7 research outputs found
Increased basal K<sub>ATP</sub> activity in both hetDel and hetT, del channels.
<p><sup>86</sup>Rb<sup>+</sup> efflux of mutant Kir6.2 subunits coexpressed with WT subunits in 1∶1 DNA ratio, under metabolic inhibition (A) and in basal states (B). Data points indicate means ± SEM of n = 5. * indicates P<0.05 compared with WT by One-Way ANOVA analysis. NT  =  not transfected (no statistic given).</p
An Electrically-Driven GaAs Nanowire Surface Plasmon Source
Over the past decade, the properties of plasmonic waveguides
have
extensively been studied as key elements in important applications
that include biosensors, optical communication systems, quantum plasmonics,
plasmonic logic, and quantum-cascade lasers. Whereas their guiding
properties are by now fairly well-understood, practical implementation
in chipscale systems is hampered by the lack of convenient electrical
excitation schemes. Recently, a variety of surface plasmon lasers
have been realized, but they have not yet been waveguide-coupled.
Planar incoherent plasmonic sources have recently been coupled to
plasmonic guides but routing of plasmonic signals requires coupling
to linear waveguides. Here, we present an experimental demonstration
of electrically driven GaAs nanowire light sources integrated with
plasmonic nanostrip waveguides with a physical cross-section of 0.08λ<sup>2</sup>. The excitation and waveguiding of surface plasmon-polaritons
(SPPs) is experimentally demonstrated and analyzed with the help of
full-field electromagnetic simulations. Splitting and routing of the
electrically generated SPP signals around 90° bends are also
shown. The realization of integrated plasmon sources greatly increases
the applicability range of plasmonic waveguides and routing elements
Decreased K<sub>ATP</sub> activity in both homDel and homT, del channels.
<p>Representative <sup>86</sup>Rb<sup>+</sup> efflux profile comparing untransfected COSm6 cells (Un) and cells transfected with WT, homS225T, homDel and homT, del channels in metabolic inhibition (A) and in basal conditions (B). Data points indicate means ± SEM of n = 4. NT  =  not transfected.</p
Expression of WT and homomeric mutant channels in patch clamp recordings.
<p>(A) representative currents recorded by inside-out excised patch-clamp technique from COSm6 cells expressing WT channels and various mutant channels, at +50 mV pipette potential. Patches were exposed to different concentrations of Mg-free ATP as indicated. Dashed line indicates zero current (WT and homS225T channels) or zero channel level (homDel and homT, del channels). (B) Western Blot of Flag-tagged SUR1 (fSUR1) from various constructs are indicated. The mature (cell surface) complex-glycosylated bands and immature core-glycosylated bands are indicated as upper bands (white arrow) and lower bands (black arrow) respectively. NT = not transfected.</p
Homology modeling of Kir6.2 from Kir2.2 structure with PyMOL software.
<p>(A) S225T is colored in yellow and deleted amino acids 226–232 (-PEGEVVP-) are colored in orange. (B) E227 and E229 are colored and labeled in red while R192 and R314 are colored and labeled in blue. The structure model reveals the possible interaction between the deleted amino acid P232 (orange spheres) and V319 in the proposed Kir6.2 Ankyrin-B binding site (a.a. 316 to a.a. 323 –VPIVAEED- colored in magenta).</p
Heterozygous S225T, deletion channels display higher channel open probability, assessed by the ‘PIP<sub>2</sub>’ method.
<p>(A) Representative currents recorded by inside-out excised patch-clamp technique from COSm6 cells expressing WT channels and hetT, del mutants. Patches were exposed to different concentrations of Mg-free ATP and PIP<sub>2</sub> as indicated. (B) Mean estimated Po for various channels: WT (0.53±0.04); homS225T (0.62±0.04); hetS225T (0.59±0.03); hetDel (0.64±0.04); hetT, del (0.66+0.02). * indicates statistically significant difference compared with WT (Student's t-test, p-value <0.05). n = 6–12.</p
Decreased ATP sensitivity in both hetDel and hetT, del channels.
<p>(A) Representative currents recorded by inside-out excised patch-clamp technique from COSm6 cells expressing WT channels and hetT, del mutants. Patches were exposed to different concentrations of Mg-free ATP as indicated. (B) ATP dose-response relationships, fit by Hill equation as described in methods. Data points indicate means ± SEM of n = 5–7 patches. * indicates P<0.05 compared with WT by One-Way ANOVA analysis. The fitted K<sub>1/2</sub> for WT, homS225T, hetS225T, hetDel, and hetT, del channels are 13.75, 25.06, 14.8, 22.87 and 43.94 (in μM) and the Hill coefficients are 1.6, 1.3, 1.1, 1.28 and 1.2, respectively.</p