Transfusion, 37 (S), pp. 102S. , abstrCartron, J.-P., Bailly, P., Van Le Kim, C., Insights into the structure and function of membrane polypeptides carrying blood group antigens (1998) Vox Sang, 74 (SUPPL. 2), pp. 29-64Huang, C.H., Molecular insights into the Rh protein family and associated antigens (1997) Curr Opin Hematol., 4, pp. 94-103Huang, C.H., Blumenfeld, O.O., MNSs blood groups and major glycophorins: Molecular basis for allelic variation (1995) Molecular Basis of Major Human Blood Group Antigens, pp. 153-183. , cartron J-P, Pouger P, eds. New York: Plenum PressAvent, N.D., Reid, M.E., The Rh Blood group system: A review (2000) Blood, 95, pp. 1-1

The insulin receptor substrate 1 associates with phosphotyrosine phosphatase SHPTP2 in liver and muscle of rats

Insulin stimulates the tyrosine kinase activity of its receptor resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate-1 (IRS-1) which, in turn, associates with proteins containing SH2 domains. It has been shown that IRS-1 associates with the tyrosine phosphatase SHPTP2 in cell cultures. While the effect of the IRS-1/SHPTP2 association on insulin signal transduction is not completely known, this association may dephosphorylate IRS-1 and may play a critical role in the mitogenic actions of insulin. However, there is no physiological demonstration of this pathway of insulin action in animal tissues. In the present study we investigated the ability of insulin to induce association between IRS-1 and SHPTP2 in liver and muscle of intact rats, by co-immunoprecipitation with anti-IRS-1 antibody and anti-SHPTP2 antibody. In both tissues there was an increase in IRS-1 association with SHPTP2 after insulin stimulation. This association occurred when IRS-1 had the highest level of tyrosine phosphorylation and the decrease in this association was more rapid than the decrease in IRS-1 phosphorylation levels. The data provide evidence against the participation of SHPTP2 in IRS-1 dephosphorylation in rat tissues, and suggest that the insulin signal transduction pathway in rat tissues is related mainly to the mitogenic effects of the hormone

Educational Intervention For Self-care Of Individuals With Diabetes Mellitus [intervençã O Educativa Para O Autocuidado De Indíviduos Com Diabetes Mellitus]

Objective: To assess the knowledge, attitudes and practices of self-care in individuals with diabetes mellitus (DM) in a specialized health service in Belo Horizonte - MG Brazil Methods: The research was a case study with a qualitative approach. Data were collected by conducting focus groups with the participation of 12 individuals with diabetes and who attended three sessions of the educational program developed in this health service. Results: Data were analyzed using thematic analysis, which allowed the identification of the following categories: experiences; feelings; practical education for self-care associated with food and physical activity; perceived barriers to the pursuit of a healthy lifestyle; and expectations. Conclusion: The results of the study showed the importance of education and health communication guided the dialogical relations and appreciation of popular knowledge, by reorienting the educational practices for self care, in order to establish strategies for prevention and disease control.244514519Bandura, A., Self-efficacy: Toward a unifying theory of behavioral change (1977) Psychol Rev, 84 (2), pp. 191-215. , Comment in Percept Mot Skills. 2002;94(3 Pt 1): 1056Rosenstock, I.M., The health belief model: Explaining health behavior through expectancies (1990) Health Behavior and Health Education: Theory Research and Practice, pp. 39-62. , Glanz K Lewis FM Rimer BK editors. San Francisco: Jossey-Bass PublishersGatt, S., Sammut, R., An exploratory study of predictors of self-care behaviour in persons with type 2 diabetes (2008) Int J Nurs Stud, 45 (10), pp. 1525-33Freire, P., Pedagogia da autonomia: saberes necessários à (2002) Prática Educative, , 23a ed. São Paulo: Paz e TerraDiabetes, M., (2006) Brasília: Ministério da Saúde, p. 56. , Cadernos de Atenção Básica16)Torres, H.C., Franco, L.J., Stradioto, M.A., Hortale, V.A., Shall, V.T., Avaliação estratégica de educação em grupo e individual no programa educativo em diabetes (2009) Rev Saúde Pública =J Public Health, 43 (2), pp. 291-8Skinner, T.C., Carey, M.E., Cradock, S., Dallosso, H.M., Daly, H., Davies, M.J., Doherty, Y., Desmond, C., Educator talk' and patient change: Some insights from the DESMOND (Diabetes Education and Self Management for Ongoing and Newly Diagnosed) randomized controlled trial (2008) Diabet Med, 25 (9), pp. 1117-1120(2009) Ministério da Saúde. Estratégia Nacional Para A Educação em Saúde Para O Autocuidado em Diabetes Mellitus, p. 127. , Brasil, Florianópolis: SEAD/UFSCCruz Neto, O., Moreira, M.R., Sucena, L.F.M., (2001) Rfnet Grupos Focais e Pesquisa Social Qualitativa: O Debate Orientado Como Técnica de Investigação, , Rio de Janeiro: Ed FiocruzMinayo, M.C.S., (2004) O Desafio Do Conhecimento: Pesquisa Qualitativa em Saúde, , 8a ed. São Paulo: HucitecTabrizi, J.S., Wilson, A.J., Coyne, E.T., O'rourke, P.K., Clients' perspective on service quality for type 2 diabetes in Australia (2007) Aust N Z J Public Health, 31 (6), pp. 511-515Debono, M., Cachia, E., The impact of diabetes on psychological well being and quality of life. The role of patient education (2007) Psychology, Health and Medicine, 12 (5), pp. 545-555. , DOI 10.1080/13548500701235740, PII 781899164Funnell, M.M., Anderson, R.M., Empowerment and selfmanagement of diabetes (2004) Clin Diabetes, 22 (3), pp. 123-127Heisler, M., Piette, J.D., Spencer, M., Kieffer, E., Vijan, S., The relationship between knowledge of recent HbA1c values and diabetes care understanding and self-management (2005) Diabetes Care, 28 (4), pp. 816-822. , DOI 10.2337/diacare.28.4.816Siminerio, L.M., Funnell, M.M., Peyrot, M., Rubin, R.R., US nurses' perceptions of their role in diabetes care: Results of the cross-national Diabetes Attitudes Wishes and Needs (DAWN) study (2007) Diabetes Educ, 33 (1), pp. 152-162Shojania, K.G., Ranji, S.R., McDonald, K.M., Grimshaw, J.M., Sundaram, V., Rushakoff, R.J., Owens, D.K., Effects of quality improvement strategies for type 2 diabetes on glycemic control: A meta-regression analysis (2006) Journal of the American Medical Association, 296 (4), pp. 427-440. , http://jama.ama-assn.org/cgi/reprint/296/4/427, DOI 10.1001/jama.296.4.427Torres, H.C., Amaral, M.A., Amorim, M.M., Cyrino, A.P., Bodstein, R., Capacitação de profissionais da atenção primária à saúde para educação em Diabetes Mellitus (2010) Acta Paul Enferm, 23 (6), pp. 751-75

Translation And Adaptation Of The "diabetes Distress Scale - Dds" In Brazilian Culture [tradução E Adaptação Do "diabetes Distress Scale - Dds" Na Cultura Brasileira]

OBJECTIVE: To translate and adapt the Diabetes Distress Scale for the Brazilian culture. METHODS: The process followed international standards for adaptation of an instrument: translation, back translation and evaluation by a panel of judges, and pretest. RESULTS: The stages of translation and back translation were performed successfully, and the evaluation of the synthesis version by the expert panel resulted in modification of items, ensuring the equivalence between the original and translated versions. During the pretest, there were reformulations of some items, making them clearer and easier to understand. CONCLUSION: The Brazilian version of the Diabetes Distress Scale received satisfactory results with respect to the process of translation and cultural adaptation.255Moreira, R.O., Papelbaum, M., Appolinario, J.C., Matos, A.G., Coutinho, W.F., Meirelles, R.M., Diabetes mellitus and depression: A systematic review (2003) Arq Bras Endocrinol Metab, 47 (1), pp. 19-29Lustman, P.J., Anderson, R.J., Freedland, K.E., De Groot, M., Carney, R.M., Clouse, R.E., Depression and poor glycemic control: A meta-analytic review of the literature (2000) Diabetes Care, 23 (7), pp. 934-942Gonzalez, J.S., Safren, S.A., Cagliero, E., Wexler, D.J., Delahanty, L., Wittenberg, E., Depression, self-care, and medication adherence in type 2 diabetes: Relationships across the full range of symptom severity (2007) Diabetes Care, 30 (9), pp. 2222-2227Fisher, L., Mullan, J.T., Skaff, M.M., Glasgow, R.E., Arean, P., Hessler, D., Predicting diabetes distress in patients with type 2 diabetes: A longitudinal study (2009) Diabet Med, 26 (6), pp. 622-627Herschbach, P., Berg, P., Dankert, A., Duran, G., Engst-Hastreiter, U., Waadt, S., Fear of progression in chronic diseases: Psychometric properties of the fear of progression questionnaire (2005) J Psychosom Res, 58 (6), pp. 505-511Wexler, D.J., Grant, R.W., Wittenberg, E., Bosch, J.L., Cagliero, E., Delahanty, L., Correlates of health-related quality of life in type 2 diabetes (2006) Diabetologia, 49 (7), pp. 1489-1497Fisher, L., Glasgow, R.E., Mullan, J.T., Skaff, M.M., Polonsky, W.H., Development of a brief diabetes distress screening instrument (2008) Ann Fam Med, 6 (3), pp. 246-252Ting, R.Z., Nan, H., Yu, M.W., Kong, A.P., Ma, R.C., Wong, R.Y., Diabetes-related distress and physical and psychological health in chinese type 2 diabetic patients (2011) Diabetes Care, 34 (5), pp. 1094-1096Graue, M., Haugstvedt, A., Wentzel-Larsen, T., Iversen, M.M., Karlsen, B., Rokne, B., Diabetesrelated emotional distress in adults: Reliability and validity of the norwegian versions of the problem areas in diabetes scale (paid) and the diabetes distress scale (dds) (2012) Int J Nurs Stud, 49 (2), pp. 174-182Fisher, L., Glasgow, R.E., Strycker, L.A., The relationship between diabetes distress and clinical depression with glycemic control among patients with type 2 diabetes (2010) Diabetes Care, 33 (5), pp. 1034-1036Fisher, L., Mullan, J.T., Arean, P., Glasgow, R.E., Hessler, D., Masharani, U., Diabetes distress but not clinical depression or depressive symptoms is associated with glycemic control in both cross-sectional and longitudinal analyses (2010) Diabetes Care, 33 (1), pp. 23-28Polonsky, W.H., Fisher, L., Earles, J., Dudl, R.J., Lees, J., Mullan, J., Assessing psychosocial distress in diabetes: Development of the diabetes distress scale (2005) Diabetes Care, 28 (3), pp. 626-631Curcio, R., Lima, M.H., Alexandre, N.M., Diabetes mellitus related tools adapted and validated to Brazilian culture (2011) Rev Eletronica Enferm, 13 (2), pp. 331-337Global Guideline for Type 2 Diabetes: Recommendations for standard, comprehensive, and minimal care (2006) Diabet. Med, 23 (6), pp. 579-593. , IDF Clinical Guidelines Task ForceGuillemin, F., Cross-cultural adaptation and validation of health status measures (1995) Scand J Rheumatol, 24 (2), pp. 61-63Beaton, D.E., Bombardier, C., Guillemin, F., Ferraz, M.B., Guidelines for the process of crosscultural adaptation of self-report measures (2000) Spine. (Phila Pa 1976), 25 (24), pp. 3186-3191Alexandre, N.M., Guirardello, E.B., Cultural adaptation of instruments utilized in occupational health (2002) Rev Panam Salud Publica, 11 (2), pp. 109-111Wynd, C.A., Schmidt, B., Ma, S., Two quantitative approaches for estimating content validity (2003) West J Nurs Res, 25 (5), pp. 508-518El Achhab, Y., Nejjari, C., Chikri, M., Lyoussi, B., Disease-specific health-related quality of life instruments among adults diabetic: A systematic review (2008) Diabetes Res Clin Pract, 80 (2), pp. 171-184Torres, H.C., Souza, E.R., Lima, M.H., Bodstein, R.C., Educational intervention for self-care of individuals with diabetes mellitus (2011) Acta Paul Enferm, 24 (4), pp. 514-519. , Portugues