34 research outputs found
Separation of ammonium from industrial wastewater containing methanol by copper hexacyanoferrate (CuHCF) electrodes
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Anaerobic granulation for bioproduction : high rate production of medium chain carboxylic acids from thin stillage
Enrichment and characterisation of ethanol chain elongating communities from natural and engineered environments
Chain elongation is a microbial process in which an electron donor, such as ethanol, is used to elongate short chain carboxylic acids, such as acetic acid, to medium chain carboxylic acids. This metabolism has been extensively investigated, but the spread and differentiation of chain elongators in the environment remains unexplored. Here, chain elongating communities were enriched from several inocula (3 anaerobic digesters, 2 animal faeces and 1 caproic acid producing environment) using ethanol and acetic acid as substrates at pH 7 and 5.5. This approach showed that (i) the inoculumâs origin determines the pH where native chain elongators can grow; (ii) pH affects caproic acid production, with average caproic acid concentrations of 6.4â±â1.6â g·Lâ1 at pH 7, versus 2.3â±â1.8â g·Lâ1 at pH 5.5; however (iii) pH does not affect growth rates significantly; (iv) all communities contained a close relative of the known chain elongator Clostridium kluyveri; and (v) low pH selects for communities more enriched in this Clostridium kluyveri-relative (57.6â±â23.2% at pH 7, 96.9â±â1.2% at pH 5.5). These observations show that ethanol-consuming chain elongators can be found in several natural and engineered environments, but are not the same everywhere, emphasising the need for careful inoculum selection during process development
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Inhibitory Impact of Nitrite on the Anaerobic Ammonium Oxidizing (Anammox) Bacteria: Inhibition Mechanisms and Strategies to Improve the Reliability of the Anammox Process as a N-Removal Technology
The anaerobic oxidation of ammonium (anammox) with nitrite as electron acceptor is a microbial process that generates nitrogen gas as main final product. After being discovered in the Netherlands in the 1990s, anammox has been applied in state-of-the-art biotechnologies for the removal of N pollution from ammonium rich wastewaters. The anammox process offers significant advantages over traditional nitrification-denitrification based processes. Since anammox does not need elemental oxygen, it allows for important savings in aeration. Furthermore, due to the autotrophic nature of the bacteria, anammox does not require external addition of electron donor, often needed in systems with post-denitrification. Although the anammox bacteria have high specific activity, they are slow growing, with doubling times that can range from 10 to 25 d. Therefore, in case of a toxic event causing the death of the biomass, a long recovery period will be required to reestablish full treatment capacity. The purpose of this work is to investigate the inhibition of anammox bacteria by compounds commonly found in wastewaters, including substrates, intermediates and products of the anammox reaction. Among common wastewater constituents, sulfide was shown to be especially harmful, causing complete inhibition of anammox activity at concentrations as low as 11 mg HâS Lâ»Âč. Dissolved oxygen was moderately toxic with a 50% inhibiting concentration of 2.3 and 3.8 mg Lâ»Âč to granular and suspended anammox cultures, respectively. Among the various compounds involved in the anammox reaction, special attention was paid to nitrite. Numerous literature reports have indicated inhibition of anammox bacteria by its terminal electron acceptor. However to date, there is no consensus explanation as to the mechanism of nitrite inhibition nor on how the inhibition is impacted by variations in the physiological status of anammox cells. The mechanisms of anammox inhibition by nitrite were thoroughly investigated in batch and continuous experiments of this dissertation. The results of this work demonstrate that conditions hindering generation of metabolic energy have a detrimental effect on the tolerance of anammox cells to toxic levels of nitrite. The absence of ammonium during events of nitrite exposure was shown to exacerbate its toxic effect. As a result of nitrite inhibition, nitric oxide, an intermediate of the anammox reaction, accumulated in the head space of the batch experiments. Moreover, nitrite inhibition was enhanced at the lowest range of pH tested (6.4-7.2), while same nitrite concentrations caused no inhibition under mildly alkaline conditions (7.5-7.8). Although other authors have relied on the classic concept that undissociated nitrous acid is the species responsible for the inhibition, the results in this work indicate that the pH affects the inhibitory effect of nitrite, irrespective of the free nitrous acid concentration. Nitrite stress triggered an active response of the anammox bacteria, which temporarily increased their ATP content to mitigate the inhibition. Additionally, starvation of anammox microorganisms, caused during storage or by sustained underloading of bioreactors, was found to limit the capacity of the bacteria to tolerate exposure to nitrite. The results of this dissertation indicate that the tolerance of anammox bacteria to NOââ» inhibition relies on limiting its accumulation in sensitive regions of the cell. Active metabolism in presence of NHââș allows for active consumption of NOââ», avoiding accumulation of toxic intracellular NOââ» concentrations. Furthermore, secondary active transport proteins may be used by anammox bacteria to translocate nitrite to non-sensitive compartments. Nitrite active transport relies on a proton motive force. Therefore, conditions such as low pH (below 7.4) or absence of energy sources, which may disturb the maintenance of the intracellular proton gradient, will increase the sensitivity of anammox cells to NOââ» inhibition. Strategies for the operation and control of anammox bioreactors must be designed to avoid exposure of the biomass to nitrite under the absence of ammonium, low pH or after periods of starvation
Microbial electrosynthesis from CO2 : forever a promise?
Microbial electrosynthesis (MES) is an electrochemical process used to drive microbial metabolism for bio-production, such as the reduction of CO2 into industrially relevant organic products as an alternative to current fossil-fuel-derived commodities. After a decade of research on MES from CO2, figures of merit have increased significantly but are plateauing yet far from those expected to allow competitiveness for synthesis of commodity chemicals. Here we discuss the substantial technological shortcomings still associated with MES and evoke possible ways to mitigate them. It appears particularly challenging to obtain both relevant production rates (driven by high current densities) and energy conversion efficiency (i.e. low cell voltage) in microbial-compatible electrolytes. More competitive processes could arise by decoupling effective abiotic electroreductions (e.g. CO2 to CO or ethanol; H-2 evolution) with subsequent fermentation processes
An electrolytic hydrogen bubbling column reactor for high rate microbial electrosynthesis of acetate from CO2
Effetto dellâarricchimento in α-acido linolenico ed acido linoleico coniugato sulla stabilitĂ ossidativa di prodotti lattiero-caseari di diversa specie
L'obiettivo di questa ricerca Ăš stato quello di valutare l'ossidazione lipidica in diversi tipi di formaggi (pecorini e caprini), arricchiti in α-acido linolenico ed acido linoleico coniugato, al fine di accrescere il loro valore nutraceutico. Eâ stato adottato un regime alimentare animale arricchito in semi di lino estruso (capre: 180 g/capo/giorno; pecore: 240 g/capo/giorno) confrontato con una dieta di controllo. Lâeffetto di tale supplementazione sul contenuto in acidi grassi ω-3 ed in acido linoleico coniugato (CLA), Ăš risultato significativo nei formaggi trattati (pecorini: 2,6% ω-3, 2,9% CLA; caprini: 1,9% ω-3, 1,3% CLA), rispetto a quelli di controllo (pecorini: 0,9% ω-3, 1,1% CLA; caprini: 0,8% ω-3, 0,8% CLA). Il trattamento ha comportato un aumento significativo del livello di insaturazione dei formaggi (pecorini 46,8%; caprini 42,3%), rispetto a quelli di controllo (pecorini 32,6%; caprini 32,6%). La stagionatura non sembra aver influenzato significativamente la composizione in acidi grassi dei formaggi. Per valutare lo stato ossidativo dei formaggi, sono state eseguite determinazioni dei prodotti primari (numero di perossidi (POV)) e secondari dellâossidazione lipidica (sostanze reattive allâacido tiobarbiturico (TBARs) e prodotti di ossidazione del colesterolo (COPs)). La determinazione dei suddetti prodotti di ossidazione non ha evidenziato differenze significative, intra- ed interspecie, nĂ© tra i formaggi arricchiti e quelli di controllo nĂ© tra gli stessi freschi e stagionati. In generale, Ăš stato riscontrato un basso contenuto di POV (0,9-2,3 meq O2/Kg lipidi), di TBARs (nd-0,025 mg MDA/Kg formaggio) e di COPs (0,7-1,5 mg/100 g lipidi, corrispondente a 0,2-0,4% di colesterolo ossidato), per cui lâarricchimento in acidi grassi ω-3 non ha significativamente favorito lâossidazione della frazione lipidica dei diversi formaggi, non destando preoccupazione per la salute del consumatore