Ocimum gratissimum Linn. and rosmarinic acid, attenuate eosinophilic airway inflammation in an experimental model of respiratory allergy to Blomia tropicalis.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-08-25T19:10:59Z No. of bitstreams: 1 Costa RS Ocimum gratissimum Linn ........pdf: 2216829 bytes, checksum: 513639b3e092aea34adaa12835404c4a (MD5)Made available in DSpace on 2014-08-25T19:10:59Z (GMT). No. of bitstreams: 1 Costa RS Ocimum gratissimum Linn ........pdf: 2216829 bytes, checksum: 513639b3e092aea34adaa12835404c4a (MD5) Previous issue date: 2012Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilUniversidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. Paraíba, JP, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilAllergic asthma has emerged as an important public health problem of urban populations in developed countries. Very often herbal medicine is used to treat this widespread disease, due to the lack of efficacy and the important side effects related to the classical drugs in use. Along this line, Ocimum gratissimum (Og) is a plant widely used in Brazilian folk medicine to treat inflammatory disorders, such as asthma. In the present study we evaluated the immunomodulatory effects of Og and rosmarinic acid (RA, a polyphenolic compound) in a murine model of respiratory allergy induced by the Blomia tropicalis (Bt) mite. The respiratory allergy was induced in A/J mice by administration of Bt extract and the treatment was done using 25, 50 or 100mg/kg of an Og methanolic extract or using 2, 20 or 200mg/kg of RA. We then evaluated the changes induced by these drugs on immunological parameters related to the allergic process, which are up-regulated in this allergic model. The treatment of animals with 100mg/Kg Og and 200mg/Kg RA led to a significant reduction in the numbers of leukocytes/eosinophils in bronchoalveolar lavage (BAL); eosinophil peroxidase activity in BAL; presence of mucus in respiratory tract, histopathological changes in the lung, and IL-4 in BAL. These results suggest that the methanolic extract of Og and the polyphenol RA have therapeutic potential in this murine model of respiratory allergy to a clinically relevant human sensitizer allergen

Polymorphisms in the DAD1 and OXA1L genes are associated with asthma and atopy in a South American population

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-16T11:49:50Z No. of bitstreams: 1 Pires AO Polymorphisms in the DAD1 and OXA1L....pdf: 1226142 bytes, checksum: c0fa5af323611b005d0a4abe202c2c33 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-16T11:59:43Z (GMT) No. of bitstreams: 1 Pires AO Polymorphisms in the DAD1 and OXA1L....pdf: 1226142 bytes, checksum: c0fa5af323611b005d0a4abe202c2c33 (MD5)Made available in DSpace on 2018-08-16T11:59:43Z (GMT). No. of bitstreams: 1 Pires AO Polymorphisms in the DAD1 and OXA1L....pdf: 1226142 bytes, checksum: c0fa5af323611b005d0a4abe202c2c33 (MD5) Previous issue date: 2018Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo a Pesquisa do Estado da Bahia (FAPESB).Federal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Allergy and Acarology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilAtopic asthma, which is characterized by the chronic inflammation and morbidity of airways, is a disease of great complexity, and multiple genetic and environmental factors are involved in its etiology. In the first genome-wide association study (GWAS) conducted in Brazil for asthma, a positive association was found between atopic asthma and a variant (rs1999071), which is located between the DAD1 and OXA1L genes, although neither gene has previously been reported to be associated with asthma or allergies. The DAD1 gene is involved in the regulation of programmed cell death, and OXA1L is involved in biogenesis and mitochondrial oxidative phosphorylation. This study aimed to evaluate how polymorphisms in DAD1 and OXA1L are associated with asthma and markers of atopy in individuals from the Salvador cohort of the SCAALA (Social Change Asthma and Allergy in Latin America) program. The DNA of 1220 individuals was genotyped using the Illumina 2.5 Human Omni Bead chip. Logistic regression analyses were performed with PLINK 1.9 software to verify the association between DAD1 and OXA1L polymorphisms and asthma and atopic markers, adjusted for sex, age, helminth infections and ancestry markers, using an additive model. The DAD1 and OXA1L genes were associated with some of the evaluated phenotypes, such as asthma, skin prick test (SPT), specific IgE for aeroallergens, and Th1/Th2-type cytokine production. Using qPCR, as well as in silico gene expression analysis, we have demonstrated that some of the polymorphisms in both genes are able to affect their respective gene expression levels. In addition, DAD1 was over-expressed in asthmatic patients when compared with controls. Thus, our findings demonstrate that variants in both the DAD1 and OXA1L genes may affect atopy and asthma in a Latin American population with a high prevalence of asthma