287 research outputs found

    Caspofungin Treatment of Aspergillus fumigatus Results in ChsG-Dependent Upregulation of Chitin Synthesis and the Formation of Chitin-Rich Microcolonies

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    Date of Acceptance: 23/07/2015 We thank Gillian Milne for help with electron microscopy, Sophie M. Schäfer for pilot experiments, and Emilia Mellado for strains. All authors acknowledge financial support of Gilead Sciences through Ph.D. studentships for L.A.W. and K.K.L. We also acknowledge research grants from the Wellcome Trust (080088, 086827, 075470, 099215, and 097377) and the British Society for Antimicrobial Chemotherapy.Peer reviewedPublisher PD

    Caspofungin induced cell wall changes of Candida species influences macrophage interactions

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    Acknowledgements We thank Gillian Milne from the University of Aberdeen Microscopy and Histology facility for help with EM, Dr David Stead (Aberdeen Proteomics) for proteomics analysis and Prof. Gordon Brown for Fc:Dectin1. We thank Dr Judith Bain and Prof. Lars Erwig for advice on the macrophage assays. Funding We acknowledge funding from the British Society for Antimicrobial Chemotherapy and the Medical Research Council (G0400284).Peer reviewedPublisher PD

    Fungal Cell Wall Proteins and Signaling Pathways Form a Cytoprotective Network to Combat Stresses

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    Funding: This research was funded by Abia State University and the University of Aberdeen.Peer reviewedPublisher PD

    Dissection of the Candida albicans class I chitin synthase promoters

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    We acknowledge financial support from the Biotechnology and Biological Sciences Research Council (10161), Medical Research Council (New Investigator Award to C.A.M.), the European Community FUNGALWALL and SIGNALPATH initiatives and the Wellcome Trust.Peer reviewedPublisher PD

    Sfp1 and Rtg3 reciprocally modulate carbon source-conditional stress adaptation in the pathogenic yeast Candida albicans

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    Acknowledgements We thank Aaron Mitchell, Dominique Sanglard and Suzanne Noble for their generosity in providing mutant collections, and Linghuo Jiang for generously providing strains. We also thank Susan Budge for her support and excellent technical assistance. We also thank the qPCR Facility in the Institute of Medical Sciences, and particularly Fiona Saunders for her great advice and help. SLK was supported by a PhD scholarship from the University of Aberdeen. AJPB was supported by the UK Biotechnology and Biological Research Council (BB/F00513X/1; BB/K017365/1), by the European Research Council (STRIFE Advanced Grant; ERC-2009-AdG-249793), and by the UK Medical Research Council (MR/M026663/1). AJPB and CAM were also supported by the Wellcome Trust (088858; 097377), and by the MRC Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1).Peer reviewedPublisher PD

    Host responses in an ex-vivo human skin model challenged with Malassezia sympodialis

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    FUNDING: This project was funded by a Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology (097377/Z/11/Z). We would like to acknowledge the support of Internal Funding through a Core Facilities Voucher from the University of Aberdeen. ACKNOWLEDGMENTS: The authors gratefully acknowledge the Technology hubs at the University of Aberdeen (Microscopy and Histology, qPCR facility and Proteomics) for their support, sample processing and training. Special thanks to Professor Annika Scheynius from the Karolinska Institute, Stockholm, Sweden for sharing her expertise and constructive discussions and for giving us the inspiration to work on Malassezia.Peer reviewedPublisher PD

    A High-Throughput Candida albicans Two-Hybrid System

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    We thank Nico Vangoethem for help with preparation of the figures and Ilse Palmans, Tom Adriany, and Selien Schots for technical assistance. Financial support was obtained from the Interuniversity Attraction Poles Programme initiated by the Belgian Science Policy Office (IAP P7/28) and by the KU Leuven Research Council (C14/17/063). C.D. acknowledges support from the French Government’s Investissement d’Avenir program (Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases, ANR-10-LABX-62-IBEID). C.A.M. and C.D. acknowledge support from the Wellcome Trust (088858/Z/09/Z). C.A.M. acknowledges support from the MRC Centre for Medical Mycology (MR/N006364/1) and the University of Aberdeen.Peer reviewedPublisher PD
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