An Outer Membrane Vesicle-Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability

When searching for new antibiotics against Gram-negative bacterial infections, a better understanding of the permeability across the cell envelope and tools to discriminate high from low bacterial bioavailability compounds are urgently needed. Inspired by the phospholipid vesicle-based permeation assay (PVPA), which is designed to predict non-facilitated permeation across phospholipid membranes, outer membrane vesicles (OMVs) of Escherichia coli either enriched or deficient of porins are employed to coat filter supports for predicting drug uptake across the complex cell envelope. OMVs and the obtained in vitro model are structurally and functionally characterized using cryo-TEM, SEM, CLSM, SAXS, and light scattering techniques. In vitro permeability, obtained from the membrane model for a set of nine antibiotics, correlates with reported in bacterio accumulation data and allows to discriminate high from low accumulating antibiotics. In contrast, the correlation of the same data set generated by liposome-based comparator membranes is poor. This better correlation of the OMV-derived membranes points to the importance of hydrophilic membrane components, such as lipopolysaccharides and porins, since those features are lacking in liposomal comparator membranes. This approach can offer in the future a high throughput screening tool with high predictive capacity or can help to identify compound- and bacteria-specific passive uptake pathways

Flux noise in high-temperature superconductors

Spontaneously created vortex-antivortex pairs are the predominant source of flux noise in high-temperature superconductors. In principle, flux noise measurements allow to check theoretical predictions for both the distribution of vortex-pair sizes and for the vortex diffusivity. In this paper the flux-noise power spectrum is calculated for the highly anisotropic high-temperature superconductor Bi-2212, both for bulk crystals and for ultra-thin films. The spectrum is basically given by the Fourier transform of the temporal magnetic-field correlation function. We start from a Berezinskii-Kosterlitz-Thouless type theory and incorporate vortex diffusion, intra-pair vortex interaction, and annihilation of pairs by means of a Fokker-Planck equation to determine the noise spectrum below and above the superconducting transition temperature. We find white noise at low frequencies omega and a spectrum proportional to 1/omega^(3/2) at high frequencies. The cross-over frequency between these regimes strongly depends on temperature. The results are compared with earlier results of computer simulations.Comment: 9 pages, 4 PostScript figures, to be published in Phys. Rev.

Entangled state preparation via dissipation-assisted adiabatic passages

The main obstacle for coherent control of open quantum systems is decoherence due to different dissipation channels and the inability to precisely control experimental parameters. To overcome these problems we propose to use dissipation-assisted adiabatic passages. These are relatively fast processes where the presence of spontaneous decay rates corrects for errors due to non-adiabaticity while the system remains in a decoherence-free state and behaves as predicted for an adiabatic passage. As a concrete example we present a scheme to entangle atoms by moving them in and out of an optical cavity.Comment: 11 pages, 7 figures, minor changes, accepted for publication in Phys. Rev.

Lattice Pseudospin Model for ν=1\nu=1 Quantum Hall Bilayers

We present a new theoretical approach to the study of $\nu=1$ quantum Hall bilayer that is based on a systematic mapping of the microscopic Hamiltonian to an anisotropic SU(4) spin model on a lattice. To study the properties of this model we generalize the Heisenberg model Schwinger boson mean field theory (SBMFT) of Arovas and Auerbach to spin models with anisotropy. We calculate the temperature dependence of experimentally observable quantities, including the spin magnetization, and the differential interlayer capacitance. Our theory represents a substantial improvement over the conventional Hartree-Fock picture which neglects quantum and thermal fluctuations, and has advantages over long-wavelength effective models that fail to capture important microscopic physics at all realistic layer separations. The formalism we develop can be generalized to treat quantum Hall bilayers at filling factor $\nu=2$.Comment: 26 pages, 10 figures. The final version, to appear in PR

The status of GEO 600

The GEO 600 laser interferometer with 600m armlength is part of a worldwide network of gravitational wave detectors. GEO 600 is unique in having advanced multiple pendulum suspensions with a monolithic last stage and in employing a signal recycled optical design. This paper describes the recent commissioning of the interferometer and its operation in signal recycled mode

Skymrion lattice melting in the quantum Hall system

The melting and magnetic disordering of the skyrmion lattice in the quantum Hall system at filling factor $\nu\approx 1$ are studied. A Berezinskii-Kosterlitz-Thouless renormalization group theory is employed to describe the coupled magnetic and translational degrees of freedom. The non-trivial magnetic properties of the skyrmion system stem from the in-plane components of the non-collinear magnetization in the vicinity of skyrmions, which are described by an antiferromagnetic XY model. In a Coulomb gas formulation the `particles' are the topological defects of the XY model (vortices) and of the lattice (dislocations and disclinations). The latter frustrate the antiferromagnetic order and acquire fractional vorticity in order to minimize their energy. We find a number of melting/disordering scenarios for various lattice types. While these results do not depend on a particular model, we also consider a simple classical model for the skyrmion system. It results in a rich T=0 phase diagram. We propose that the triangular and square skyrmion lattices are generically separated by a centered rectangular phase in the quantum Hall system.Comment: 15 pages with 5 figures. Minor revisions. Important reference to M. Rao, S. Sengupta, and R. Shankar, Phys. Rev. Lett. 79, 3998 (1997) adde

Meta-analysis identifies seven susceptibility loci involved in the atopic March

Eczema often precedes the development of asthma in a disease course called the a 'atopic march'. To unravel the genes underlying this characteristic pattern of allergic disease, we conduct a multi-stage genome-wide association study on infantile eczema followed by childhood asthma in 12 populations including 2,428 cases and 17,034 controls. Here we report two novel loci specific for the combined eczema plus asthma phenotype, which are associated with allergic disease for the first time; rs9357733 located in EFHC1 on chromosome 6p12.3 (OR 1.27; P=2.1 × 10 a'8) and rs993226 between TMTC2 and SLC6A15 on chromosome 12q21.3 (OR 1.58; P=5.3 × 10 a'9). Additional susceptibility loci identified

Meta-analysis of Genome-Wide Association Studies for Extraversion: Findings from the Genetics of Personality Consortium

Extraversion is a relatively stable and heritable personality trait associated with numerous psychosocial, lifestyle and health outcomes. Despite its substantial heritability, no genetic variants have been detected in previous genome-wide association (GWA) studies, which may be due to relatively small sample sizes of those studies. Here, we report on a large meta-analysis of GWA studies for extraversion in 63,030 subjects in 29 cohorts. Extraversion item data from multiple personality inventories were harmonized across inventories and cohorts. No genome-wide significant associations were found at the single nucleotide polymorphism (SNP) level but there was one significant hit at the gene level for a long non-coding RNA site (LOC101928162). Genome-wide complex trait analysis in two large cohorts showed that the additive variance explained by common SNPs was not significantly different from zero, but polygenic risk scores, weighted using linkage information, significantly predicted extraversion scores in an independent cohort. These results show that extraversion is a highly polygenic personality trait, with an architecture possibly different from other complex human traits, including other personality traits. Future studies are required to further determine which genetic variants, by what modes of gene action, constitute the heritable nature of extraversion

ARIA 2016: Care pathways implementing emerging technologies for predictive medicine in rhinitis and asthma across the life cycle

The Allergic Rhinitis and its Impact on Asthma (ARIA) initiative commenced during a World Health Organization workshop in 1999. The initial goals were (1) to propose a new allergic rhinitis classification, (2) to promote the concept of multi-morbidity in asthma a