Solute-solvent distribution function (gss) for specific residues.

<p>(A) charged glutamate residue; (B) polar residues and (C) non-polar residues. Non-polar interactions with hydrophobic residues are highlighted in purple. Several interactions with peptide backbone were identified.</p

Total phenolic and flavonoid content of <i>Campomanesia adamantium</i> peel extract.

<p>Total phenolic and flavonoid content of <i>Campomanesia adamantium</i> peel extract.</p

Effect of <i>Campomanesia adamantium</i> peel extract on cGMP levels in T84 cells.

<p>Cells were pretreated with extract (0.25, 0.5, 1, 2.5, 5 and 10 mg/mL) or vehicle (DPBS) for 10 min and then treated with 1 μM of STa for 10 min. STa was added in groups of <i>C</i>. <i>adamantium</i> (all concentrations), Tadalafil e FPIPP. TAD (tadalafil) and FPIPP (antidiarrheal drug) was used as control drugs that increase and decrease cGMP levels, respectively. Results are presented as mean + SEM. n = 4–6; One-way ANOVA followed by Tukey´s test; #P <0.05 compared to baseline group; *P <0.05 compared to STa group.</p

Effect of <i>Campomanesia adamantium</i> peel extract on cell metabolic activity.

<p>T84 cells were treated with 10 mg/mL of extract or vehicle (medium) for 1h, 24h and 48h. Results are presented as mean + SEM. n = 9; Two-way ANOVA followed by Tukey´s test; *P <0.05 compared to vehicle group.</p

Solute-solvent distribution function (gss) for all residues and water (cyan) or gallic acid (red).

<p>Molecular view at three stages of simulation, initial, 50 ns and last frame, showing the increase of phenol concentration at ~1.7 Å and ~2.5 Å for all peptide. The H-bonds were calculated for water and gallic acid with STa.</p

Effect of the APMO in exposed rats as measured by the comet assay.

<p>The groups were treated with 125, 250, 500 and 1000 mg.kg^-1 of the <i>Attalea phalerata</i> Mart. ex Spreng. oil (APMO) daily for 28 consecutive days (four weeks) and the negative control group received the vehicle (saline + Tween 80^®). The positive control group received intraperitoneal injection of cyclophosphamide at 20 mg.kg^-1.</p

Characterization of the α-carotene and β-carotene values by chromatographic analysis in <i>Attalea phalerata</i> Mart. ex Spreng. oil (APMO).

<p>Characterization of the α-carotene and β-carotene values by chromatographic analysis in <i>Attalea phalerata</i> Mart. ex Spreng. oil (APMO).</p

Effects of the APMO on cell metabolic activity by MTT assay.

<p>T84 cells were treated with 1, 2.5, 5, 10 and 1 mg.mL^-1 of oil or vehicle (medium) for 1h, 24h and 48h. Results are presented as mean + SEM. n = 8; One-way ANOVA followed by Tukey´s test; *p<0.05 compared to vehicle (medium) and experimental group (1, 2.5, 5, 10 and 1 mg.mL^-1 of oil).</p

Effects of the APMO on <i>Artemia salina</i> assay.

<p><i>Artemia salina</i> nauplii were treated with 10, 50, 100, 250 and 500 μg.mL^-1 of oil or vehicle (medium) for 24 h. Results are presented as mean + SEM. n = 4; One-way ANOVA followed by Tukey´s test; *p<0.05 compared to vehicle (medium) and experimental group (10, 50, 100, 250 and 500 μg.mL^-1 of oil).</p

Effect of the <i>A</i>. <i>phalerata</i> oil on the leukocyte migration induced by carrageenan in the pleurisy model in rats.

<p>Values expressed in mean ± standard error of the mean. n = 5. * P <0.05 (ANOVA / Tukey) compared with the negative control (-).</p