Detection of Panulirus argus Virus 1 (PaV1) in the Caribbean spiny lobster using fluorescence in situ hybridization (FISH)

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and 10 ng of genomic DNA extracted from the hemolymph of diseased spiny lobster. The fluorescein (FITC)-labeled probe specifically hybridized to PaV1-infected cells in the hepatopancreas, hindgut, gills, heart, foregut, and nerve tissues. FITC staining was observed around the inner periphery of the nuclear membrane, with lighter staining in a more dispersed pattern within the nucleus. The probe did not hybridize with host tissues of uninfected spiny lobsters, nor did it cross-react with 4 other virus samples tested. This assay will facilitate our understanding of the pathogenesis of the viral disease and help in monitoring efforts directed at determining the prevalence of PaV1 in juvenile nurseries for this lobster

Detection of Panulirus Argus Virus 1 (PaV1) in the Caribbean Spiny Lobster Using Fluorescence in situ Hybridization (FISH)

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and 10 ng of genomic DNA extracted from the hemolymph of diseased spiny lobster. The fluorescein (FITC)-labeled probe specifically hybridized to PaV1-infected cells in the hepatopancreas, hindgut, gills, heart, foregut, and nerve tissues. FITC staining was observed around the inner periphery of the nuclear membrane, with lighter staining in a more dispersed pattern within the nucleus. The probe did not hybridize with host tissues of uninfected spiny lobsters, nor did it cross-react with 4 other virus samples tested. This assay will facilitate our understanding of the pathogenesis of the viral disease and help in monitoring efforts directed at determining the prevalence of PaV1 in juvenile nurseries for this lobster

Simple Behavior of Primary Cross Sections for Low Mass Particles in p-pbar Collisions at y=0 and sqrt(s)=1.8 TeV

A set of inclusive cross sections at zero rapidity is presented for p-pbar interactions at center of mass energy sqrt(s)=1.8 TeV. Six particle cross sections are corrected for secondary contributions from decays of higher mass resonances in order to produce a set of primary cross sections. The primary cross sections per spin state are well described by d(sigma^p)/dy|_(y=0)= 0.721*(pi*lambdabar_(pi)^2)*exp(-m/T), where m is the particle rest mass, T=hbar*c/r_h, and r_h=0.97 fm. The deuterium production cross section is also described if r_h is replaced by r_A=r_h*A^(1/3). The same exponential in m and T describes primary charm fractions in e+e- collisions at least up to the J/Psi mass. There is no significant evidence for strangeness or charm suppression if only primary production of light hadrons is considered. There is evidence that the primary cross section for each particle may have the same value for pp and pbar-p collisions and that it may have nearly constant values between sqrt(s)=63 GeV and sqrt(s)=1800 GeV. Fits to the final state transverse momenta of the particles using a gas model favor a temperature T=132 MeV, a chemical potential mu=129 MeV, and a transverse flow of the gas with beta_f=0.27.Comment: 20 pages, 18 figure

Antineutrino interactions in the deuterium filled 15 foot bubble chamber

It is proposed to study the interactions of antineutrinos in deuterium. The initial request is for 300,000 pictures out of a total of one million pictures using the two-horn broad band system with 10{sup 13} protons (300 GeV) per pulse. We plan to use the external muon identifier. The physics motivation includes the following: (1) Search for charmed mesons. (2) Study of elastic and inelastic hyperon production. Only in anti-neutrino interactions can we study the {Delta}S = 1 charged current in quasi two-body interactions at energies and momentum transfers higher than that available in decays. (3) Study of the quasi elastic and pion production reactions. These data will be combined with the {nu}p data of our experiment (E-31). (4) Inclusive study of charged current interactions off neutrons and protons. (5) We will study the structure of the neutral current interaction. We will check the absence of {Delta}S = 1 neutral currents. (6) We will also look for di-muon events using the EMI. (7) We are interested in the possibility of inserting a three-radiation length metal plate in the bubble chamber to serve as a gamma converter and an electron detector

Neutrino interactions in the deuterium-neon 14 foot double bubble chamber

We propose to study the interactions of high energy neutrinos in the 14 foot bubble chamber. The target chamber to be filled with Deuterium and the surrounding region filled with nearly pure Neon. An exposure of one million pictures is requested, in order to map out the s and t dependences of the basic interaction in which neutrinos participate