Effects of different operating conditions on sediment slurry sequencing batch reactors treating marine port sediments contaminated by PAHs

Due to intensive anthropic activities, marine port sediments are often contaminated by organic pollutants like polycyclic aromatic hydrocarbons (PAHs), which represent a significant environmental threat. In this study, two sediment slurry sequencing batch reactors (SS-SBRs) were used to biologically degrade a mixture of PAHs (namely fluorene, phenanthrene, fluoranthene and pyrene) from marine sediments dredged from Cagliari (Italy) and El Kantaoui (Tunisia) ports. To enhance PAHs removal by biostimulation, nutrients were added in both sediments, acetate was used as co-substrate (El Kantaoui, SS-SBR1), and saponins were used as surfactants (Cagliari, SS-SBR2). Moreover, different levels of contamination (up to 200 mgPAHtot/kgdw in SS-SBR1; up to 400 mgPAHtot/kgdw in SS-SBR2) and solid to liquid ratios (S/L, up to 0.2 in SS-SBR1; up to 0.1 in SS-SBR2) were applied. As to SS-SBR1, the highest removal efficiencies (99-100% and 98-100% for 3- and 4-ring PAHs, respectively) were achieved when the level of contamination and S/L were set at 200 mgPAH-tot/kgdw and 0.1, 150 mgPAH-tot/kgdw and 0.13, and 150 mgPAH-tot/kgdw and 0.20, respectively; as to SS-SBR2, very high removal efficiencies (99÷100% and 98÷100% for 3- and 4-ring PAHs, respectively) were observed during the whole experimental campaign. Such results are promising and provide a useful background for testing other important operating parameters (e.g., the volumetric exchange ratio)

Clinical characteristics, neuroimaging findings, and neuropsychological functioning in attention-deficit hyperactivity disorder: Sex differences

Recent clinical studies, in both children/adolescents and adults, have shown the extreme neuropsychological heterogeneity of attention-deficit hyperactivity disorder (ADHD): specific neuropsychological deficits have been found only in a minority of individuals, with no direct correlation between discrete cognitive performances and the trajectory of clinical symptoms. Deficits in specific neuropsychological functions may be common in ADHD, but nevertheless no cognitive or neuropsychological profile may fully explain the disorder. Sex differences in the ADHD presentation, both at a neuropsychological and clinical level, also contribute to this clinical and neuropsychological heterogeneity. At a neuropsychological level, females with ADHD may show greater working memory problems, poorer vocabulary skills and worse visual spatial reasoning. Structural and functional imaging study also show discrete differences across sex; however, the great majority of clinical studies mainly or exclusively include male participants with insufficient data to draw firm conclusions on sex differences within the disorder. Here, we report the recent literature data, discussing still open research questions about the clinical presentation, neuroimaging findings, and neuropsychological functioning in ADHD with a focus on the impact of sex differences—a deeper insight in these unresolved issues may have relevant clinical and therapeutic implications for tailored, effective, and long-lasting interventions

Adsorption and release of sulfamethizole from mesoporous silica nanoparticles functionalised with triethylenetetramine

Mesoporous silica nanoparticles (MSN) were synthesised and functionalised with tri-ethylenetetramine (MSN-TETA). The samples were fully characterised (transmission electron mi-croscopy, small angle X-ray scattering, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential and nitrogen adsorption/desorption isotherms) and used as carriers for the adsorption of the antimicrobial drug sulphamethizole (SMZ). SMZ loading, quantified by UV–Vis spectroscopy, was higher on MSN-TETA (345.8 mg g−1) compared with bare MSN (215.4 mg g−1) even in the presence of a lower surface area (671 vs. 942 m2 g−1). The kinetics of SMZ adsorption on MSN and MSN-TETA followed a pseudo-second-order model. The adsorption isotherm is described better by a Langmuir model rather than a Temkin or Freundlich model. Release kinetics showed a burst release of SMZ from bare MSN samples (k1 = 136 h−1) in contrast to a slower release found with MSN-TETA (k1 = 3.04 h−1), suggesting attractive intermolecular interactions slow down SMZ release from MSN-TETA. In summary, the MSN surface area did not influence SMZ adsorption and release. On the contrary, the design of an effective drug delivery system must consider the intermolecular interactions between the adsorbent and the adsorbate

Efficient Nitrogen Recovery from Agro-Energy Effluents for Cyanobacteria Cultivation (Spirulina)

The present study aimed to obtain an efficient liquid nitrogen fertilizer from the by-product of anaerobic digestion for its subsequent use in the production of cyanobacteria (Spirulina). A simple recovery technology was tested based on the stripping and acid absorption, modifying temperature (50 and 70 degrees C) and pH (10 and 12), of the ammonia nitrogen contained in the digestate produced in a large-scale plant treating livestock manure and grass silage. The results demonstrated how, at a relatively low temperature (50 degrees C), using sulfuric and citric acid solution, it is possible to recover nitrogen from a digestate in the form of ammonium sulfate and ammonium citrate with yields of 70% and 72.1% respectively. By carrying out Spirulina growth tests, promising results were obtained under semicontinuous production, with a maximum dry biomass daily productivity of 0.344 g L-1 day(-1) with ammonium sulfate and 0.246 gDW L-1 day(-1) with ammonium citrate. The results showed that nitrogen can be efficiently recovered on site by using the organic acid, digestate and waste heat from anaerobic digestion for Spirulina biomass production

Diagnostic therapeutic care pathway for pediatric food allergies and intolerances in Italy: a joint position paper by the Italian Society for Pediatric Gastroenterology Hepatology and Nutrition (SIGENP) and the Italian Society for Pediatric Allergy and Immunology (SIAIP)

Epidemiologic data suggest an increased prevalence of pediatric food allergies and intolerances (FAIs) during the last decades. This changing scenario has led to an increase in the overall healthcare costs, due to a growing demand for diagnostic and treatment services. There is the need to establish Evidence-based practices for diagnostic and thera- peutic intervention that could be adopted in the context of public health policies for FAIs are needed. This joint position paper has been prepared by a group of experts in pediatric gastroenterology, allergy and nutrition from the Italian Society for Pediatric Gastroenterology Hepatology and Nutrition (SIGENP) and the Italian Society for Pediatric Allergy and Immunology (SIAIP). The paper is focused on the Diagnostic Therapeutic Care Pathway (DTCP) for pediatric FAIs in Italy

β-N-acetylglucosaminidase grafted on mesoporous silica nanoparticles. A bionanoantibiotic system against Staphylococcus aureus bacteria

A bionanoantibiotic system based on beta-N-acetylglucosaminidase (Ami) and Lysozyme (Lyz) enzymes grafted on the external surface of amino functionalized mesoporous silica nanoparticles, having a radial arrangement of pores (MSNr-NH2), was prepared and fully characterized. Before the enzyme grafting the nanoparticles were also loaded with the antibiotic drug levofloxacin (Levo) to explore the possible synergic effect with the enzymes. MSNr-NH2-Lyz-Levo and MSNr-NH2-Ami-Levo did not show any activity against S. aureus. On the contrary, in the absence of the antibiotic, both Lyz and Ami immobilized on MSNr were able to destroy S. aureus cells, suggesting an inhibiting action of the antibiotic on the enzymes. Although the loading of immobilized Lyz was higher than that of Ami (76 vs. 20 mg/g, respectively), the highest antibacterial efficacy was found for MSNr-NH2-Ami nanoantibiotic. Moreover, MSNr-NH2-Ami was active against S. aureus even at very low concentration (12.5 mu g/ mL) with a bactericidal activity (79%), higher than that determined for MSNr-NH2 loaded with levofloxacin (54%). These results suggest the possibility of using enzyme grafted MSNr as a bionanoantibiotic drug with high efficiency even at low nanoparticles concentration

Neuropsychological characterization of aggressive behavior in children and adolescents with cd/odd and effects of single doses of medications: The protocol of the matrics_wp6-1 study

Aggressive behaviors and disruptive/conduct disorders are some of the commonest reasons for referral to youth mental health services; nevertheless, the efficacy of therapeutic interventions in real-world clinical practice remains unclear. In order to define more appropriate targets for innovative pharmacological therapies for disruptive/conduct disorders, the European Commission within the Seventh Framework Programme (FP7) funded the MATRICS project (Multidisciplinary Approaches to Translational Research in Conduct Syndromes) to identify neural, genetic, and molecular factors underpinning the pathogenesis of aggression/antisocial behavior in preclinical models and clinical samples. Within the program, a multicentre case-control study, followed by a single-blind, placebo-controlled, cross-over, randomized acute single-dose medication challenge, was conducted at two Italian sites. Aggressive children and adolescents with conduct disorder (CD) or oppositional defiant disorder (ODD) were compared to the same age (10–17 y) typically developing controls (TDC) on a neuropsychological tasks battery that included both “cold” (e.g., inhibitory control, decision making) and “hot” executive functions (e.g., moral judgment, emotion processing, risk assessment). Selected autonomic measures (heart rate variability, skin conductance, salivary cortisol) were recorded before/during/after neuropsychological testing sessions. The acute response to different drugs (methylphenidate/atomoxetine, risperidone/aripiprazole, or placebo) was also examined in the ODD/CD cohort in order to identify potential neuropsychological/physiological mechanisms underlying aggression. The paper describes the protocol of the clinical MATRICS WP6-1 study, its rationale, the specific outcome measures, and their implications for a precision medicine approach

Tolerogenic Effect Elicited by Protein Fraction Derived From Different Formulas for Dietary Treatment of Cow’s Milk Allergy in Human Cells

Several formulas are available for the dietary treatment of cow’s milk allergy (CMA). Clinical data suggest potentially different effect on immune tolerance elicited by these formulas. We aimed to comparatively evaluate the tolerogenic effect elicited by the protein fraction of different formulas available for the dietary treatment of CMA. Five formulas were compared: extensively hydrolyzed whey formula (EHWF), extensively hydrolyzed casein formula (EHCF), hydrolyzed rice formula (HRF), soy formula (SF), and amino acid-based formula (AAF). The formulas were reconstituted in water according to the manufacturer’s instructions and subjected to an in vitro infant gut simulated digestion using a sequential gastric and duodenal static model. Protein fraction was then purified and used for the experiments on non-immune and immune components of tolerance network in human enterocytes and in peripheral mononuclear blood cells (PBMCs). We assessed epithelial layer permeability and tight junction proteins (occludin and zonula occludens-1, ZO-1), mucin 5AC, IL-33, and thymic stromal lymphopoietin (TSLP) in human enterocytes. In addition, Th1/Th2 cytokine response and Tregs activation were investigated in PBMCs from IgE-mediated CMA infants. EHCF-derived protein fraction positively modulated the expression of gut barrier components (mucin 5AC, occludin and ZO-1) in human enterocytes, while SF was able to stimulate the expression of occludin only. EHWF and HRF protein fractions elicited a significant increase in TSLP production, while IL-33 release was significantly increased by HRF and SF protein fractions in human enterocytes. Only EHCF-derived protein fraction elicited an increase of the tolerogenic cytokines production (IL-10, IFN-γ) and of activated CD4+FoxP3+ Treg number, through NFAT, AP1, and Nf-Kb1 pathway. The effect paralleled with an up-regulation of FoxP3 demethylation rate. Protein fraction from all the study formulas was unable to induce Th2 cytokines production. The results suggest a different regulatory action on tolerogenic mechanisms elicited by protein fraction from different formulas commonly used for CMA management. EHCF-derived protein fraction was able to elicit tolerogenic effect through at least in part an epigenetic modulation of FoxP3 gene. These results could explain the different clinical effects observed on immune tolerance acquisition in CMA patients and on allergy prevention in children at risk for atopy observed using EHCF