The Likelihood Ratio Test and Full Bayesian Significance Test under small sample sizes for contingency tables

Hypothesis testing in contingency tables is usually based on asymptotic results, thereby restricting its proper use to large samples. To study these tests in small samples, we consider the likelihood ratio test and define an accurate index, the P-value, for the celebrated hypotheses of homogeneity, independence, and Hardy-Weinberg equilibrium. The aim is to understand the use of the asymptotic results of the frequentist Likelihood Ratio Test and the Bayesian FBST -- Full Bayesian Significance Test -- under small-sample scenarios. The proposed exact P-value is used as a benchmark to understand the other indices. We perform analysis in different scenarios, considering different sample sizes and different table dimensions. The exact Fisher test for $2 \times 2$ tables that drastically reduces the sample space is also discussed. The main message of this paper is that all indices have very similar behavior, so the tests based on asymptotic results are very good to be used in any circumstance, even with small sample sizes

Silk bilayer scaffolds can induce fast integration with subchondral bone and support cartilage repair

Publicado em : J Tissue Eng Regen Med 2014; 8 (Suppl. 1)Introduction: Osteochondral defect (OCD) regeneration presents major challenges in orthopedics. Since healing of cartilage and bone should be simultaneously considered, ideal scaffolds should be those that can mimic both tissues properties. In this study, bilayered silk and silk-nano calcium phosphate (Silk/Silk-NanoCaP) scaffolds with tailored mechanical properties were developed for OCD tissue engineering application. Materials and methods: Aqueous silk solution (16%) was prepared.1 Nano calcium phosphate particles (16%) were synthesized in the silk solution (Silk-NanoCaP).2 The bony layer was prepared by addition of NaCl particles (500–1000 lm) into the Silk-NanoCaP suspension. After drying for 2 days and salt-leaching overnight, silk solution was added on top of the bony layer using the same procedure to produce the chondral layer. The !nal scaffolds were evaluated through in vitro culture of rabbit bone marrow stromal cells (RBMSCs) for 2 weeks, and in vivo implantation in a rabbit knee OCD for 4 weeks. Results: The RBMSCs cultured in the scaffolds presented increasing viability from day 1 to day 7 by MTS assay. Good adhesion and migration of the RBMSCs in the scaffolds were achieved, as observed under the scanning electron microscope. Cell proliferation was observed from day 7 to day 14 as determined by DNA quanti!cation. The bony layer induced higher alkaline phosphatase level as compared to the chondral layer, in osteogenic condition. Histological analysis (H&E) showed that the bilayered scaffolds integrated well with the host tissue, after 4 weeks of implantation in a critical size OC defect (Fig. 1). Abundant new bone formation was detected in the Silk-NanoCaP layer. Cartilage regeneration occurred in the silk layer. Discussion and conclusions: The bilayered scaffolds favored the attachment, proliferation, and differentiation of RBMSCs. The bony layer of the bilayered scaffolds possessed osteoconductive properties. The bilayered scaffolds were biocompatible in vitro and in vivo. These scaffolds also induced both subchondral bone regeneration and supported cartilage regeneration, thus showing great promise in OCD regeneration. Acknowledgments: The authors thank FCT projects Tissue2Tissue and OsteoCart, and the FP7 Programme POLARIS. Yan LP was awarded a FCT PhD scholarship. Investigador FCT program (IF/00423/2012) and (IF/00411/2013) are also greatly acknowledged. Disclosure: The authors declare that there is no con"ict of interest

New Criteria of Indication and Selection of Patients to Cochlear Implant

Numerous changes continue to occur in cochlear implant candidacy. In general, these have been accompanied by concomitant and satisfactory changes in surgical techniques. Together, this has advanced the utility and safety of cochlear implantation. Most devices are now approved for use in patients with severe to profound unilateral hearing loss rather then the prior requirement of a bilateral profound loss. Furthermore, studies have begun utilizing short electrode arrays for shallow insertion in patients with considerable low-frequency residual hearing. This technique will allow the recipient to continue to use acoustically amplified hearing for the low frequencies simultaneously with a cochlear implant for the high frequencies. The advances in design of, and indications for, cochlear implants have been matched by improvements in surgical techniques and decrease in complications. The resulting improvements in safety and efficacy have further encouraged the use of these devices. This paper will review the new concepts in the candidacy of cochlear implant. Medline data base was used to search articles dealing with the following topics: cochlear implant in younger children, cochlear implant and hearing preservation, cochlear implant for unilateral deafness and tinnitus, genetic hearing loss and cochlear implant, bilateral cochlear implant, neuropathy and cochlear implant and neural plasticity, and the selection of patients for cochlear implant

Quantitative structure-activity relationships (QSAR) studies of bisbenzamidines with antifungal activity

This paper describes 2D-QSAR and 3D-QSAR studies against Candida albicans and Cryptococcus neofarmans for a set of 20 bisbenzamidines. In the studies of 2D-QSAR with C. albicans it was obtained a correlation between log MIC-1 and lipolo component-Z (r² = 0.68; Q² = 0.51). In the case of C. neofarmans a correlation between log MIC-1 and lipolo component-Z and of Balaban index (r² = 0.85; Q² = 0.6) was obtained. 3D-QSAR studies using CoMFA showed that the steric fields contributed more to the predicted activities for Candida albicans (94.9%) and Cryptococcus neofarmans (97.9%).FAPEMIGCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)CNP

Prior Exercise Reduces Fast-Start Duration and End-Spurt Magnitude during Cycling Time-Trial

We examined the pacing strategy and the magnitude of the end spurt during a 200-kJ cycling time trial performed 12–14 h after an exercise protocol designed to reduce muscle glycogen content. 9 physically-active men performed 5 familiarization sessions and 2 experimental 200-kJ time trials in either a control condition (CON) or after an exercise protocol performed the previous evening that was designed to induce muscle glycogen depletion (EP). Mean total time was faster and power output was higher in the CON than in the EP (P<0.01). A fast-start was maintained until the 50-kJ section in CON, but only the 25-kJ section for EP (P<0.05). The power outputs during the 50-, 150- and 200-kJ sections, and the magnitude of the end-spurt, were significantly higher for the CON than for the EP condition (P<0.05). There was no significant difference in the rating of perceived exertion (overall feeling and feeling in legs) between conditions. In conclusion, a protocol designed to decrease muscle glycogen stores reduced the duration of the fast-start and the magnitude of the end spurt during a 200-kJ cycling time trial, impairing the overall performance

New endoscopic procedure for bladder wall closure: results from the porcine model

Upper urinary tract urothelial carcinomas are usually managed by radical nephroureterectomy (RNU), often followed by intravesical chemotherapy to minimize recurrence. Open surgery is the gold standard procedure for RNU, but it associates with high morbidity, and it has been increasingly replaced by minimally invasive strategies, such as laparoscopy and endoscopy. Although effective, endoscopic ureteral excision leaves the bladder unsutured, increasing the risk of tumor spillage, and precluding the immediate administration of intravesical chemotherapy. Here we describe a new method to close the bladder wall after ureteral excision, using barbed sutures via the endoscopic access. Our results in 8 female pigs demonstrate that this method is effective to close the bladder wall. The procedure was completed in a median time of 24 min, and no adverse events were registered in the follow-up or at the three-week necropsy. This technique improves a previous approach described by our group because the device is more flexible and allows to tie the knots inside the bladder. Barbed sutures have been used in the clinical practice for other types of surgeries, and therefore this method can further be adapted to human patients with no safety concerns. Its use may allow to administer intravesical chemotherapy, which reduces tumor recurrence and improves patient outcomes.The authors acknowledge Ana Goios for support in manuscript writing and technical editing, and for producing original illustrations for Figure 3. Doctoral grant of CUF Jose de Mello Saude

A novel modular voltage balancing topology for active battery management system

This paper proposes a novel modular voltage balancing topology for an active battery management system (BMS). The proposed topology consists of two power stages. The first stage is composed by a switch network, which is responsible for selecting the battery that will provide energy and the battery that will receive energy. In the switch network, for each battery, are used two cells, which allow the operation with bidirectional current flow and bipolar voltage. The second stage is composed by a capacitor used as energy storage element, which consists in the component used to link both batteries. The switch network control and its interaction with the energy storage element is performed by a digital controller. The paper presents the main computer simulations, as well as the main experimental results obtained to validate the proposed voltage balancing topology for an active BMS.This work has been supported by FCT - Fundação para a Ciência e Tecnologia in the scope of the project: PEst-UID/CEC/00319/2013. Mr. Vítor Monteiro was supported by the doctoral scholarship SFRH/BD/80155/2011 granted by the FCT agency

Células de linhagem McCoy como um possível modelo contendo receptores CD4+ para estudos da replicação do HIV

Several studies have recently shown the use of recombinant rabies virus as potential vector-viral vaccine for HIV-1. The sequence homology between gp 120 and rabies virus glycoprotein has been reported. The McCoy cell line has therefore been used to show CD4+ or CD4+ like receptors. Samples of HIV-1 were isolated, when plasma of HIV-1 positive patients was inoculated in the McCoy cell line. The virus infection was then studied during successive virus passages. The proteins released in the extra cellular medium were checked for protein activity, by exposure to SDS Electrophoresis and blotting to nitro-cellulose filter, then reacting with sera of HIV positive and negative patients. Successive passages were performed, and showed viral replication, membrane permeabilization, the syncytium formation, and the cellular lysis (cytopathic effect). Flow cytometry analysis shows clear evidence that CD4+ receptors are present in this cell line, which enhances the likelihood of easy isolation and replication of HIV. The results observed allow the use of this cell line as a possible model for isolating HIV, as well as for carrying out studies of the dynamics of viral infection in several situations, including exposure to drugs in pharmacological studies, and possibly studies and analyses of the immune response in vaccine therapies.Recentes estudos demonstraram o uso do vírus raiva como modelo vetor para produzir vacinas expressando as glicoproteínas do vírus HIV-1. A homologia na seqüência entre gp120 do vírus HIV-1 e a glicoproteína G do vírus rábico já foi previamente relatada. Devido a estes fatos a linhagem de célula McCoy utilizada com sucesso para a replicação do vírus rábico foi utilizada para demonstrar a replicação do HIV-1. Amostra de HIV-1 foi isolada de plasma de um paciente soro positivo e inoculada em células de linhagem McCoy e então a infecção viral foi estudada em passagens sucessivas do vírus nesta célula. As proteínas liberadas no meio extra celular foram analisadas quanto a atividade biológica pela técnica de eletroforese em gel de poliacrilamida e imunotransferência em membrana de nitro-celulose reagindo com soros positivos para HIV-1 e soros de pacientes negativos. As passagens sucessivas do HIV-1 em células demonstraram a replicação viral, o aumento da permeabilidade da membrana citoplasmática, a formação de sinsício e lise celular. Análises com citometria de fluxo mostraram com clara evidência a presença de receptores CD4+ o que possivelmente deve ser a causa que possibilita a facilidade do isolamento e replicação do vírus HIV-1 nesta célula. Concluindo os resultados observados permitem utilizar esta linhagem celular como um possível modelo para isolamentos de HIV, bem como realizar estudos da dinâmica de infecção viral em diversas situações inclusive de exposição a drogas em estudos farmacológicos, e talvez estudos e análises da resposta imune em terapias vacinais

A New Spin on Galactic Dust

We present a new puzzle involving Galactic microwave emission and attempt to resolve it. On one hand, a cross-correlation analysis of the WHAM H-alpha map with the Tenerife 10 and 15 GHz maps shows that the well-known DIRBE correlated microwave emission cannot be dominated by free-free emission. On the other hand, recent high resolution observations in the 8-10 GHz range with the Green Bank 140 ft telescope by Finkbeiner et al. failed to find the corresponding 8 sigma signal that would be expected in the simplest spinning dust models. So what physical mechanism is causing this ubiquitous dust-correlated emission? We argue for a model predicting that spinning dust is the culprit after all, but that the corresponding small grains are well correlated with the larger grains seen at 100 micron only on large angular scales. In support of this grain segregation model, we find the best spinning dust template to involve higher frequency maps in the range 12-60 micron, where emission from transiently heated small grains is important. Upcoming CMB experiments such as ground-based interferometers, MAP and Planck LFI with high resolution at low frequencies should allow a definitive test of this model.Comment: Minor revisions to match accepted ApJ version. 6 pages, 4 figs. Color figures and more foreground information at http://www.hep.upenn.edu/~angelica/foreground.html#spin or from [email protected]