Optimization of microwave-assisted extraction of phenolic compounds with antioxidant activity from carob pods

[EN] A microwave-assisted extraction (MAE) procedure to obtain phenolic compounds from carob bark was optimized by using response surface methodology. A four-factor, three-level Box-Behnken design with five central points was used to evaluate the influence of temperature, solid-liquid ratio, ethanol concentration and time in carob bark extraction in terms of antioxidant activity (DPPH) and total extraction yield. Optimal extraction conditions were found using 80 ºC, 35% (v/v) ethanol, a ratio of 35 mL/g and 29.5 min. Total phenolics content (TPC), antioxidant activity (DPPH, FRAP, ABTS), carbohydrates content and main polyphenols composition (HPLC) were determined at optimal conditions. An experimental total yield of 66.5% was obtained with a TPC value of 33.6 mg GAE/g DW and polysaccharides content of 345.4 mg glucose/g DW. A high antioxidant activity was also shown by the three methods tested. The results showed the potential of carob pods skin as a natural source of phenolic compounds, in particular gallic acid, and the effectiveness of MAE as extraction technique for the revalorization of this agro-food waste.This research was supported by the Spanish Ministry of Science, Innovation and Universities (MICIU) programs MAT2017-84909-C2-2-R and AGL2015-63855-C2-1-R and by the EU H2020 project YPACK (reference number 773872). Quiles-Carrillo wants to thank GV for his FPI grant (ACIF/2016/182) and MECD for his FPU grant (FPU15/03812). Torres-Giner also thank MINECO for his Juan de la Cierva contract (IJCI-2016-29675).Quiles-Carrillo, L.; Mellinas, C.; Garrigos, M.; Balart, R.; Torres-Giner, S. (2019). Optimization of microwave-assisted extraction of phenolic compounds with antioxidant activity from carob pods. 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