Tamanho de explante na eficiência de indução de autotetraploides em bananeira.

A indução de duplicação de cromossomos tem sido uma ferramenta utilizada no melhoramento de bananeira para a produção de autotetraploides que serão utilizados como doadores de gametas 2n e que quando cruzados com diploides melhorados geram triploides secundários

Effects of leaf removal and shoot trimming on physiological and agronomic characteristics of Syrah grapevine in Northeast Brazil: preliminary results.

The São Francisco Valley, Northeast Brazil, is one of the most important wine regions with tropical conditions in the world. lt is located between parallels 8 and 1OoS, with an average annual temperature of 26.5 OC and 3,000 hours of sunshine per year. This work shows preliminary results on physiological and agronomic characteristics of Syrah vines.subjected to the practices of leaf removal and shoot trimming. The physiological and agronomical characteristics evaluated were the following: mass of cluster, mass of canes after pruning, mass of fresh and dry leaves, net photosynthesis (An), stomatal conductance (gs), intrinsic water use eficience (WUEi), leaf area (LA) and specific leaf area (SLA). The treatments did not show difJerences related to net photosynthesis (An), stomatal conductance (gs) and intrinsic water use ejJiciency (WUEi). The initial measurements of leaf area showed no difJerence between treatments. The individual leaf area was initially a.ffected by treatments, but during ripening these e.ffects were not revealed in the experimento Reverse e.ffects were observed for specific leaf area, which proved not to be a.ffected by treatments during the immature cluster phenological phase. The studies should be continued for three more seasons .in order to understand how the canopy management of Syrah, growing in a tropical environment, can injluence the agronomical and physiological plant behavior, and the implications for the quality of grapes and wines

Identificação de duplicatas de acessos de Mandioca (Manihot esculenta Crantz) com uso de dados morfológicos

Recursos Genético

Geração de mutantes de mandioca com uso de agentes químicos

Recursos Genético

Multiplicação de genótipos de bananeira diploides visando à duplicação de cromossomos

A bananicultura se destaca como uma atividade de grande importância econômica e social. A busca por novas cultivares por meio da seleção dentro dos recursos genéticos existentes, ou pela geração de novas cultivares através de hibridações constituem métodos tradicionais e eficientes, no entanto, as cultivares triploides de bananeira apresentam diferentes níveis de esterilidade, o que limita o sucesso na realização dos cruzamentos

Seleção de genótipos de mandioca com o gene waxy.

Alterações no gene GBSSI (Granule-bound starch synthase I) da mandioca (Manihot esculenta Crantz) originam o amido ceroso (tipo waxy - wx), que é caracterizado por apresentar até 5% de amilose. Este tipo de amido possui diversas aplicações na indústria de alimentos, e atualmente somente é obtido por meio de modificações químicas e físicas de alto custo. Portanto, o desenvolvimento de variedades com o amido ceroso é uma estratégia viável, embora seja necessário conhecer o germoplasma que possui alelos para o gene waxy, pois caso contrário as ações de pesquisa podem resultar em insucesso

Direct Preparation and Characterization of Copper Pentacyanonitrosylferrate Nanoparticles

The present work describes the preparation of nanoparticles of copper pentacyanonitrosyl complexes starting from the compound sodium nitroprusside. Copper pentacyanonitrosylferrate (NCuNP) nanoparticles were successfully synthesized by using deionized water and formamide as solvent. The material was characterized by Fourier-transforming infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV). The results revealed that the electronic spectra of NCuNP exhibited a broad intervalence charge transfer band at 685 nm. An XRD peak broadening pattern of the NCuNP was verified, indicating a particle decrease when formamide is used. The particle size of NCuNP is estimated to be 80 nm. The cyclic voltammogram of the modified graphite paste electrode with NCuNP showed two redox pairs with formal potentials Eθ′=0.36 V and Eθ′=0.78 V (ν=20 mV s−1; KCl 1.0 M), attributed to the redox process CuI/ CuII and [FeII(CN)5NO]/[FeIII(CN)5NO], respectively. The graphite paste electrode with NCuNP presents electrocatalytic response for Sulfite determination

Specialized active leprosy search strategies in an endemic area of the Brazilian Amazon identifies a hypermutated Mycobacterium leprae strain causing primary drug resistance

INTRODUCTION: Leprosy, an infectious disease caused by Mycobacterium leprae, remains a public health concern in endemic countries, particularly in Brazil. In this study, we conducted an active surveillance campaign in the hyperendemic city of Castanhal in the northeastern part of the state of Pará using clinical signs and symptoms combined with serological and molecular tools to diagnose new cases and to identify drug resistance of circulating M. leprae strains and their distribution in the community. METHODS: During an active surveillance of one week, we enrolled 318 individuals using three different strategies to enroll subjects for this study: (i) an active survey of previously treated cases from 2006 to 2016 found in the Brazil National Notifiable Disease Information System database (n = 23) and their healthy household contacts (HHC) (n = 57); (ii) an active survey of school children (SC) from two primary public schools in low-income neighborhoods (n = 178), followed by visits to the houses of these newly diagnosed SC (n = 7) to examine their HHC (n = 34) where we diagnosed additional new cases (n = 6); (iii) and those people who spontaneously presented themselves to our team or the local health center with clinical signs and/or symptoms of leprosy (n = 6) with subsequent follow-up of their HHC when the case was confirmed (n = 20) where we diagnosed two additional cases (n = 2). Individuals received a dermato-neurological examination, 5 ml of peripheral blood was collected to assess the anti-PGL-I titer by ELISA and intradermal earlobe skin scrapings were taken from HHC and cases for amplification of the M. leprae RLEP region by qPCR. RESULTS: Anti-PGL-I positivity was highest in the new leprosy case group (52%) followed by the treated group (40.9%), HHC (40%) and lowest in SC (24.6%). RLEP qPCR from SSS was performed on 124 individuals, 22 in treated cases, 24 in newly diagnosed leprosy cases, and 78 in HHC. We detected 29.0% (36/124) positivity overall in this sample set. The positivity in treated cases was 31.8% (7/22), while in newly diagnosed leprosy cases the number of positives were higher, 45.8% (11/23) and lower in HHC at 23.7% (18/76). Whole genome sequencing of M. leprae from biopsies of three infected individuals from one extended family revealed a hypermutated M. leprae strain in an unusual case of primary drug resistance while the other two strains were drug sensitive. DISCUSSION: This study represents the extent of leprosy in an active surveillance campaign during a single week in the city of Castanhal, a city that we have previously surveyed several times during the past ten years. Our results indicate the continuing high transmission of leprosy that includes fairly high rates of new cases detected in children indicating recent spread by multiple foci of infection in the community. An unusual case of a hypermutated M. leprae strain in a case of primary drug resistance was discovered. It also revealed a high hidden prevalence of overt disease and subclinical infection that remains a challenge for correct clinical diagnosis by signs and symptoms that may be aided using adjunct laboratory tests, such as RLEP qPCR and anti-PGL-I serology