Evaluation of cytotoxic and genotoxic activities in the bone marrow of mice treated with sulfonamide chalcone N-{4-[3-(4-nitrophenyl)prop-2-enoyl]phenyl}-benzenesulfonamide (CPN) based on MNPCE and PCE/NCE frequency.

<p>¹ Negative control: dimethylsulfoxide (DMSO) 0.1 mL/10 g body weight (b.w.).</p><p>² Positive control: mitomycin C (MMC) 4 mg/kg (80% LD₅₀).</p><p>All values are mean ± SD of five mice.</p><p>³ Statistical analysis: one-way ANOVA and the Tukey’s test.</p><p>⁴ Statistical analysis: chi-square (χ^<b>2</b>) test.</p><p>Mean values followed by the same letter in the column do not present significant difference at 5% probability.</p><p>^{a, b, c, d} Letter in common in the same column do not present significant difference (<i>p</i> > 0.05). Different letters in the same column present significant difference (<i>p</i> < 0.05).</p><p>Evaluation of cytotoxic and genotoxic activities in the bone marrow of mice treated with sulfonamide chalcone N-{4-[3-(4-nitrophenyl)prop-2-enoyl]phenyl}-benzenesulfonamide (CPN) based on MNPCE and PCE/NCE frequency.</p

Means ± standard deviation (SD) of histidine revertant colonies, mutagenic index (MI), and inhibition percentage (IP) of mutagenicity for two tester strains of <i>Salmonella typhimurium</i>, TA98 and TA100, after treatment with different doses of sulfonamide chalcone N-{4-[3-(4-nitrophenyl)prop-2-enoyl]phenyl}-benzenesulfonamide (CPN).

<p>¹ Negative control: 20 μL dimethylsulfoxide (DMSO).</p><p>² Positive control: 0.5 μg 4-nitroquinoline 1-oxide (4-NQO)/plate for TA98 and 1.5 μg sodium azide/plate for TA100.</p><p>All values are means ± SD of three independent experiments.</p><p>³ Statistical analysis: one-way ANOVA and the Tukey’s test.</p><p>^{a, b, c, d} Letter in common in the same column do not present significant difference (<i>p</i> > 0.05). Different letters in the same column present significant difference (<i>p</i> < 0.05).</p><p>Means ± standard deviation (SD) of histidine revertant colonies, mutagenic index (MI), and inhibition percentage (IP) of mutagenicity for two tester strains of <i>Salmonella typhimurium</i>, TA98 and TA100, after treatment with different doses of sulfonamide chalcone N-{4-[3-(4-nitrophenyl)prop-2-enoyl]phenyl}-benzenesulfonamide (CPN).</p

Yield, physical state, melting point, purity, and spectroscopic data (GS-MS, IR, ¹H-NMR) of N-(4-acetylphenyl)benzenesulfonamide (1) and N-{4-[(<i>E</i>)-3-(4-nitrophenyl)prop-2-enoyl]phenyl}-benzenesulfonamide (2).

<p>¹ Retention time.</p><p>² Mobile phase used: CH₃CN:aqueous buffer containing chloroacetic acid 0.1% (55:45).</p><p>Yield, physical state, melting point, purity, and spectroscopic data (GS-MS, IR, ¹H-NMR) of N-(4-acetylphenyl)benzenesulfonamide (1) and N-{4-[(<i>E</i>)-3-(4-nitrophenyl)prop-2-enoyl]phenyl}-benzenesulfonamide (2).</p